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29 protocols using ferric nitrate nonahydrate

1

Synthesis of Graphite-Based Nanomaterials

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Pristine graphite powder
(≤20 μm, 99%), ferric nitrate nonahydrate (Fe(NO3)3·9H2O), hydrogen peroxide (H2O2), potassium permanganate (KMnO4),
and sodium nitrate (NaNO3) were purchased from Sigma-Aldrich.
Nickel nitrate hexahydrate (Ni(NO3)2·6H2O), sodium dodecyl sulfate (SDS-C12H25O4SNA)-SDFCL, and urea (CH4N2O)
were purchased from Loba Ltd. Ethanol (CH2CH2OH, 99%) and sulfuric acid (H2SO4, 98%) were
purchased from Rankem, India.
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2

Synthesis and Characterization of Graphene Oxide

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The chemical reagents include natural graphite powder (200 mesh, UniRegion Bio-Tech, Hsinchu, Taiwan), zinc nitrate hexahydrate (99%, Alfa Aesar, Haverhill, MA, USA), ferric nitrate nonahydrate (98%, Sigma-Aldrich, St. Louis, MO, USA), sodium hydroxide (95%, Nihon Shiyaku Reagent, Kyoto, Japan), sulfuric acid (99.99%, Nihon Shiyaku Reagent, Kyoto, Japan), acetone (95%, Nihon Shiyaku Reagent, Kyoto, Japan), hydrochloric acid (37%, Union Chemical, Hsinchu, Taiwan), potassium permanganate (99.3%, Union Chemical, Hsinchu, Taiwan), sodium nitrate (99.5%, Hayashi Pure Chemical Ind, Osaka, Japan), hydrogen peroxide (30%, Showa Chemical, Tokyo, Japan), and methylene blue (99%, Koch-Light Laboratories, Ltd., Haverhill, Suffolk, UK). GO was prepared via modified Hummer’s method. The purity of the chemicals used in this experiment is of analytical grade, and they were used directly without any treatment.
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3

Synthesis of Lanthanum-Doped Iron Oxide Nanoparticles

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Lanthanum(III) nitrate hexahydrate (La(NO3)3·6H2O), ferric nitrate nonahydrate (Fe(NO3)3·9H2O), sodium hydroxide (NaOH, 98%), ethylene glycol (EG, 99%) and ethanol (EtOH, 99.7%) were purchased from Sigma-Aldrich (Burlington, MA, USA). Graphene oxide (GO) was purchased from Changzhou Sixth Element Material Technology Co., Ltd. (Changzhou, China). All chemical reagents were available in analytical grade and suitable for use without further purification.
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4

Embryoid Body-Derived Erythroid Differentiation

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An embryoid body was induced using AggreWell EB formation medium (StemCell Technologies). Three days after incubation in AggreWell, the embryoid body was placed on a layer of confluent adherent OP9 cells. After microscopically confirming the wheel shape of cells co-cultured for eight days, the cells were collected using collagenase IV and trypsin/EDTA.
On day 1 of differentiation to the erythroid lineage, a single-cell suspension of eight-day-co-cultured cells was prepared in erythroid basal medium containing Stemline II, 150 μg/mL human transferrin, 50 μg/mL insulin, 90 ng/mL ferric nitrate nonahydrate, and 160 μM monothioglycerol solution (all from Sigma-Aldrich, St. Louis, MO, USA). The medium was refreshed once every 2–3 days. Cell morphology was evaluated using cytocentrifugation (Cytospin 4, Thermo Fisher Scientific; 700 rpm for 7 min) and Wright–Giemsa staining (Sigma-Aldrich).
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5

Synthesis of Photocatalytic Hydrogels

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Silver nitrate, ferric nitrate nonahydrate, polyvinylpyrrolidone, potassium hydroxide, ethanol, AAm, AA, MBAAm, BACM, ammonium persulphate, and methyl orange (MO) were purchased from Sigma Aldrich Trading Co., Ltd. (Shanghai, China). All reagents were used directly without further purification. Monocrystalline silicon was purchased from GRINM Semiconductor Materials Co., Ltd. (Beijing, China).
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6

