Chinese hamster ovary cells (provided by the Vanderbilt Antibody and Protein Resource, VAPR) were grown in DMEM containing 10% fetal bovine serum, 20 mM HEPES, 1mM pyruvate, 2mM L-glutamine, 20 μg/ml proline and antibiotic-antimicotic (Life Technologies). Cultures were maintained at 37°C, 5% CO2 and 95% relative humidity. The CHO cells were transfected with the expression plasmids and high expressing colonies were identified and selected on the ClonePix FL by the VAPR (Life Technologies) using a fluorophore labeled mouse anti-EPO (MAB2871, R&D systems, Minneapolis, MN).
Clonepix fl
Manufactured by Thermo Fisher Scientific
The ClonePix FL is a fluorescence-based cell imaging and selection instrument designed for high-throughput screening and identification of clonally-derived cells. It provides automated imaging and analysis of single cells and cell colonies in multi-well culture plates.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using clonepix fl
1
EPO Production in CHO Cells
2
EPO Production in CHO Cells
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Chinese hamster ovary cells (provided by the Vanderbilt Antibody and Protein Resource, VAPR) were grown in DMEM containing 10% fetal bovine serum, 20 mM HEPES, 1mM pyruvate, 2mM L-glutamine, 20 μg/ml proline and antibiotic-antimicotic (Life Technologies). Cultures were maintained at 37°C, 5% CO2 and 95% relative humidity. The CHO cells were transfected with the expression plasmids and high expressing colonies were identified and selected on the ClonePix FL by the VAPR (Life Technologies) using a fluorophore labeled mouse anti-EPO (MAB2871, R&D systems, Minneapolis, MN).
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