Blood was collected from submandibullary or tail veins. Glycemia was measured with a glucometer (Ascensia Breeze 2, Bayer Healthcare, Leverkusen, Germany). ELISAs were used to quantify glucagon (cat. no. 10-1271-01; Mercodia, Uppsala, Sweden), insulin (10-1247-10; Mercodia), and corticosterone (ADI-900-097; Enzo Life Sciences, Farmingdale, NY). Free fatty acids were measured with FUJIFILM Wako NEFA assay (Tokyo, Japan); hydroxybutyric acid was determined with the D-3-Hydroxybutyrate Ranbut reagent (Randox, Crumlin, U.K.), and plasma lactate levels were determined with lactate determination reagents (Roche, Basel, Switzerland). These measurements were performed with a Roche Diagnostics cobas c 111 automatic analyzer.
Cobas c 111 automatic analyzer
Manufactured by Roche
The Cobas c 111 is an automatic analyzer designed for clinical chemistry testing. It is a compact, bench-top instrument that automates the analysis of various chemical and biochemical components in biological samples, such as blood or urine. The Cobas c 111 is capable of performing a wide range of routine clinical chemistry tests, providing reliable and consistent results to support clinical decision-making.
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2 protocols using cobas c 111 automatic analyzer
1
Comprehensive Metabolic Profiling of Mice
2
Comprehensive Metabolic Panel Analysis
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Serum parameters were measured on a Roche Cobas C111 automatic analyzer with proprietary Roche Glucose, ALT, AST, LDH, TG and cholesterol kits, and a Fujifilm-Wako NEFA (FFA) kit. Insulinemia was assessed with a Mouse Insulin ELISA kit (Mercodia 10-1247-01). For the HF-fed cohort only, fasting glycemia was assessed with a Roche Breeze2 glucometer directly on blood samples–obtained by tail vein incision.
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