Evo m mlv 2 reverse transcriptase

Total RNA was extracted from cells by AG RNAex Pro Reagent (Accurate Biology) according to the manufacturer’s instructions. Reverse transcription was performed with the Evo M-MLV II Reverse Transcriptase (Accurate Biology). Real-time quantitative PCR (RT-qPCR) was performed using SYBR Green Premix Pro Taq HS qPCR Kit (Accurate Biology) according to the manufacturer’s protocol. Gene expression level was normalized to GAPDH level in respective samples as an internal control, and the results were performed with at least three independent experiments. The primers used for RT-qPCR were: 5’-GGGCCTCGCTACCAATACTTG-3’ (forward) and 5’-CGGAACGTCGATCATAGTTTTCA-3’ (reverse) for B3GNT5; 5’-TGCACCACCAACTGCTTAGC-3’ (forward) and 5’-GGCATGGACTGTGGTCATGAG-3’ (reverse) for GAPDH.

Total RNA was extracted from cells by AG RNAex Pro Reagent (Accurate Biology) according to the manufacturer’s instructions. Reverse transcription was performed with the Evo M-MLV II Reverse Transcriptase (Accurate Biology). Real-time quantitative PCR (RT-qPCR) was performed using SYBR Green Premix Pro Taq HS qPCR Kit (Accurate Biology) according to the manufacturer’s protocol. Gene expression level was normalized to GAPDH level in respective samples as an internal control, and the results were performed with at least three independent experiments. The primers used for RT-qPCR were: 5’- CCCTGTTGAAGCTTGCTAGG-3’ (forward) and 5’-TGGGTACCCTTGGTGAGAAG-3’ (reverse) for AMD1; 5’-TGGACCGCACACCTTGGGACA-3’ (forward) and 5’-ACGCCCACCTCCTCCGACCT-3’ (reverse) for Sox10; 5’-GCTCCTCCGATTCCGAGG-3’ (forward) and 5’-TGTTAGAGACAATGTGT-3’ (reverse) for TCF4; 5’-GCTGCGAAGTGGAAACCATC-3’ (forward) and 5’-CCTCCTTCTGCACACATTTGAA-3’ (reverse) for cyclin D1; 5’-TGCACCACCAACTGCTTAGC-3’ (forward) and 5’-GGCATGGACTGTGGTCATGAG-3’ (reverse) for GAPDH.