Synthesis of Metal-Oxide Nanocomposites

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Ferric nitrate nonahydrate (Fe(NO3)3.9H2O, 99%), manganese nitrate hexahydrate (Mn(NO3)2.6H2O, 99%), ethylene glycol (98%), and sodium sulfate nonahydrate (Na2SO4.9H2O, 99.99%) were purchased from Sigma-Aldrich Company. Carbon black (Super-P®, BET 65.5 m2 g−1) was purchased from Erachem Comilog Company. Nickel foam was purchased from Changsha Lyrun Material Company (Shangsha, China).
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7

Antimicrobial Nanomaterial Synthesis and Evaluation

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Ferric nitrate nonahydrate (Fe(NO3)3·9H2O), zinc nitrate (Zn(NO3)2·6H2O), aluminum nitrate nonahydrate (Al(NO3)3·9H2O), cobalt nitrate hexahydrate (Co(NO3)2·6H2O), nickel nitrate hexahydrate (Ni(NO3)2·6H2O), zirconium oxychloride (ZrOCl2·8H2O), and sodium hydroxide were purchased from Sigma-Aldrich. All solutions and experiments were prepared and conducted by suspending the nanomaterials using deionized water (DW) and sonication with the Ultrasonic Processor at 220 V/50 Hz (Model FS/000100/T). Different media types were used in this study according to the microorganism type, such as Muller Hinton broth and Muller Hinton agar for bacterial growth, while Sabarouds Dextrose Agar (SDA) and Sabarouds Dextrose Broth (SDB) were used for fungus growth using a typical or standard antifungal treatment (cyclohexamide-growing fungi and doxycycline-standard for both Gram-positive and Gram-negative bacteria).
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8

Synthesis of Complex Metal Compounds

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Ferric nitrate nonahydrate (98%), lanthanum (III) nitrate hexahydrate (99%), citric acid (98%), nitric acid (69%), acetic acid glacial ≥ 99%, ammonium hydroxide solution (30–33%), sodium hydroxide ≥ 98%, sodium chlorite (80%), lead nitrate (99%), cadmium chloride hydrate (98%), and copper sulfate pentahydrate (98%) are bought from Sigma-Aldrich. Bromocresol green is bought from Qualikems.
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9

Synthesis of Multicomponent Metal Oxide Nanoparticles

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Zinc nitrate hexahydrate
(Alfa Aesar, 99%), bismuth nitrate pentahydrate (Sigma-Aldrich, 98%),
ferric nitrate nonahydrate (Sigma-Aldrich, ≥98%), glycine (Sigma-Aldrich,
99%), sucrose (Sigma-Aldrich, 99.5%), 5,5-dimethyl-1-pyrroline N-oxide
(Sigma-Aldrich, ≥97%, DMPO), 2,2,6,6-tetramethylpiperidine
(Sigma-Aldrich, 98%, TEMP), and methyl orange (Loba Chemie) were used
as received. The water used for synthesis and other experiments was
deionized (DI).
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10

Synthesis of Metal Oxide Nanoparticles

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Ferric nitrate
nonahydrate, (Fe(NO3)3·9H2O),
ferric chloride hexahydrate (FeCl3·6H2O),
cobalt nitrate hexahydrate (Co(NO3)2·6H2O), cobalt chloride heptahydrate (CoCl2·6H2O), cobalt sulfate heptahydrate (CoSO4·7H2O), sodium hydroxide (NaOH), hydrazine hydrate
(N2H4·7H2O), sodium chloride
(NaCl), and copper sulfate pentahydrate (CuSO4·5H2O) were purchased from Sigma–Aldrich and used as received.
Reactive blue 5 was obtained from a local dyeing factory.
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