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Mm glass coverslips

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Mm glass coverslips are flat, thin pieces of glass used to cover and protect samples in microscopy applications. They provide a transparent surface for observing and analyzing specimens under a microscope.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Zepto, by Diener Electronic (1 mentions) Poly d lysine, by Merck Group (1 mentions)

Close spelling variants of this product

13-mm glass coverslips 25 mm #1.5 glass coverslip 12 mm circular glass coverslips 22 mm glass coverslips 18 mm round glass coverslips Polylysine-coated 18 mm glass coverslips 12 mm glass coverslips Glass coverslips Coverslip

2 protocols using mm glass coverslips

1

Glass Coverslip Cleaning and Functionalization

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13 mm glass coverslips (VWR) were placed in a rack and rinsed in a stream of mQ. They were then sonicated in 2% SDS at 37 kHz and 100% power in a Fisherbrand™ bath sonicator (Fisher Scientific) for 10 minutes. Next, they were rinsed and soaked in mQ, followed by ethanol and dried with nitrogen. They were then plasma cleaned at 70% power for 2 minutes in a plasma cleaner (Zepto, Diener Electronic). The whole procedure was then repeated once more and coverslips functionalised as described below. Coverslips were used immediately after preparation and not stored.
Benn G., Pyne A.L., Ryadnov M.G, & Hoogenboom B.W. (2019). Imaging live bacteria at the nanoscale: comparison of immobilisation strategies. The Analyst, 144(23), 6944-6952.
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2

Culturing Embryonic Rat Cortical Neurons

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Neurons were prepared from E18 embryonic Sprague Dawley rat or E16.5 mouse cortices as described previously (Sellers et al., 2017). Briefly, cells were seeded on 16 mm glass coverslips (VWR) coated with poly-D-lysine (0.2 mg/ml, Sigma). Cells were cultured in neurobasal supplemented with B27 supplement, 0.2 mM glutamine and 1% penicillin/streptomycin (Thermo Fisher). Cultures were maintained at least 21 days in vitro (DIV) before use.
Elliott C., Rojo A.I., Ribe E., Broadstock M., Xia W., Morin P., Semenov M., Baillie G., Cuadrado A., Al-Shawi R., Ballard C.G., Simons P, & Killick R. (2018). A role for APP in Wnt signalling links synapse loss with β-amyloid production. Translational Psychiatry, 8, 179.
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