Agilent cary 630

For measurement of the zeta potential, 0.01 g of the material was ground into powder and dispersed in 10 mL of deionized water to form a colloidal solution. The samples were allowed to stand for 5 min to let larger particles settle. An aliquot was processed by a ZatasizerNanoZS particle size analyser (Malvern Instruments Ltd Co), which automatically calculates the electrophoretic mobility of the particles and converts it to the zeta potential using the Smoluchowski equation. Contact angles were obtained using the sessile drop method with a contact angle analyser (DSA1000, KRUSS GmbH). A NewView^TM 7100 white light interferometer (ZYGO Corporation Co.) was used to acquire a 3D profile image of the surface of the K3 and 3DP bio-carriers. The white light interferometer uses a special optical configuration and short-coherence-length light sources that optimize the interaction between the reflected light from the sample and the reference beam. This process characterizes and quantifies surface roughness and other topographical features with excellent precision and accuracy. Material compositions of the materials was analyzed using an ATR spectrophotometer (Agilent Cary 630, Agilent Technologies Co).

FT-IR spectra were obtained using an Agilent Cary 630 spectrophotometer (Agilent Technologies, Santa Clara, CA, USA) equipped with a Single Bounce Diamond ATR Module for KBr and MicroLab Software plus Resolutions Pro Software (Version 5.0, Agilent Technologies). FT-IR spectra were obtained in the 350–6300 cm⁻¹ range at a resolution of 2 cm⁻¹ in transmission mode.

To obtain and analyze the vibrational spectra of the EHS and FA samples in the spectral range from 4000 to 750 cm⁻¹, an IR Fourier spectrophotometer Agilent Cary 630 (Agilent, Santa Clara, CA, USA) with a transmission attachment was used [52 (link)]. Sample preparation—solid residue after drying—for spectrum recording was carried out following the requirements [53 (link)]. For this, about 1 mg of dry residue was triturated with 400 mg of carefully ground and dried potassium bromide until uniform state and compressed for 3–5 min to obtain a disk diameter of about 13 mm to have a spectrum of suitable intensity.

The formation of silver nanoparticles was monitored by UV-Visible spectroscopy (DR 5000 spectrophotometer, Hexis Científica, Jundiaí, São Paulo, Brazil,) in the spectral region between 300 and 800 nm. The absorbance values were plotted in graphs using the Origin Pro 2019b software (OriginLab Coorporation, Northampton, MA, USA, 2019). The nanoparticle diameter and Polydispersity Index (PDI) were determined by Dynamic Light Scattering (DLS) analysis using the Zetasizer Nano Instrument (Malvern, Australia, model Nano-S). The selected nanoparticles were characterized by Scanning Electron Microscopy (SEM) (JEOL microscope, model JSM-IT200, Tokyo, Japan). The functional groups present in the silver nanoparticles were analyzed by Attenuated Total Reflection-Fourier Transform Infrared (ATR-FTIR) spectroscopy (Agilent Technologies, Agilent Cary 630, Santa Clara, CA, USA), equipped with a ZnSe-diamond composite crystal accessory. The spectra were collected over a wavenumber range from 4000–600 cm⁻¹ with a spectral resolution of <2 cm⁻¹. Agilent MicroLab PC and Origin Pro 2019b software were used for data gathering and transmittance graph plotting, respectively (OriginLab, 2019).

FTIR measurements of the films were performed using an Agilent Cary 630 FTIR spectrometer (Agilent Technologies, Danbury, CT, USA) in attenuated reflectance (ATR) mode. The FTIR spectra were recorded in the range of 4000 $–$ 600 cm⁻¹. The spectra were collected in scans at a resolution of 4 cm⁻¹ for each film. All testing films were conditioned in a constant state at 25 °C and 50% relative humidity.

The crystal structures of the obtained samples were examined by X-ray diffraction (XRD) on a D8 Advance X-ray diffractometer (Germany) using Cu Kα radiation.
The microscopic morphology of MgO was analysed by scanning electron microscopy (SEM) on a Hitachi S-4100 FE-SEM instrument (Japan) at 10 kV and transmission electron microscopy (TEM) on a Tecnai G2 F20 field emission transmission electron microscope (USA) at 200 kV.
N₂ adsorption/desorption isotherm was recorded on a Micromeritics ASAP 2020 apparatus (USA) at 77 K for calculating the specific surface area and pore size distribution by the Brunauer–Emmett–Teller (BET) and Barrett–Joyner–Halenda (BJH) methods, respectively.
The vibrational characteristics of the obtained samples were recorded by a Fourier transform spectrometer (FT-IR; Agilent Cary 630, Agilent, America) in pressed KBr pellets.
The X-ray photoelectron spectrum (XPS) of N-O-MgO was obtained on a Kratos AMICUS spectrometer (SHIMADZU, JP) using Al Kα radiation. The binding energy of O element was calibrated relative to the carbon impurity with C 1s at 285 eV.
The concentrations of MO and MB in the aqueous solutions were measured using a Shimadzu UV-2550 UV-visible spectrometer (Japan).

This research used the following tools and materials: Fourier transform infrared spectrophotometer with zinc selenium as a beam splitter and a transmission module for a potassium bromide pellet holder (Agilent-Cary 630, Yishun, Singapore), personal computer (Lenovo, Beijing, China), printer (Canon, Tokyo, Japan), analytical balance (Precisa-LX 320, Dietikon, Switzerland), pellet press (Specac-Hanheld, Orpington, United Kingdom), porcelain mortar and pestle (Merck, Jakarta, Indonesia), agate mortar and pestle (Merck, Jakarta, Indonesia), glassware (Iwaki, Jakarta, Indonesia), Microlab (Agilent–OQ, PC, Quant, Lite, Yishun, Singapore), statistical package for the social sciences (International Business Machines Corporation, Selangor, Malaysia), tranexamic acid (Merck, Jakarta, Indonesia), potassium bromide (Merck, Jakarta, Indonesia), Transamin^® 500 mg tablet (Otto Pharmaceutical Industries, Bandung, Indonesia), Plasminex^® 500 mg tablet (Sanbe Farma, Bandung, Indonesia), Pytramic^® 500 mg tablet (Pyridam Farma, Cianjur, Indonesia), Kalnex^® 500 mg tablet (Kalbe Farma, Jakarta, Indonesia), Nexa^® 500 mg tablet (Sanbe Farma, Bandung, Indonesia), Nexitra^® 500 mg tablet (Ifars, Solo, Indonesia), tranexamic acid 500 mg tablet (First Medifarma, Sidoarjo, Indonesia), and tranexamic acid 500 mg tablet (Bernofarm, Sidoarjo, Indonesia).