The surface and inner morphology of the microcapsules were observed by scanning electron microscopy (SEM). Microcapsules were cross-sectioned to scan the inner structure, following a modified version of the method described by Kim et al. [25 ]. In short, representative samples of the MC formulations were dispersed in a small amount of epoxy resin (Reagent A; 5 Minute® Epoxy, Devcon®, Solon, OH, USA) until a homogeneous suspension was formed. An equal amount of catalyst (reagent B) was added, and both components were mixed for 1 min to start the polymerization reaction. The mixture was allowed to stand for 2 h to allow the resin to harden. The resulting pellet was cross-sectioned with a razor blade, making several random cuts, thus sectioning some of the MCs embedded in the epoxy matrix. Samples of whole and cross-sectioned MCs were mounted on aluminum stubs covered with double-sided, adhesive, electrically conductive carbon tape and sputter-coated with gold/palladium (SDC 005 Sputter Coater, BAL-TEC GmbH, Schalksmühle, Germany). Afterwards, SEM micrographs were taken using a JEOL JSM-6360LV scanning electron microscope (JEOL Ltd., Tokyo, Japan) at an accelerating voltage of 15 kV and appropriate magnification.
Jsm 6360lv scanning electron microscope
Manufactured by JEOL
Sourced in Japan, United States
The JSM-6360LV is a scanning electron microscope (SEM) manufactured by JEOL. It is designed to provide high-resolution imaging and analysis of a wide range of samples. The JSM-6360LV features a low vacuum mode, which allows the observation of non-conductive samples without the need for complex sample preparation. The instrument is equipped with a tungsten electron source and can achieve a resolution of up to 3.0 nm.
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Lab products found in correlation
Jem 1230 transmission electron microscope, by JEOL (3 mentions)
Jfc 1600 auto fine coater, by JEOL (2 mentions)
0.1 m cacodylate buffer, by Merck Group (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
X pert, by Philips (1 mentions)
Jem 2000ex, by JEOL (1 mentions)
Sz61 stereomicroscope, by Olympus (1 mentions)
Axioscope microscope, by Zeiss (1 mentions)
1200ex microscope, by JEOL (1 mentions)
E200mv, by Nikon (1 mentions)
Smz745t, by Nikon (1 mentions)
Isomet 2000 precision saw, by Buehler (1 mentions)
Sp2 laser scanning confocal microscope, by Leica (1 mentions)
Axiophot, by Zeiss (1 mentions)
Vhx 1000 digital microscope, by Keyence (1 mentions)
Dm5500b microscope, by Leica (1 mentions)
Em cpd300 automated critical point dryer, by Leica (1 mentions)
Dfc550 digital camera, by Leica (1 mentions)
Imprint 2 garant, by 3M (1 mentions)
Poly l lysine coated slide, by Merck Group (1 mentions)
34 protocols using jsm 6360lv scanning electron microscope
1
Microcapsule Morphology Analysis by SEM
2
Yeast Cell Adhesion Visualized by SEM
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To examine yeast cell adhesion by SEM, 2 mL of the standardized cell suspension (1 x
107 yeast/mL in RPMI 1640) was added to the wells of 24-well
polystyrene plate and incubated for 2 h at 37 ºC in a shaker at 120 rev/min. After 2
h, the medium was aspirated, and non-adherent cells were removed by washing twice
with PBS. Samples were fixed with 2.5% glutaraldehyde diluted in 0.1 M cacodylate
buffer (Sigma, St. Louis, Missouri, USA) and dehydrated with an ascending series of
alcohol solutions. Samples were kept in a desiccator until the bases of the wells
were removed for analysis. Prior to observation, the bases of the wells were mounted
onto aluminum stubs, sputter coated with gold and observed with a JEOL-JSM 6360 LV
scanning electron microscope (Jeol Ltd., Tokyo, Japan) at the Electron Microscopy
Center, State University of Paraná, Maringá, Brazil.
107 yeast/mL in RPMI 1640) was added to the wells of 24-well
polystyrene plate and incubated for 2 h at 37 ºC in a shaker at 120 rev/min. After 2
h, the medium was aspirated, and non-adherent cells were removed by washing twice
with PBS. Samples were fixed with 2.5% glutaraldehyde diluted in 0.1 M cacodylate
buffer (Sigma, St. Louis, Missouri, USA) and dehydrated with an ascending series of
alcohol solutions. Samples were kept in a desiccator until the bases of the wells
were removed for analysis. Prior to observation, the bases of the wells were mounted
onto aluminum stubs, sputter coated with gold and observed with a JEOL-JSM 6360 LV
scanning electron microscope (Jeol Ltd., Tokyo, Japan) at the Electron Microscopy
Center, State University of Paraná, Maringá, Brazil.
