Acetonitrile 99.9% of HPLC grade was from Fisher Scientific (Lisbon, Portugal). Ellipticine was purchased from Sigma-Aldrich (St. Louis, MO, USA), as also were acetic acid, sulforhodamine B (SRB), trypan blue, trichloroacetic acid (TCA), and Tris-(hydroxymethyl)aminomethan (TRIS). Phenolic compound standards were from Extrasynthèse (Genay, France). RPMI-1640 medium, fetal bovine serum (FBS), Hank’s balanced salt solution (HBSS), l -glutamine, nonessential amino acids solution (2 mM), penicillin/streptomycin solution (100 U/mL and 100 mg/mL, respectively), and trypsin-EDTA (ethylenediaminetetraacetic acid) were from Hyclone (Logan, UT, USA). All other chemicals were of analytical purity and obtained from common suppliers. Water was treated via the purification system Milli-Q water (TGI Pure Water Systems, Greenville, SC, USA).
Phenolic compound standards
Manufactured by Extrasynthese
Sourced in France
Phenolic compound standards are reference materials used for the identification and quantification of phenolic compounds in various analytical techniques. These standards provide a known concentration and composition of specific phenolic compounds, which are essential for calibrating analytical instruments and verifying the accuracy of analytical results.
Automatically generated - may contain errors
Lab products found in correlation
2 2 diphenyl 1 picrylhydrazyl (dpph), by Thermo Fisher Scientific (8 mentions)
Ellipticine, by Merck Group (7 mentions)
Acetic acid, by Merck Group (6 mentions)
Trypan blue, by Merck Group (5 mentions)
Formic acid, by Merck Group (5 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Merck Group (5 mentions)
Trichloroacetic acid, by Merck Group (4 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (4 mentions)
Ethyl acetate, by Thermo Fisher Scientific (4 mentions)
Sulforhodamine b, by Merck Group (3 mentions)
Trichloroacetic acid tca, by Merck Group (3 mentions)
Hplc grade acetonitrile, by Merck Group (3 mentions)
Trolox, by Merck Group (3 mentions)
Tryptic soy broth (tsb), by bioMérieux (3 mentions)
P iodonitrotetrazolium chloride int, by Merck Group (3 mentions)
Acetonitrile, by Thermo Fisher Scientific (3 mentions)
N hexane, by Thermo Fisher Scientific (3 mentions)
Acetonitrile 99.9 of hplc grade, by Thermo Fisher Scientific (2 mentions)
Silica gel 0.060 0 200 mm 60 a, by Thermo Fisher Scientific (2 mentions)
Sulforhodamine b srb, by Merck Group (2 mentions)
18 protocols using phenolic compound standards
1
Acetonitrile-based Bioassay Protocol
2
Analytical Reagents for Cell Culture Studies
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Acetonitrile 99.9% was of HPLC grade was obtained from Fisher Scientific (Lisbon, Portugal). Phenolic compound standards were obtained from Extrasynthese (Genay, France). Formic acid was obtained from Sigma-Aldrich (St. Louis, MO, USA). Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA). Fetal Bovine Serum (FBS), solutions of penicillin (100 IU/mL) and streptomycin (10 mg/mL), RPMI-1640 medium, trypsin-EDTA (ethylenediaminetetraAcetic acid), L-glutamine and Hank’s Balanced Salt Solution (HBSS) were purchased from Hyclone (Logan, USA). Acetic acid, sulforhodamine B (SRB), trypan blue, trichloroAcetic acid (TCA) and Tris-base were purchased from Sigma Chemical Co. (Saint Louis, USA). Silica gel 0.060–0.200 mm 60 A was obtained from Acros Organics (Geel, Belgium).
