The following fluorophore-conjugated antibodies were used for BCC lines and NK cell staining: anti-hCD321 (JAM-A; clone OV-5B8) were derived from BioLegend (San Diego, California, USA). anti-hMICA (clone 159227), anti-hMICB (clone 236511), anti-ULBP1 (clone 170818), anti-ULBP2/5/6 (clone 165903), anti-ULBP3 (clone 166510), anti-CD3 (clone SK7), anti-CD56 (clone CMSSB), anti-CD16 (clone CB16), anti-CD69 (clone FN50), anti-NKG2D (clone 1D11), anti-NKp46 (clone 9E2), anti-PD-1 (clone MIH4), and anti-CD62L (clone DREG56) were derived from Invitrogen (Waltham, Massachusetts, USA); anti-NKG2A (clone 131411) and anti-NKG2C (clone 134591) were derived from R&D systems (Minneapolis, Minnesota, USA); anti-DNAM-1 (DX11), and anti-CD107a (clone H4A3) were derived from BD bioscience (San Jose, CA, USA). The Live/Dead Fixable violet dead cell stain kit (Invitrogen) was used to determine the NK cell viability. The cells were then stained with antibodies for 30 min on ice. Flow cytometry was performed using a FACSVerse or FACSLyric (BD Biosciences). Flow cytometry data were analyzed using the Kaluza software (Beckman Coulter Inc., Brea, California, USA).
Anti nkg2c clone 134591
Manufactured by R&D Systems
Sourced in United States
Anti-NKG2C (clone 134591) is a mouse monoclonal antibody that recognizes the NKG2C receptor expressed on natural killer (NK) cells and a subset of T cells. It can be used for the detection and quantification of NKG2C-positive cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd107a clone h4a3, by BD (2 mentions)
Facslyric, by BD (1 mentions)
Live dead fixable violet dead cell stain kit, by Thermo Fisher Scientific (1 mentions)
Facsverse, by BD (1 mentions)
Kaluza software, by Beckman Coulter (1 mentions)
Anti nkg2a clone 131411, by R&D Systems (1 mentions)
Apc conjugated streptavidin, by Thermo Fisher Scientific (1 mentions)
Anti tnf α clone mab11, by BioLegend (1 mentions)
2 protocols using anti nkg2c clone 134591
1
Characterizing NK Cell Phenotype and Function
2
Comprehensive Immune Phenotyping Panel
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The following fluorescent conjugated anti-human monoclonal antibodies (mAbs) were utilized in this study: anti-CD3 (clone UCHT-1 and clone SK7), anti-CD56 (clone HCD56), anti-CD69 (clone FN50), anti-IFN-γ (clone 4S.B3) and anti-TNF-α (clone MAb11) mAbs were from Biolegend. Anti-NKp30 (clone Z25), anti-NKp44 (clone Z231), anti-NKG2A (clone Z199), anti-KIR2DL1/S1 (clone EB6B) and anti-KIR3DL1/S1 (clone Z27) mAbs were from Beckman Coulter. Anti-NKp46 (clone 9E2/NKp46), anti-KIR2DL2/L3/S2 (clone DX27), and anti-CD107a (clone H4A3) mAbs were from BD Bioscience. Anti-NKG2C (clone 134,591) mAb was from R&D system. Biotinylated anti-NKG2D (clone 1D11) mAb and APC conjugated streptavidin were from eBioscience.
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