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Stabilized peroxidase conjugated secondary antibodies

Manufactured by Thermo Fisher Scientific
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Stabilized peroxidase-conjugated secondary antibodies are laboratory reagents used in various immunoassay techniques. They consist of secondary antibodies that are conjugated to the enzyme peroxidase, which enables signal amplification and detection. These antibodies are stabilized for improved performance and storage.

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Lab products found in correlation

Anti ha, by Merck Group (2 mentions) Anti flag, by Merck Group (2 mentions) Anti myc, by Cell Signaling Technology (2 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) Anti actin, by Merck Group (2 mentions) Anti gapdh, by Merck Group (2 mentions) Anti puromycin, by Merck Group (2 mentions) Anti gw182, by Fortis Life Sciences (2 mentions) Anti dicer, by Merck Group (1 mentions)

2 protocols using stabilized peroxidase conjugated secondary antibodies

1

Characterization of Ago1x Antibody Specificity

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Polyclonal anti‐Ago1x antibody was generated in rabbit against the unique peptide sequence, RQNAVTSLDRRKLSKP, and was affinity‐purified (Pierce Biotechnology). To confirm the specificity, the anti‐Ago1x antibody was incubated with the same peptide at a final concentration of 1 μg/ml for 2 h with gentle agitation at room temperature before using it for Western blot (described below). Anti‐Ago1 antibody (Cell Signaling Technologies, 9388; Sigma, SAB4200065), anti‐Dicer (Sigma, SAB4200087), anti‐HA (Sigma, clone 3F10, 11867423001; and Cell Signaling Technology, clone 6E2, 2367), anti‐GW182 (Bethyl Laboratories, A302‐329A), anti‐puromycin (Merck Millipore, clone 12D10, MABE343), anti‐Myc (Cell Signaling Technologies, 22725), anti‐FLAG (Sigma, clone M2, F1804), anti‐Actin (Sigma, A3854), anti‐GAPDH (Sigma, G9295), and stabilized peroxidase‐conjugated secondary antibodies (Thermo Fisher Scientific) were used at the manufacturer‐recommended dilutions. Alexa Fluor‐conjugated secondary antibodies (Molecular Probes) were used for immunofluorescence.
Singh A., Manjunath L.E., Kundu P., Sahoo S., Das A., Suma H.R., Fox P.L, & Eswarappa S.M. (2019). Let‐7a‐regulated translational readthrough of mammalian AGO1 generates a microRNA pathway inhibitor. The EMBO Journal, 38(16), e100727.
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2

Characterization of Anti-Ago1x Antibody

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polyclonal anti-Ago1x antibody was generated in rabbit against the unique peptide sequence, RQNAVTSLDRRKLSKP, and was affinity-purified (Pierce Biotechnology). To confirm the specificity, the anti-Ago1x antibody was incubated with the same peptide at a final concentration of 1 μg/ml for 2 h with gentle agitation at room temperature before using it for Western blot (described below). Anti-Ago1 antibody (Cell Signaling Technologies, 9388; Sigma, SAB4200065), anti-Dicer (Sigma, SAB4200087), anti-HA (Sigma, clone 3F10, 11867423001; and Cell Signaling Technology, clone 6E2, 2367), anti-GW182 (Bethyl Laboratories, A302-329A), anti-puromycin (Merck Millipore, clone 12D10, MABE343), anti-Myc (Cell Signaling Technologies, 22725), anti-FLAG (Sigma, clone M2, F1804), anti-Actin (Sigma, A3854), anti-GAPDH (Sigma, G9295), and stabilized peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific) were used at the manufacturer-recommended dilutions. Alexa Fluor-conjugated secondary antibodies (Molecular Probes) were used for immunofluorescence.
Singh A., Manjunath L.E., Kundu P., Sahoo S., Das A., Suma H.R., Fox P.L, & Eswarappa S.M. (2019). Let‐7a‐regulated translational readthrough of mammalian AGO1 generates a microRNA pathway inhibitor. The EMBO Journal, 38(16), e100727.
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