STX-S or 293F parental exosomes were mixed with anti-CD81-labeled magnetic beads for 2 h at RT (catalog number 10622D; Thermo Fisher) and washed twice with PBS using a magnetic stand. Next, the bead-exosome mixtures were incubated with either directly Alexa Fluor 647-conjugated antispike antibody (see the section on flow cytometry, above), fluorescein isothiocyanate (FITC)-conjugated anti-CD81 antibody (catalog number 551108; BD Biosciences), or FITC-conjugated mouse IgGκ isotype control antibody (catalog number 555748; BD Biosciences) for 1 h at RT, followed by two PBS washes. 293F exosomes were used as a negative control for spike expression, and the isotype antibody was used as a negative control for CD81 expression. Samples were analyzed on a CytoFlex S flow cytometer (Beckman Coulter), and data were analyzed by using FlowJo (Table S2).
Fitc conjugated mouse iggκ isotype control antibody
Manufactured by BD
The FITC-conjugated mouse IgGκ isotype control antibody is a laboratory reagent used as a control in flow cytometry and other immunoassays. It is a fluorescently labeled mouse immunoglobulin G (IgG) antibody that binds to κ light chains, serving as a negative control to measure non-specific binding.
Automatically generated - may contain errors
2 protocols using fitc conjugated mouse iggκ isotype control antibody
1
Exosome Surface Protein Analysis
2
Exosome Surface Protein Analysis
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STX-S or 293F parental exosomes were mixed with anti-CD81-labeled magnetic beads for 2 h at RT (catalog number 10622D; Thermo Fisher) and washed twice with PBS using a magnetic stand. Next, the bead-exosome mixtures were incubated with either directly Alexa Fluor 647-conjugated antispike antibody (see the section on flow cytometry, above), fluorescein isothiocyanate (FITC)-conjugated anti-CD81 antibody (catalog number 551108; BD Biosciences), or FITC-conjugated mouse IgGκ isotype control antibody (catalog number 555748; BD Biosciences) for 1 h at RT, followed by two PBS washes. 293F exosomes were used as a negative control for spike expression, and the isotype antibody was used as a negative control for CD81 expression. Samples were analyzed on a CytoFlex S flow cytometer (Beckman Coulter), and data were analyzed by using FlowJo (Table S2).
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