Horseradish peroxidase (HRP)-conjugated anti-FLAG tag mouse monoclonal (M2) antibody, and anti-FLAG tag antibody-conjugated agarose beads were obtained from Sigma-Aldrich (St. Louis, MO, USA). HRP-conjugated anti-T7 tag mouse monoclonal antibody and anti-T7 tag antibody-conjugated agarose beads were from Novagen™, Merck KGaA (Darmstadt, Germany). Anti-GFP rabbit polyclonal antibody and anti-β-actin mouse monoclonal antibody (C043) were also obtained from MBL (Nagoya, Japan) and Applied Biological Materials (Richmond, BC, Canada), respectively.
Hrp conjugated anti t7 tag mouse monoclonal antibody
Manufactured by Merck Group
Sourced in Germany, Japan
The HRP-conjugated anti-T7 tag mouse monoclonal antibody is a laboratory reagent that can be used to detect the presence of the T7 tag, a commonly used epitope tag, in protein samples. It is a mouse-derived antibody that has been conjugated with horseradish peroxidase (HRP), which allows for colorimetric or chemiluminescent detection of the tagged protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti flag tag antibody conjugated agarose beads, by Merck Group (1 mentions)
Anti t7 tag antibody conjugated agarose beads, by Merck Group (1 mentions)
Alexa fluor 488 594 conjugated secondary antibodies, by Thermo Fisher Scientific (1 mentions)
Stealth rnai sirna, by Thermo Fisher Scientific (1 mentions)
Horseradish peroxidase, by Merck Group (1 mentions)
3 protocols using hrp conjugated anti t7 tag mouse monoclonal antibody
1
Affinity Purification of Tagged Proteins
2
Antibody and siRNA Reagents for Rab11 and TBC1D12
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Anti-Rab11 mouse monoclonal antibody (#610657, BD Transduction Laboratories, Lexington, KY), anti-TfR mouse monoclonal antibody (#13–6800, ZYMED Laboratories Inc., San Francisco, CA), anti-β-actin mouse monoclonal antibody (#G043, Applied Biological Materials Inc., Richmond, BC, Canada), anti-green fluorescent protein (GFP) rabbit polyclonal antibody (#598, MBL, Nagoya, Japan), anti-TBC1D12 rabbit antibody (#HPA038277, Sigma-Aldrich, St. Louis, MO), horseradish peroxidase (HRP)-conjugated anti-FLAG tag (M2) mouse monoclonal antibody (#A8592, Sigma-Aldrich), HRP-conjugated anti-T7 tag mouse monoclonal antibody (#69048-3CN, Merck KGaA, Darmstadt, Germany), HRP-conjugated anti-GFP rabbit polyclonal antibody (#598–7, MBL), and Alexa Fluor 488/594-conjugated secondary antibodies (Thermo Fisher Scientific Corp., Hudson, NH) were obtained commercially. Anti-LC3β (simply referred to as LC3 throughout this paper) rabbit polyclonal antibody was affinity-purified as described previously [19 (link)]. Rab11A/B siRNAs were prepared as described previously [11 (link)]. Stealth RNAi™ siRNAs (simply referred to as siRNAs hereafter) against rat TBC1D12 (target site: 5’-AAUACUUCAACAUCAUGCUGUGAUC-3’ [siTBC1D12 #1] and 5’-AUUAAUUCCAGACUAGCCUCUCGGU [siTBC1D12 #2]) were purchased from Life Technologies.
3
Antibody Characterization for Cellular Localization
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The following antibodies used in this study were obtained commercially: anti-β-actin mouse monoclonal antibody (clone: G043) (ABM, Richmond, BC, Canada); anti-Arl8b rabbit polyclonal antibody (GeneTex, Irvine, CA); anti-GFP (green fluorescent protein) rabbit polyclonal antibody (MBL, Nagoya, Japan); anti-KHC (kinesin heavy chain) mouse monoclonal antibody (clone: H2) and HRP-conjugated anti-T7 tag mouse monoclonal antibody (Merck Millipore, Billerica, MA); anti-KLC2 goat polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA); anti-Lamp-1 rat monoclonal antibody (clone: 1D4B) (BD Biosciences, San Jose, CA); anti-SKIP rabbit polyclonal antibody (Abcam, Tokyo, Japan); anti-Rab1A rabbit polyclonal antibody (Abnova, Taipei, Taiwan); and HRP (horseradish peroxidase)-conjugated anti-FLAG tag mouse monoclonal antibody (clone: M2) (Sigma-Aldrich, St. Louis, MO).
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