3
Scanning Electron Microscopy of RBL-2H3 Cells
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Previously plated on glass coverslips, RBL-2H3 cells were exposed to PBS (as negative control) and LiRecTCTP (100 and 200 µg/mL) in Tyrode’s Buffer (TGB) for 2 h at 37 °C. After, cells were washed with TGB and fixed with Karnovsky (2% formaldehyde, 2.5% glutaraldehyde in 0.1 M of sodium cacodylate buffer pH 7.2 at 4 °C) [18 ]. Then, fixed cells were dehydrated, and critical-point drying was performed using a Balzers CPD-010 (Balzers Instruments, Balzers, Liechtenstein) with carbonic gas. Metallization in gold was performed using a Balzers SCD-030 (Balzers Instruments). The samples were observed and photographed with a JEOL-JSM 6360 LV scanning electron microscope (JEOL Ltd., Tokyo, Japan) at the Electron Microscopy Center, Federal University of Paraná (Curitiba, PR, Brazil).
4
SEM Imaging of Cell Morphology
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For SEM, 105 cells were fixed on microscope slides for 1 h in Karnovski solution (2% glutaraldehyde, 4% paraformaldehyde, 1 mM CaCl2 in 0.1 M sodium cacodylate buffer), and post-fixed with 1% osmium tetroxide, for 1 h. The cells were dehydrated and then subjected to the critical point in the CPD 010 (Critical Point Dryer), plated with gold in the apparatus SCD 030 (Balzers Union, Liechtenstein). Images were taken under a JEOL JSM 6360–LV Scanning Electron Microscope (Jeol USA Inc., Peabody, MA, USA) [63 (link)]. 3 independent experiments were performed in duplicates.
5
Anther Development and Pollen Viability Analysis
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Photographs of the fresh anthers at each sequential stage were obtained from XN1376 and XN1376-CIMS with a Nikon E995 digital camera (Nikon, Tokyo, Japan) fixed firmly to a Motic K400 dissecting microscope (Preiser Scientific, Louisville, KY, United States). Pollen grains were also analyzed via 1% iodine–potassium iodide (1% KI–I2) staining to determine the viability of the mature pollen, as previously described [65 (link)]. Scanning electron microscopy was used to characterize the surface characteristics of the anthers. Before the experiment, fresh anthers and pollen grains were fixed in 4% glutaraldehyde and then treated with an alcohol gradient, dried, and broken in sequence. Finally, the anthers and pollen grains were mounted on a stub with colloidal silver and photographed using a JSM-6360LV scanning electron microscope (JEOL, Tokyo, Japan) [66 (link)].
6
SEM Analysis of Spore Morphology
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For SEM analysis, mature spores from different populations were dispersed on stubs directly after being collected. The spores were gold-coated in a JFC-1600 Auto Fine Coater and observed using a JEOL JSM-6360LV Scanning Electron Microscope at 25 kV at the South China Botanical Garden, Chinese Academy of Sciences. The spore mean sizes of 7 populations of A. reniforme var. sinense and 7 populations of A. reniforme var. reniforme were measured by Smile View software (20 spores per population), and a scatter diagram was made with SPSS. The descriptive terminology in Spores of Polypodiales (Filicales) from China [11 ] and Plant identification terminology: An illustrated glossary [37 ] was followed.
7
Characterization of Agronomic Traits in F1 Hybrids
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The agronomic traits of F1 individuals were measured on five plants randomly selected from the reciprocal crosses, and all measurements were done according to [26 (link)]. Plant height was measured from the ground to the top of the spike, and the spike number per plant, spikelet number on the main stem spike, seed number on the main stem spike, seed number per spikelet of the main stem, and thousand seed weight were investigated. The mean values were used to characterize the corresponding traits. Photographs of F1 spikes were obtained using a Nikon D600 digital camera (Nikon, Tokyo, Japan), and photographs of pistils were obtained using a Nikon E995 digital camera (Nikon) mounted on a Motic K400 dissecting microscope (Preiser Scientific, Louisville, KY, USA). For cytological examination, young spikes were processed as described by [27 (link)] and observed with a JSM-6360LV scanning electron microscope (JEOL, Tokyo, Japan).
8
Thoracic Aorta Histological Analysis
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After fixed with 4% paraformaldehyde, thoracic aortas were embedded with paraffin, and paraffin section (5 mm) was stained using hematoxylin and eosin. In scanning electron microscopic observation, after cut open and fixed with 3% glutaraldehyde, the thoracic aortas were incubated with 2% OsO 4 and dehydrated in ethanol. The aortas were then critical point dried, sputter-coated with gold, and observed using a JSM-6360LV scanning electron microscope (JEOL, Tokyo, Japan).
9
Scanning Electron Microscopy of Fixed Cells
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The strains were fixed in 2.5% glutaraldehyde for 48 h, rinsed three times in phosphate buffer (0.2 mol/L), and dehydrated in an ethanol series (30%, 50%, 70%, 90%, and 100%). Ethanol was displaced by isoamyl acetate, and the cells were dried using an EMS 850 critical point drying apparatus. Next, the samples were mounted on microscope slides (approximately 2.5 cm×2.5 cm) and sputter-coated with gold to a thickness of approximately 20 nm. Finally, they were observed under a JSM-6360LV scanning electron microscope (JEOL, Tokyo, Japan) operating at 30 kV.
10
SEM Analysis of Optimized SLNs
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The surface morphology of optimized SLNs (F2) was studied using SEM (JSM-6360LV Scanning Electron Microscope, Jeol, Tokyo, Japan). The suspension of SLNs (F2) was vortexed for 3 min, spread as a film on glass slide, and air dried and scanned for imaging [25 (link)].
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