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Anti bax d2e11

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Anti-Bax (D2E11) is a primary antibody that targets the Bax protein. Bax is a pro-apoptotic member of the Bcl-2 family and plays a crucial role in the intrinsic apoptosis pathway. The Anti-Bax (D2E11) antibody can be used in various applications, such as Western blotting, immunohistochemistry, and flow cytometry, to detect and study the Bax protein.

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Lab products found in correlation

Anti bcl xl 54h6, by Cell Signaling Technology (1 mentions) Anti mcl 1 d2w9e, by Cell Signaling Technology (1 mentions) Anti cleaved caspase 3 d175, by Cell Signaling Technology (1 mentions) Anti cd204 clone sra e5, by Cosmo Bio (1 mentions) Anti cleaved parp asp214 d64e10, by Cell Signaling Technology (1 mentions) β actin sc 47778, by Santa Cruz Biotechnology (1 mentions) Fixation buffer, by BioLegend (1 mentions) Permeabilization wash buffer, by BioLegend (1 mentions)

3 protocols using anti bax d2e11

1

Apoptosis Protein Profiling in MDA-MB-231 Cells

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A 40 μg quantity of the total protein, obtained from untreated MDA-MB-231 cells and cells treated with Chalcotanina for 24 and 48 h, was processed as described in 4.5.2, using the flowing antibodies: anti-Bim-EL (GT1234), anti-Bcl-2 (N1N2) (GeneTex, Irvine, CA, USA), anti-Bax (D2E11), anti Bcl-XL (54H6), and anti-Mcl-1 (D2W9E) (Cell signaling Technology, Danvers, MA, USA) (to identify pro- and anti-apoptotic proteins levels).
Mendez-Callejas G., Piñeros-Avila M., Yosa-Reyes J., Pestana-Nobles R., Torrenegra R., Camargo-Ubate M.F., Bello-Castro A.E, & Celis C.A. (2023). A Novel Tri-Hydroxy-Methylated Chalcone Isolated from Chromolaena tacotana with Anti-Cancer Potential Targeting Pro-Survival Proteins. International Journal of Molecular Sciences, 24(20), 15185.
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2

Protein Extraction and Western Blot Analysis

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The cellular proteins were solubilized in a Tris buffer containing 2% sodium dodecyl sulfate and 10% glycerol. The following antibodies were used for Western blotting: anti-SPP1 (AF1433; R&D Systems), anti-CD204 (clone SRA-E5; CosmoBio), anti-Bcl-2 (D17C4; Cell Signaling Technology, Danvers, MA, USA), anti-Bax (D2E11; Cell Signaling Technology), anti-cleaved caspase-3 (D175; Cell Signaling Technology), anti-cleaved PARP (Asp214; D64E10; Cell Signaling Technology), anti-Bmi1 (EPR3745(2); abcam, Cambridge, UK), and β-actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA). The detected bands were quantitatively measured using Image J [28 (link)]. The original WB can be found in the Supplementary Material File S1.
Matsubara E., Komohara Y., Esumi S., Shinchi Y., Ishizuka S., Mito R., Pan C., Yano H., Kobayashi D., Fujiwara Y., Ikeda K., Sakagami T, & Suzuki M. (2022). SPP1 Derived from Macrophages Is Associated with a Worse Clinical Course and Chemo-Resistance in Lung Adenocarcinoma. Cancers, 14(18), 4374.
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3

Intracellular Staining of CD8+ T Cells

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Following cell surface staining for CD11a and PD-1, CD8+ T cells were incubated with purified anti-mouse CD16/32 antibody for blocking nonspecific binding of immunoglobulin to Fc receptors. To detect intracellular Bim, granzyme B, or T-bet, cells were incubated with Fixation Buffer (BioLegend), followed by permeabilization using permeabilization wash buffer (Biolegend). Anti-Bim rabbit monoclonal antibody (clone C34C5; catalog 2933), anti-Bax (D2E11, 5023), anti-Bad (D24A9, 9239), anti–Bcl-2 (50E3, 2870), anti–Bcl-xL (54H6, 2764), and appropriate isotype control (rabbit [DA1E] monoclonal Ab IgG XP) were purchased from Cell Signaling and were added into cells and incubated for an hour. After staining, cells were washed 3 times with washing buffer before analysis. FITC- or PE-conjugated secondary antibody to rabbit IgG was used for flow cytometry assay. At least 100,000 viable cells were live gated on a FACSCailbur of FACSCanto instrument (BD Biosciences). Flow cytometry analysis was performed using FlowJo software (Tree Star). Bim expression was presented by frequency of Bim+ cells among tumor-reactive CD8+ T cells or Bim levels (MFI) in tumor-reactive CD8+ T cells.
Dronca R.S., Liu X., Harrington S.M., Chen L., Cao S., Kottschade L.A., McWilliams R.R., Block M.S., Nevala W.K., Thompson M.A., Mansfield A.S., Park S.S., Markovic S.N, & Dong H. (2016). T cell Bim levels reflect responses to anti–PD-1 cancer therapy. JCI Insight, 1(6), e86014.
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