Antibodies anti-CD14-FITC, anti-CD16-APC, anti-CD56-PE, anti-CD3-APC, anti-CD4-FITC, anti-CD8-PE, and anti-CD19-PE were purchased from BD Biosciences (Heidelberg, Germany). The Luminex assay kit was purchased from Bio-Rad company (Hercules, CA, USA).
Luminex assay kit
Manufactured by Bio-Rad
Sourced in United States
The Luminex assay kit is a multiplex analysis platform that allows simultaneous detection and quantification of multiple analytes from a single sample. The core function of the kit is to provide a flexible, scalable, and high-throughput solution for various applications, including protein profiling, cytokine/chemokine analysis, and biomarker detection.
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Lab products found in correlation
4 protocols using luminex assay kit
1
Multiparametric Flow Cytometry Analysis
2
Cytokine Protein Analysis via Luminex
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Supernatants were collected from the siRNA experiments prior to cell harvest. Thereafter, cytokine protein levels were measured using a luminex assay kit according to the manufacturer’s protocol (Bio-rad, South Africa).
3
Cytokine Analysis of Cultured Cells
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Supernatants from cells cultured for 24 h under various conditions (medium alone, or medium plus DMOG, TNF-α, or DMOG + TNF-α) were collected, and cytokine analysis performed using a Luminex assay kit (Bio-Rad, Hercules, CA) according to the manufacturer’s protocol. The following growth factors/cytokines were measured: VEGF, IL-6, IL-8, MMP-9, MCP-1, MMP-2, IL-4, IL-10, and IL-1β. Samples were measured in duplicate.
4
Plasma Biomarkers of Inflammation
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The levels of high mobility group box 1 (HMGB1) and myeloperoxidase (MPO) in the plasma were measured by ELISA according to the manufacturer’s instructions by using HMGB1 kit (IBL International GmbH, Hamburg, Germany) and MPO kit (Hycult Biotech, Uden, Netherlands), respectively. The respective levels of proinflammatory cytokine (interleukin 6 (IL-6), IL-8, tumor necrosis factor α (TNF-α), monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory proteins-1β (MIP-1β), and granulocyte-colony stimulating factor (G-CSF)) and anti-inflammatory cytokine (IL-5, L-10, and IL-13) present in the plasma samples were analyzed using a Luminex assay kit (Bio-Rad Laboratories, Hercules, CA).
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