Blimp1

Manufactured by BD

Sourced in United States

Blimp1 is a laboratory equipment used for the detection and measurement of various biological and chemical compounds. It utilizes a specialized detection mechanism to provide accurate and reliable results. The core function of Blimp1 is to assist researchers and scientists in their analytical processes, though its specific applications may vary depending on the user's needs.

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Lab products found in correlation

Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions) Bodipy 493 503, by Thermo Fisher Scientific (1 mentions) Bodipy fl c16, by Thermo Fisher Scientific (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by BD (1 mentions) Annexin 5, by BD (1 mentions) Ctla 4, by BioLegend (1 mentions) Fabp5, by R&D Systems (1 mentions) Pparγ, by Abcam (1 mentions) Bcl xl, by Abcam (1 mentions) Bcl 2, by Cell Signaling Technology (1 mentions) Ifn γ, by BD (1 mentions) Live dead viability cytotoxicity kit, by Thermo Fisher Scientific (1 mentions) Klrg1, by BioLegend (1 mentions) Pma ionomycin, by BioLegend (1 mentions) Eomes, by R&D Systems (1 mentions) T bet, by BioLegend (1 mentions) Nfatc1, by BioLegend (1 mentions) Tim 3, by BioLegend (1 mentions) Facscanto 2, by BD (1 mentions) Anti cd3 mab, by BD (1 mentions)

Spelling variants of this product

Blimp1 (sc-47732, (3 citations) Blimp1-PE (2 citations)

3 protocols using blimp1

Multiparametric Immune Profiling of T Cells

Cited 1 time

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Cells were stained with CD3 (BioLegend, clone: HIT3a), CD8 (BioLegend, clone: SK1), CD45RO (BioLegend, clone: UCHL1), T-bet (BioLegend, clone: 4B10), Eomes (R&D Systems, clone: 644730), NFATC1 (BioLegend, clone: 7A6), PD-1 (BioLegend, clone: EH12.2H7), TIM-3 (BioLegend, clone: F38-2E2), CTLA4 (BioLegend, clone: L3D10), LAG3 (BioLegend, clone: 11C3C65), Bcl-XL (Abcam, clone:7B2.5), FABP5 (R&D Systems, clone: 311215)，CPT1α (Proteintech, 15184-1-AP), CD137 (BioLegend, clone: 4B4-1), mito Tracker Red CMXROS (Invitrogen, M7512), TMRM (Invitrogen, M20036), LIVE/DEAD Viability/Cytotoxicity Kit (Invitrogen, L3224), CD27 (BioLegend, clone: M-T271), CD127 (eBioscience, clone: eBioRDR5), IFNγ (BD Pharmingen, clone: B27), TCF7 (BioLegend, clone: 7F11A10), IRF4 (BioLegend, clone: IRF4.3E4), Blimp1 (BD Pharmingen, clone: 6D3), BCL2 (Cell Signaling Technology, clone: 124), Ki67 (Abcam, clone: B126.1), PPARγ (Abcam, clone: EPR18516), Annexin V (BD Pharmingen, 556547), CFSE (BD Pharmingen, 565082), KLRG1 (BioLegend, clone: 2F1/KLRG1), as well as BODIPY 493/503 (Invitrogen, D3922) and BODIPY FL C16 (Invitrogen, D3821). For intracellular staining, cells were given PMA/ionomycin (BioLegend, 423303) re-stimulation and then intracellular staining was performed as previously described.6 (link) FACS analysis was performed on a BD FACS Aria II flow cytometer and analyzed by FlowJo V.6 software.

Liu F., Liu W., Zhou S., Yang C., Tian M., Jia G., Wang H., Zhu B., Feng M., Lu Y., Qiao T., Wang X., Cao W., Wang X., Shi Y, & Wu D. (2020). Identification of FABP5 as an immunometabolic marker in human hepatocellular carcinoma. Journal for Immunotherapy of Cancer, 8(2), e000501.

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Intracellular Cytokine Staining Protocol

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The cell staining procedure used in this study was described previously (Wu et al., 2018a (link)). For intracellular cytokine staining, the cells were restimulated for 12 h with 10 g/ml CagA (Linc-Bio Science, Shanghai, China), 10 μg/ml VacA (Linc-Bio Science, Shanghai, China), anti-CD3 mAb (Clone UCHT1, BD), anti-CD28 mAb (Clone CD28.2, BD) and 3 μg/ml brefeldin A (eBioscience, CA, USA). Intracellular cytokines were stained using the intracellular fixation/permeabilization buffer set (eBioscience, CA, USA). Flow cytometric analysis was performed on FACS Canto II (BD, NJ, USA), and the data were analyzed using FlowJo software (Tree Star). The following anti-human antibodies were purchased from eBioscience, BD Biosciences or Biolegend (CA, USA): CD3 (Clone UCHT1, BD), CD4 (Clone L200, BD), CD8 (Clone RPA-T8, BD), CD45RO (Clone UCHL1, BD), CD69 (Clone FN50, Biolegend), CD103 (Clone Ber-ACT8, Biolegend), CCR7 (Clone 150503, BD), CCR9 (Clone L053E8, Biolegend), CXCR3 (Clone 1C6/CXCR, BD), E-Cadherin (Clone DECMA-1, Biolegend), TNF (Clone MAb11, eBioscience), IFN-γ (Clone 4S.B3, eBioscience), IL-17a (Clone BL168, Biolegend), T-bet (Clone O4-46, BD), and Blimp1 (Clone 6D3, BD), ZAP70 Phospho (Tyr319) (Clone 1503310, Biolegend).

Chen P., Ming S., Lao J., Li C., Wang H., Xiong L., Zhang S., Liang Z., Niu X., Deng S., Geng L., Wu M., Wu Y, & Gong S. (2020). CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4+ T Cell in Helicobacter pylori-Positive Gastritis. Frontiers in Cellular and Infection Microbiology, 10, 436.

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Single Cell Isolation and Intracellular Staining

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Single cell suspensions of spleen, lymph node, or femur bone marrow were prepared and processed as previously described (44) (link). For intracellular staining, cells were additionally incubated with eFluor780 viability dye (ThermoFisher) for 15 minutes and then processed using the Foxp3 staining kit (ThermoFisher) per manufacturer's protocol. Cells were then stained for transcription factors Blimp1 (BD Biosciences) or Foxp3 (eBioscience) for 30 minutes. All flow cytometry was performed on a four-laser FACS Aria III using Diva 8.0 software (BD). Downstream analyses, including index sort plate-mapping and tSNE visualization, were performed using FlowJo software (Version 10, TreeStar).

Miller K.J., Shuparski A.G., Higgins B.W., Sharma S., Milpied P., McHeyzer‐Williams L.J., & McHeyzer‐Williams M.G. (2022). Follicular helper T cells expressing Blimp1 are specialized for plasma cell differentiation.

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