3
Quantification of Phytochemicals in Samples
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HPLC-grade acetonitrile was obtained from Merck KgaA (Darmstadt, Germany). Standards of melezitose, fatty acids methyl ester (FAME, reference standard mixture 3747885-U), Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), and 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH) were purchased from Sigma Chemical Co. (St. Louis, MO, USA); tocol standard (50 mg/mL) was acquired from Matreya, Pleasant Gap (PA, USA), and the phenolic compound standards were acquired from Extrasynthese (Genay, France). Ethanol and all other chemicals and solvents were of analytical grade and purchased from common sources. Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA)
4
Chromatographic Analysis of Biochemicals
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High-performance liquid chromatography (HPLC)-grade acetonitrile (99.9%) was acquired from Fisher Scientific (Lisbon, Portugal). Cesium chloride and lanthanum chloride were acquired from Thermo Fisher Scientific (Waltham, MS, USA). Phosphate buffered saline (PBS), trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), thiobarbituric acid (TBA), trichloroacetic acid, 2.2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH), streptomycin, ellipticine, sulforhodamine B, lipopolysaccharide (LPS), dexamethasone, the fatty acids methyl ester (FAME) standard 47885-U, and the sugar and organic acid standards were purchased from Sigma-Aldrich (St. Louis, MO, USA). The phenolic compound standards were purchased from Extrasynthèse (Genay, France). Ampicillin, bifonazole, ketoconazole, and p-iodonitrotetrazolium violet were acquired from Panfarma (Belgrade, Serbia), Srbolek (Belgrade, Serbia), Zorkapharma (Šabac, Serbia), and Biochemica (Panreac, Barcelona, Spain), respectively. Further chemicals were supplied by common sources.
5
Antioxidant Capacity Evaluation of Phenolic Compounds
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HPLC-grade acetonitrile (99.9%) was obtained from Fisher Scientific (Lisbon, Portugal) and formic acid from Sigma-Aldrich (St. Louis, MO, USA). The phenolic compound standards were from Extrasynthese (Genay, France). Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA). 2,2-diphenyl-1-picrylhydrazyl (DPPH) was obtained from Alfa Aesar (Ward Hill, MA, USA). Standard trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) was from Sigma (St. Louis, MO, USA). RAW 264.7 cells were purchased from ECACC (“European Collection of Animal Cell Culture”) (Salisbury, UK) and DMEM from Hyclone (Logan, Utah, US). The Griess Reagent System Kit was purchased from Promega (Madison, WI, USA). Dulbecco's modified Eagle's medium (DMEM), hank's balanced salt solution (HBSS), fetal bovine serum (FBS), L-glutamine, trypsin-EDTA, penicillin/streptomycin solution (100 U/mL and 100 mg/mL, respectively) were purchased from Gibco Invitrogen Life Technologies (California, USA). Methanol and all other chemicals were of analytical grade and obtained from local suppliers.
6
Comprehensive Chemical Characterization
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All chemicals were obtained as pure commercial products and used without further purification. Standards of fatty acids and fatty acid methyl esters, Desferal (deferoxamine mesylate salt), Trolox, Folin-Ciocalteau's phenol reagent, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), allopurinol, xanthine oxidase from cow's milk (XO), acetylcholinesterase (AChE) from Electrophorus electricus, butyrylcholinesterase (BChE) from equine serum, xanthine, 5,5′-dithiobis-(2-nitrobenzoic) acid (DTNB), acetylthiocholine iodide (ATCI), S-butyrylthiocholine iodide (BTCI), (−)-epicatechin-(4β,8)-(+)-catechin (procyanidin B1) and all solvents used, of the highest available purity, were obtained from Sigma-Aldrich (Milan, Italy). Methanolic HCl (3 N) was purchased from Supelco (Bellefonte, PA). The phenolic compound standards were obtained from Extrasynthese (Genay, France). HPLC-grade acetonitrile was obtained from Merck KgaA (Darmstadt, Germany), and formic acid was purchased from Prolabo (VWR International, France). Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, USA).
7
In Vitro Digestive Enzyme Inhibition
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Porcine pancreatic α-amylase (type IV-B), pancreatic lipase (type II) and potato starch were purchased from Sigma-Aldrich. cyanidin-3-O-glucoside hydrochloride (C3G, 98% pure) was purchased from Biopurify Phytochemicals Ltd (Chengdu, China). Phenolic compound standards (ellagic acid, quercetin-3-O-glucoside and cyanidin-3-O-glucoside) were purchased from Extrasynthese (Genay, France). Excipient-free acarbose and orlistat were obtained from Manipulação Farmácias São Paulo (Maringá, Brazil). Origin of both products is Fagron SM (Waregem, Belgium). All reagent grade chemicals were from the highest possible degree of purity.
