The videos of the enzymatic micromotors motion were recorded using the camera (Hamamatsu Digital Camera C11440) of an inverted optical microscope (Leica DMi8). The 63× water immersion objective was used to record the micromotors placed on a glass slide, thoroughly mixed with the water solutions of substrate at a specific concentration selected to cover the range at which these enzymes were active and showed the Michaelis-Menten growth kinetics, as reported in BRENDA, the Comprehensive Enzyme Information System (https://www.brenda-enzymes.org/ ). The glass slide was covered with a coverslip and videos of 25 FPS and 30–35 s were recorded up to the first 3 min after mixing. For each enzymatic micromotor, 19–22 HSMM were recorded for each different concentration of substrate, inhibitor, and for no compound present.
Digital camera c11440
Manufactured by Leica
Sourced in Germany
The Digital Camera C11440 is a high-performance digital camera designed for laboratory and industrial applications. It features a large image sensor that captures detailed images with high resolution and sensitivity. The camera is capable of capturing images and video at various resolutions and frame rates, making it suitable for a wide range of imaging tasks.
Automatically generated - may contain errors
Lab products found in correlation
Nova nanosem 230, by Thermo Fisher Scientific (1 mentions)
Em 912, by Zeiss (1 mentions)
Varioskan lux fluorescence spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Zetasizer nano s, by Malvern Panalytical (1 mentions)
96 well imaging plate, by BD (1 mentions)
Fluo 4, by BD (1 mentions)
Dmi8 inverted microscope, by Leica (1 mentions)
5 protocols using digital camera c11440
1
Enzymatic Micromotors Motion Analysis
2
Estimating Ciliate Prey Density Using Video Analysis
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To estimate the prey density, we used video analysis (Pennekamp, Schtickzelle, & Petchey, 2015 ). In a custom counting chamber, we placed 700 µl of the 1 ml previously used to count the predators and made a 5‐s video at 25 frames per second of ~50 µl of the 700 µl using a camera (Hamamatsu Digital camera C11440) attached to a microscope (Leica M205C, 0.63X) and relevant software (HCImage Live version 4.0.6.3). The videos were analyzed using the BEMOVI package; this software isolates moving particles (here, ciliates), reconstructs their trajectories, and assigns trajectories to species based on morphological traits (Pennekamp et al., 2017 ). The customized counting chamber had a 0.6‐mm depth (compared to 1 mm for a Sedgewick rafter slide) to reduce the vertical movement of individuals during the video measurement and therefore increase the accuracy of measured morphological traits.
3
Characterization of Urease Micromotors
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SEM images were captured
by a FEI NOVA
NanoSEM 230. TEM images were captured by a Zeiss EM 912. The zeta
potential (ζ potential) measurements were performed with a Zetasizer
Nano S from Malvern Panalytical. The hydrodynamic radius and diffusion
coefficient measurements were performed using a Möbius from
Wyatt Technology. The optical videos of urease micromotors were recorded
using the camera (Hamamatsu digital camera C11440) of an inverted
optical microscope (Leica DMi8). The optical density (OD) of the antibacterial
assays was measured at 600 nm in a Thermo Scientific Varioskan LUX
fluorescence spectrophotometer.
by a FEI NOVA
NanoSEM 230. TEM images were captured by a Zeiss EM 912. The zeta
potential (ζ potential) measurements were performed with a Zetasizer
Nano S from Malvern Panalytical. The hydrodynamic radius and diffusion
coefficient measurements were performed using a Möbius from
Wyatt Technology. The optical videos of urease micromotors were recorded
using the camera (Hamamatsu digital camera C11440) of an inverted
optical microscope (Leica DMi8). The optical density (OD) of the antibacterial
assays was measured at 600 nm in a Thermo Scientific Varioskan LUX
fluorescence spectrophotometer.
4
Micromotors Capture Urease Kinetics
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The motion of the urease micromotors was recorded using the camera (Hamamatsu Digital Camera C11440) of an inverted optical microscope (Leica DMi8). The 63x water immersion objective was used to record the micromotors placed on a glass slide, thoroughly mixed with the specific solutions containing 200 mM urea, selected to cover the range at which these enzymes are active and show the Michaelis-Menten growth kinetics, as reported in BRENDA, the Comprehensive Enzyme Information System (https://www.brenda-enzymes.org/ ). The glass slide was covered with a coverslip, and videos of 25 FPS and 30-35 seconds were recorded up to the first 3 min after mixing. At least, 20 urease micromotors were recorded for each different condition.
5
Calcium Oscillations in KYSE-150 Cells
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KYSE-150 cells were seeded in black 96-well plate with clear bottom. After attachment, cells were treated with RP4010 and afatinib combined or separately at different concentrations (detail shows in Table 1) for 4 h. For RP4010, the concentration varied from 5 µM to 0.25 µM.
The concentration of afatinib varied from 5 µM to 0.25 µM. For the combination of RP4010 and afatinib, the ratio of RP4010 to afatinib was 2:1. KYSE-150 cells were loaded with 5 µM Fluo-4 in 96-well imaging plates (BD Falcon, NJ) at room temperature for 20 min. After washing, cells were kept in culture medium without phenol red. The intensity of fluorescent signals were recorded by Hamamatsu digital camera C11440 complemented with DMi8 inverted microscope (Leica, Germany) with 20x objective (dry lens, NA 0.75). Time lapse live cell images were recorded every 5s for a total time period of 5 min, and the period between 2 peaks (2 Ca 2+ oscillations) was measured in order to calculate the corresponding periods.
The concentration of afatinib varied from 5 µM to 0.25 µM. For the combination of RP4010 and afatinib, the ratio of RP4010 to afatinib was 2:1. KYSE-150 cells were loaded with 5 µM Fluo-4 in 96-well imaging plates (BD Falcon, NJ) at room temperature for 20 min. After washing, cells were kept in culture medium without phenol red. The intensity of fluorescent signals were recorded by Hamamatsu digital camera C11440 complemented with DMi8 inverted microscope (Leica, Germany) with 20x objective (dry lens, NA 0.75). Time lapse live cell images were recorded every 5s for a total time period of 5 min, and the period between 2 peaks (2 Ca 2+ oscillations) was measured in order to calculate the corresponding periods.
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