For all analysis involving more than two test conditions, One-way Analysis of Variance (ANOVA) was performed, followed by pairwise Tukey’s test. Statistical significance post ANOVA is denoted with red alphabets in all figures. In each comparison, conditions with different alphabets are statistically significant at alpha <0.05 whereas conditions with the same alphabet are statistically indistinguishable. Supplementary file 2 contains the exact p-values for all post hoc comparisons. For comparision between two samples, two-tailed unpaired Student’s t-test was used and the p-value is stated in the respective figure legends. All statistical tests were performed using Origin 8.0 software (Micro Cal). Area under the curve calculations were done using Microsoft Excel (Microsoft). All box plots were plotted using BoxPlotR59 (link).
Origin 8
Manufactured by Malvern Panalytical
Sourced in United States
Origin 8.0 is a versatile laboratory equipment designed for a range of analytical applications. It provides high-quality data acquisition and processing capabilities for users.
Automatically generated - may contain errors
Lab products found in correlation
Prism 6, by GraphPad (9 mentions)
Clampfit 10, by Molecular Devices (8 mentions)
Pclamp 10, by Molecular Devices (4 mentions)
Spss statistics 19, by IBM (3 mentions)
Sigmaplot 12, by Grafiti LLC (3 mentions)
Vp itc microcalorimeter, by Malvern Panalytical (3 mentions)
Prism 5, by GraphPad (3 mentions)
Mithras 940, by Berthold Technologies (3 mentions)
Model 1470, by PerkinElmer (2 mentions)
Spss 20, by IBM (2 mentions)
Lsm 510, by Zeiss (2 mentions)
Clampfit 9, by Molecular Devices (2 mentions)
Digidata 1440a, by Molecular Devices (2 mentions)
Pclamp software, by Molecular Devices (1 mentions)
Peaq itc, by Malvern Panalytical (1 mentions)
Biocytin, by Merck Group (1 mentions)
Application suite, by Leica (1 mentions)
Stata 7, by StataCorp (1 mentions)
Ls50b, by PerkinElmer (1 mentions)
Cfx96 system, by Bio-Rad (1 mentions)
166 protocols using origin 8
1
Statistical Analysis of Experimental Data
2
Electrophysiological Analysis of Synaptic Transmission
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MiniAnalysis software (Synaptosoft, Decatur, GA) was used to analyze spontaneous EPSCs. Clampfit 10.2 software (Molecular Devices) was used to analyze evoked EPSCs and current clamp recordings. The amplitude of evoked EPSCs was calculated as the difference between the average current during the 2 ms window at peak amplitude and the average baseline current taken 5 ms before the stimulus artifact. For each cell, recordings were normalized to a baseline of 10 min prior to drug application. PPR was calculated by dividing the amplitude of the second EPSC by the amplitude of the first (PPR = EPSC2/EPSC1). For each cell, the PPR was calculated during baseline and peak effect following drug application. Graphs were plotted, and statistics computed using Origin 8.0 software (Microcal Software, Northampton, MA, USA) and GraphPad Prism 6 software (GraphPad Software Inc., La Jolla, CA, USA). Results are expressed as mean +/− SEM. A two-way analysis of variance with multiple comparisons and bonferroni post hoc test were used to compare propensity to fire between KO and WT in tonic and delayed firing neurons. All other comparisons were made using a paired or unpaired student t test. P < 0.05 was considered significant.
3
Electrophysiological Data Acquisition and Analysis
4
Statistical Analysis of Research Data
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Origin 8.0 software (Microcal Software, Inc., Northampton, MA, USA) was used for data analysis. The results are presented as the mean ± standard error or the mean ± standard deviation. Paired or independent Student’s t-tests were used to test for significance where appropriate. P values < 0.05 were considered statistically significant.
5
Statistical Analysis of Experimental Data
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Superior Performance Software Systems software package (SPSS 17.0, Inc., Chicago, IL) was applied for the related statistical analyses. Correlation analysis was performed using Spearman's rank correlation analysis at the two-sided 0.05 level of significance (*p <0.05, **p<0.01). The variation and regression analysis are developed using Origin 8.0 software (Microcal Software, Northampton, MA) for the best fit curve from the experimental data.
6
Statistical Analysis of In Vitro and Animal Experiments
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The results were expressed as the mean ± SD for in vitro experiments and expressed as means ± (SE) for animal experiments. The data were analyzed using an Origin 8.0 software (MicroCal, USA). Data was assessed by one-way analysis of variance (ANOVA). The value of p < 0.05 or p < 0.01 was considered statistically significant. The significance of the main observations and interactions was further investigated using Tukey’s post hoc analyses.
7
Statistical Analysis of Experimental Data
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Origin 8.0 software (MicroCal, United States) was applied to perform the statistical analysis. N indicated the number of wells studied in each category. The data were expressed as the means ± SD. For statistical comparisons, the results were analyzed using one-way analysis of variance (ANOVA) and P < 0.05 was considered statistically significant.
8
Quantitative Analysis of Vesicular Release
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All flight data and quantifications of vesicular release are represented as box plots using BoxplotR (Spitzer et al., 2014 (link)), where horizontal lines represent medians, crosses indicate means, box limits indicate 25th and 75th percentiles, whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, individual data points are represented as open circles, and the numbers below represent the n number for each box. Traces from live-imaging experiments represent means (±standard error of means) from all cells or ROIs.
Comparisons between two samples were performed using the two-tailed Student’s t test. If more than two conditions were involved, one-way ANOVA followed by Tukey’s test was used. All statistical tests were performed using Origin 8.0 software (Micro Cal). Statistical tests and exact p-values used in each figure are listed inTable 1-1 .
Comparisons between two samples were performed using the two-tailed Student’s t test. If more than two conditions were involved, one-way ANOVA followed by Tukey’s test was used. All statistical tests were performed using Origin 8.0 software (Micro Cal). Statistical tests and exact p-values used in each figure are listed in
9
Statistical Analysis of Experimental Data
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SPSS Statistics 19 software (SPSS Inc., Chicago, IL, USA) was used to perform one-way analysis of variance (ANOVA) and Student-Newman-Keuls test (S-N-K) multiple range tests for all data. The results are expressed as the mean ± standard deviation, with P < 0.05 indicating a statistical difference. The graphics were drawn using Origin 8.0 software (MicroCal Software, Northampton, MA, USA).
10
Experimental Design and Statistical Analysis
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All experiments were performed using at least three replicate experiments. All data were reported as mean ± standard deviation. Data analyses were performed using Origin 8.0 software (Microcal, USA) and SPSS 26 (SPSS, Chicago, IL, U.S.A.). Statistical significance was determined using Duncan’s multiple range tests with P < 0.05 used as the threshold for statistical significance.
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