Ammonium hydrogen carbonate nh4hco3

Keggin-type phosphomolybdic acid H₃PMo₁₂O₄₀^.xH₂O (PMo₁₂), sodium dodecyl sulfate (SDS), cesium carbonate (Cs₂CO₃), Polyvinylpyrrolidone (PVP K30, average Mw: 300 000) were obtained from Alfa Aesar. Cyt-C (30398, 95%), Hb (H2625, 95%), BSA (A3311, 95%) proteins, and ammonium hydrogen carbonate (NH₄HCO₃) were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used without further purification. Sodium citrate, citric acid, acetonitrile, isopropyl alcohol, acetone, ethyl alcohol, and guanidine hydrochloride were acquired from Sinopharm Chemical Reagent (Shanghai, China). Radio immunoprecipitation assay lysis buffer (RIPA) was purchased form Beyotime technology. Macro spin column TARGA C18 was purchased from The Nest Group, Protein LoBind tubes and ultrafiltration tubes (10 kDa) were purchased from Eppendorf. DL-dithiothreitol (DTT) and iodoacetamide (IAA) were supplied by Acros Organics. Sequencing grade modified trypsin (Promega), formic acid (FA), acetonitrile (ACN), methanol, acetic acid, and Pierce BCA protein assay kit were purchased from Thermo Fisher Scientific. Deionized water (ρ = 18 MΏ cm, 25 °C) was obtained from a Millipore Milli-Q water purification system.

The following chemicals and reagents were used: liensinine alkaloid was purchased from Tianjin Shilan Tech., China. The internal standard (IS) dauricine was purchased from Beijing Beina Chuanglian Biotechnology Institute (Beijing, China). HPLC-grade methanol and acetonitrile (ACN) were purchased from Thermo Fisher Scientific (Pittsburgh, PA, USA). Mass-grade formic acid and ammonium hydrogen carbonate (NH₄HCO₃) were purchased from Sigma-Aldrich (Switzerland). MTBE was purchased from Shanghai Richjoint Chemical Reagents (Shanghai, China). Water was produced using a MK-459 Millipore Milli-Q Plus ultrapure water system. Plasma was separated from blood collected by retro-orbital bleeding of rats.