The hBMMSCs (2 × 104 cells/ well) were seeded into 24 well plates and stimulated to differentiate along the osteoblastic lineage using (StemPro ®) osteoblast differentiation kit (A10072-01, ThermoFisher Scientific). The control cells were cultured using the differentiation basal medium alone while the cells stimulated to undergo osteoblastic differentiation were cultured in basal medium fortified with osteogenic supplement (StemPro®) for up to 21 days with fresh media change every 3–4 days. Following differentiation, the cells were fixed in 4% formaldehyde solution for 30 min, rinsed twice with PBS and stained with Alizarin red (Sigma) solution, washed and analyzed by light microscopy.
Stempro osteoblast differentiation kit
Manufactured by Thermo Fisher Scientific
The StemPro® osteoblast differentiation kit is a laboratory product designed to promote the differentiation of stem cells into osteoblasts, which are cells responsible for bone formation. The kit provides a defined, serum-free culture system that supports the directed differentiation of stem cells towards the osteoblast lineage.
Automatically generated - may contain errors
Lab products found in correlation
Alizarin red, by Merck Group (2 mentions)
Alizarin red, by ScienCell (2 mentions)
Hmscs, by Lonza (2 mentions)
Masson s trichrome stain, by Merck Group (1 mentions)
Zinc pyrithione, by Merck Group (1 mentions)
Fluozin 3, by Thermo Fisher Scientific (1 mentions)
Masson trichrome, by Merck Group (1 mentions)
Safranin o, by Merck Group (1 mentions)
Pcdna6.2 v5 dest, by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
4 protocols using stempro osteoblast differentiation kit
1
Osteoblastic Differentiation of hBMMSCs
2
Osteoblastic Differentiation of BM-MSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The BM-MSCs (5 × 104 cells/well) were seeded into a 6-well plate and differentiated along the osteoblastic lineage using StemPro® osteoblast differentiation kit (A10072-01, Thermo Fisher Scientific). The basal differentiation medium was fortified with osteogenic supplement (StemPro®) and the cells were cultured for up to 21 days with fresh medium every 72 h. The differentiated cells were then fixed in 4% formaldehyde solution for 30 min, stained with Alizarin red (Sigma) solution, and analyzed for positive staining using light microscopy.
3
Differentiation of hMSCs into Fibrogenic and Osteogenic Lineages
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
hMSCs (Lonza, Basel, Switzerland) were cultured at 37 °C in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS). Fibrogenic differentiation was induced by connective tissue growth factor (CTGF) treatment [16 (link)]. Osteogenic differentiation was induced by using a StemPro® Osteoblast Differentiation Kit (Gibco). Masson’s trichrome stain (Sigma, St. Louis, MO, USA) and Alizarin red (ScienCell Research Laboratories, Carlsbad, CA, USA) were used as previously described [16 (link)].
4
Zinc Homeostasis in Mesenchymal Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell culture and materials hMSCs were purchased (Lonza, Basel, Switzerland) and maintained in DMEM medium (Gibco, Carlsbad, CA) with 10% fetal bovine serum and antibiotics, at 37 C. siRNA transfection was performed with ON-TARGETplus SMARTpool siRNA (Life Technologies, Carlsbad, CA) using Lipofectamine RNAimax (Life Technologies) according to the manufacturer's instructions. Fluozin-3 (Life Technologies) and zinc pyrithione (Sigma, St. Louis, MO) were dissolved in DMSO and stocked at À80 C before use. Fibrogenic differentiation was induced by the connective-tissue growth factor treatment (Lee et al., 2010) . Osteogenic differentiation was induced by a StemPro Osteoblast Differentiation Kit (Gibco). Masson Trichrome (Sigma), Alizarin red (Sciencell Research Laboratories, Carlsbad, CA), and Safranin O (Sigma) were used to stain differentiated cells. To construct V5-tagged ZIP7, mouse cDNA was ligated into pcDNA6.2/V5-DEST (Life Technologies) as previously described (Bin et al., 2011) .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!