Luck 10g

Six- to 8-week-old male NOD.Cg-Rag1^tm1Mom Il2rg^tm1Wjl/SzJ (NRG, The Jackson Laboratory) mice were irradiated with a sublethal dose of 200  cGy (Rad-Source RS-2000) and injected on the same day with the indicated numbers of A673-Fluc cells. For the metastatic ES model tumor cells were injected through the lateral tail vein. For the orthotopic model, tumor cells were injected intratibially. On day 7 after tumor cell injection, the indicated number of LINGO1 CAR T cells or CAR T cells lacking a binding domain (ΔscFv) were injected into the lateral tail vein. To determine CAR T cell efficacy, animals were weighed twice weekly and monitored for signs of distress in accordance with institutional regulations. For in vivo imagining, mice received an intraperitoneal injection of 3.3  mg D-luciferin (GOLDBIO # LUCK-10G). Photographic and luminescent images were acquired starting 10 minutes after the D-luciferin injection, both in prone and supine position using an in vivo imaging system (IVIS). ES progression was monitored every 7 days until the study endpoint. Average radiance (p/s/cm²/sr) was quantified for individual animals using Living Image 4.8 software (PerkinElmer).