8
Phenolic Compounds Purification and Evaluation
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HPLC-grade acetonitrile was obtained from Merck KgaA (Darmstadt, Germany).
Formic and Acetic acids were purchased from Prolabo (VWR International, France). The phenolic compound standards were from Extrasynthese (Genay, France). 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was obtained from Alfa Aesar (Ward Hill, MA, USA). Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) was purchased from Sigma Chemical Co. (St. Louis, MO, USA). Foetal bovine serum (FBS), L-glutamine, hank's balanced salt solution (HBSS), trypsin-EDTA (ethylenediaminetetraAcetic acid), penicillin/streptomycin solution (100 U/mL and 100 mg/mL, respectively), RPMI-1640 and DMEM media were from Hyclone (Logan, Utah, USA). Acetic acid, ellipticine, sulphorhodamine B (SRB), trypan blue, trichloroAcetic acid (TCA) and Tris were from Sigma Chemical Co. (St Louis, MO USA). Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA).
Formic and Acetic acids were purchased from Prolabo (VWR International, France). The phenolic compound standards were from Extrasynthese (Genay, France). 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was obtained from Alfa Aesar (Ward Hill, MA, USA). Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) was purchased from Sigma Chemical Co. (St. Louis, MO, USA). Foetal bovine serum (FBS), L-glutamine, hank's balanced salt solution (HBSS), trypsin-EDTA (ethylenediaminetetraAcetic acid), penicillin/streptomycin solution (100 U/mL and 100 mg/mL, respectively), RPMI-1640 and DMEM media were from Hyclone (Logan, Utah, USA). Acetic acid, ellipticine, sulphorhodamine B (SRB), trypan blue, trichloroAcetic acid (TCA) and Tris were from Sigma Chemical Co. (St Louis, MO USA). Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA).
9
Comprehensive Reagents Sourcing for Research
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Acetonitrile 99.9% of HPLC grade was from Fisher Scientific (Lisbon, Portugal). The standards trolox (6-hydroxy-2,5,7,8tetramethylchroman-2-carboxylic acid), β-carotene and ellipticine were purchased from Sigma-Aldrich (St Louis, MO, USA), as also acetic acid, phosphate buffered saline (PBS), sulforhodamine B (SRB), and lipopolysaccharide (LPS). Phenolic compound standards were from Extrasynthèse (Genay, France). DPPH (2,2-diphenyl-1-picrylhydrazyl) was obtained from Alfa Aesar (Ward Hill, MA, USA). The Griess reagent system was purchased from Promega Corporation (Madison, WI, USA). The culture media Muller Hinton broth (MHB) and Tryptic Soy Broth (TSB) were obtained from Biomerieux (Marcy l'Etoile, France). The dye p-iodonitrotetrazolium chloride (INT) was purchased from Sigma-Aldrich (Spruce Street; St Louis, MO) and was used as a microbial growth indicator. All other chemi-cals were of analytical purity and obtained from common suppliers. Water was treated via the purification system Milli-Q water (TGI Pure Water Systems, Greenville, SC, USA).
10
Phenolic Compounds Analysis and Antimicrobial Assays
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Acetonitrile and methanol HPLC-grade and formic acids were purchased from Fisher Scientific (Lisbon, Portugal). Phenolic compound standards were obtained from Extrasynthese (Genay, France) and their purities were above 97%, as determined by HPLC-DAD analysis and stock solutions of these compounds (1 mg mL -1 ) were prepared in methanol/water (20 : 80, v/v). The culture media Mueller-Hinton broth (MHB) and tryptic soy broth (TSB) as well as blood agar with 7% sheep blood and MacConkey agar plates were obtained from Biomerieux (Marcyl'Etoile, France). The dye p-iodonitrotetrazolium chloride (INT) was purchased from Sigma-Aldrich (St Louis, MO, USA) and was used as a microbial growth indicator. Water was treated using the Milli-Q water purification system (TGI Pure WaterSystems, Greenville, SC, USA).
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