The DPSCs were collected at the Intellectual Biointerface Engineering Center, Dental Research Institute, College of Dentistry, Seoul National University. All experiments related to the DPSCs were approved by the Seoul National University Institutional Animal Care and Use Committee (SNU-120427-2-2). The cells were cultured in α-minimum essential medium (MEM) containing 10% fetal bovine serum (FBS, Welgene Inc., Gyeongsan, Korea), 10 mM ascorbic acid (l -ascorbic acid, Simga-Aldrich, St. Louis, MO, USA), antibiotics (Welgene Inc., Gyeongsan, Korea), and sodium bicarbonate (DUKSAN, Seoul, Korea) at 37 °C in a humidified atmosphere of 5% CO2 (Steri-Cycle 370 Incubator, Thermo Fisher Scientific, Waltham, MA, USA). The medium was changed every other day. When the cells became confluent, they were detached with 1 mL trypsin-EDTA solution (LS 015-10, Welgene Inc., Gyeongsan, Korea), counted, and passaged.
Ls 015 10
Manufactured by Welgene
The LS 015-10 is a laboratory centrifuge designed for general-purpose sample separation. It has a maximum speed of 10,000 rpm and a maximum relative centrifugal force (RCF) of 15,000 x g. The centrifuge can accommodate a variety of sample tube sizes and types, making it suitable for a wide range of applications in research and clinical laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Steri cycle 370 incubator, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
Sodium bicarbonate, by Duksan Pure Chemicals (1 mentions)
Lm001 56, by Welgene (1 mentions)
Lm001 05, by Welgene (1 mentions)
Ldn 57444, by Selleck Chemicals (1 mentions)
Ls203 01, by Welgene (1 mentions)
Lm011 01, by Welgene (1 mentions)
2 protocols using ls 015 10
1
Dental Pulp Stem Cell Isolation and Culture
2
Cell Culture and Glucose Starvation Protocol
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SNU668, NCI-N87, MKN1, and SNU601 were maintained in Roswell Park Memorial Institute 1640 medium (RPMI1640, LM011-01; Welgene, Korea) containing 10% fetal bovine serum (FBS, 26140-079; Gibco, USA) and 1% antibiotics (LS203-01; Welgene) at 37°C in a humidified 5% CO2 incubator. HEK 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, LM001-05; Welgene) containing 10% FBS and 1% antibiotics at 37°C in a humidified 5% CO2 incubator. Trypsin-EDTA 0.25% (LS015-10; Welgene)was used for cell dissociation. The glucose-deprived medium was prepared by mixing glucose-free DMEM (LM001-56; Welgene) with high glucose DMEM (LM001-05; Welgene) in a 9:1 ratio to make 2.5 mM glucose concentration, followed by the addition of 10% FBS and 1% antibiotics. Complete high glucose DMEM with 25 mM glucose (LM001-05; Welgene) containing 10% FBS and 1% antibiotics was used as a control for the glucose starvation condition. TCID (B1467; APExBIO, USA) and LDN-57444 (S7135; Selleckchem, USA) were dissolved in DMSO and were treated for 24 h in the experiments.
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