Fatty-acid profile was established by transmethylation of triglycerides and further analysis by gas chromatography [79 (link)]. Forty (40) mg of oil were transmethylated in 900 µL of diethyl ether with 100 μL of tetramethyl ammonium hydroxide (in 25% methanol). The mix was incubated at room temperature for 10 min. Focus gas chromatography (ThermoFisher Scientific, Courtaboeuf, France) with a flame ionization detector (FID) was used for fatty-acid quantitation. Fatty-acid separation was performed using a capillary BPX-90 column (30 m × 0.25 mm × 0.25 μm; Trajan Scientific Europe Ltd., Milton Keynes, UK). One (1) μL of sample was injected at 270 °C with a split ratio of 1/14. Oven temperature was set at 170 °C for 5 min and was increased to 230 °C with a 45 °C /min slope. The temperature was maintained for 2 min. Hydrogen was used as an eluent at a flow rate of 0.7 mL/min. Detection was performed at 280 °C.
Focus gas chromatography
Manufactured by Thermo Fisher Scientific
Sourced in France
The Focus gas chromatography system from Thermo Fisher Scientific is an analytical instrument used for the separation, identification, and quantification of chemical compounds in complex mixtures. It employs gas chromatography technology to achieve these objectives.
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Lab products found in correlation
2 protocols using focus gas chromatography
1
Fatty Acid Profile Analysis by GC
2
GC-MS Analysis of Essential Oils
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Analysis of the essential oils obtained by classical hydrodistillation method was performed using a Thermo Scientific Focus Gas Chromatography equipped with a Mass Spectrometer, autosampler and TR-5MS capillary column (5% phenylpolisilfenilenesiloxan, 30 m x 0.25 mm inner diameter, film thickness 0.25). The carrier gas was helium (99.9%) with a flow rate of 1 mL/ min; ionization energy was 70 eV. Mass range m/z 50-650 amu. Data acquisition was scanning mode. Transfer line temperature of the mass spectrometer was 220°C; the temperature of orifice injection was 220°C. The samples were injected with a split ratio of 250. The injection volume was 1 mL. The temperature of oven was programmed in the range of 50 to 220°C at 3°C/min. The structure of each compound was identified by comparison of their mass spectra (Wiley 9 library). The data were processed using Xcalibur software.
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