Anti cd19 bv711

Manufactured by BioLegend

Sourced in Canada

Anti-CD19-BV711 is a fluorochrome-conjugated antibody that binds to the CD19 antigen on the surface of B cells. It can be used for the identification and enumeration of B cells in flow cytometry applications.

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Anti-CD19 (91 citations) CD19-BV711 (2 citations)

4 protocols using anti cd19 bv711

Multiparametric Flow Cytometry Analysis

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The following anti-human fluorochrome-conjugated antibodies were used: Alexa Fluor 700-conjugated antibody to human CD3 (UCHT1); anti-IL-7Rα-BV421 (A019D5), anti-CD4-PE-Cy5 (OKT4), anti-CD45RA-APC-Cy7 (HI100), anti-Fas-BV650 (DX2), anti-CCR7-BV785 (G043H7), anti-CD31-Alexa Fluor 488 (WM59), anti-TCR γ/δ-Percp-Cy5.5 (B1), anti-CD25-BV650 (BD96; BioLegend), anti-CD19-BV711 (SJ25C1), anti-CD14-BV711 (MφP9), anti-CD3-BV510 (HIT3a), anti-CD8-Alexa Fluor 700 (RPA-T8; all from BD Biosciences); anti-CD19-PE-eFluor610 (HIB19); anti-CD14-PE-eFluor610 (61D3), and anti-CD56-APC (CMSSB; Thermo Fisher). Simultaneously, dead cells were stained using a Live/Dead Fixable Red Dead Cell Stain Kit (Thermo Fisher). The cells were fixed and permeabilized using a Foxp3 Staining Buffer Set (Thermo Fisher), and then intracellular molecules were stained (4°C, 20 minutes) using anti-Bcl-2-Alexa Fluor 488 (100; BioLegend), anti-Ki-67-PE-Cy7 (20Raj1), and anti-Foxp3-PE (236A/E7; Thermo Fisher). Detailed information about the multicolor flow cytometry panel used in this study is shown in supplemental Table 4.

Kim S., Lee S.W., Koh J.Y., Choi D., Heo M., Chung J.Y., Lee B.H., Yang S.H., Sung Y.C., Lee H., Shin E.C, & Park S.H. (2022). A single administration of hIL-7-hyFc induces long-lasting T-cell expansion with maintained effector functions. Blood Advances, 6(23), 6093-6107.

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Comprehensive Immune Cell Profiling

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Peripheral blood mononuclear cells (PBMCs), peritoneal cells (PerC),and splenocytes were collected from mice and were stained for 1 h with anti-IgG-FITC (Biolegend-406001, San Diego, CA), anti-CD95-PerCPefluor710 (Ebioscience-46–0951-82), anti-CD21-Pacific Blue (Biolegend-123414,San Diego, CA), anti-CD14-DyLight405LS (Novus Biologicals, Centennial, CO), anti-CD11b-BV570 (Biolegend-101233, San Diego, CA), anti-CD4-Qdot605 (Invitrogen-Q10092,), anti-CD23-BV786 (BD Horizon-563988, San Jose, CA), anti-CD19-BV711 (Biolegend-115555, San Diego, CA), anti-CD23-BV650 (BD-Horizon-563545, San Jose, CA), anti-GL7-AlexaFluor647 (Biolegend-144606, San Diego, CA), anti-CD45R-AlexaFluor700 (Biolegend-103232), anti-IgDAPC-Cy7 (Biolegend-405716), anti-CD43-PE (Biolegend-143206, San Diego, CA), anti-IgM-PE-Dazzle594 (Biolegend-406530, San Diego, CA), anti-CD5-PE-Cy5 (Biolegend-100610, San Diego, CA), and anti-CD1d-PE-Vio77 (Miltenyi Biotec-130–105-157, San Diego, CA). Cells were washed twice and stained with live/dead fixable yellow (Invitrogen) for 20 m. One-to-five million total events per sample were recorded on an LSRII instrument (BD Biosciences) and analysis was performed using FlowJo software (Treestar, Inc, Ashland, OR).

Sarkar S., Piepenbrink M.S., Basu M., Thakar J., Keefer M.C., Hessell A.J., Haigwood N.L, & Kobie J.J. (2019). IL-33 enhances the kinetics and quality of the antibody response to a DNA and protein-based HIV-1 Env vaccine. Vaccine, 37(17), 2322-2330.

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Comprehensive Flow Cytometry Analysis

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Flow cytometry was performed using standard cell-surface staining techniques using directly conjugated fluorochrome antibodies and analyzed on the Cytek Aurora (Cytek Biosciences). All analysis was performed using FlowJo version 10.8.0 (BD Biosciences). Prior to surface staining, tissue single-cell suspensions were incubated in Fixable Viability Dye eFluor 780 (eBioscience) to exclude dead cells, washed, and then incubated in anti-mouse CD16/CD32 Fc block (Biolegend) and unlabeled MR1-6-formylpterin-tetramer (6-FP) (NIH Tetramer core) to reduce non-specific binding. Cells were then incubated for 20 minutes at room temperature with mouse PE conjugated 5-OP-RU MR1-tetramer (NIH Tetramer core) and the following antibodies: anti-CD4-FITC/APC-Fire810 (clone GK1.5, Tonbo Biosciences/Biolegend), anti-CD8α-AF700 (clone 53-6.7, Bioleg-end), anti-TCRβ-BUV496/BV421 (clone H57-597, BD Biosciences/Biolegend), anti-CD3-BUV395 (clone 17A2, Biolegend), anti-CD69-BV510 (clone H1.2F3 Biolegend), anti-B220-PE-Cy5 (clone RA3-6B2, Biolegend), anti-CD19-BV711 (clone 6D5, Biolegend), anti-CD44-BV650 (clone IM7, Biolegend), anti-CD38-PE-Cy7 (clone 90, Biolegend), anti-IgD-BV510 (clone 11-26c.2a, Bioleg-end), anti-CD27-FitC (clone LG.3A10, Biolegend), anti-CD138-BV605 (clone 281-2, Biolegend).

Jensen O., Trivedi S., Li K., Aubé J., Hale J.S., Ryan E.T, & Leung D.T. (2022). Use of a MAIT-Activating Ligand, 5-OP-RU, as a Mucosal Adjuvant in a Murine Model of Vibrio cholerae O1 Vaccination. Pathogens and Immunity, 7(1), 122-144.

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Profiling Antigen-Specific B-cell Memory

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Immunophenotyping of circulating B-cell subpopulations was performed on frozen PBMCs stained with the following appropriately titrated mAbs: 7-aminoactinomycin D (7AAD) (BioLegend, San Diego, CA), anti-CD19-BV711 (BioLegend), anti-D27-FITC (BioLegend), and anti-IgD-Pacific Blue (BioLegend). Antigen-specific cells were detected using anti-His-Alexa647 (MBL, Aichi, Japan) and/or anti-E-tag-phycoerythrin (PE Conjugation kit) (Abcam, Cambridge, United Kingdom) with 3 mg DSG1/3 E-His protein or influenza HA-His antigen protein (A/Brisbane/02/2018 [H1N1]-pdm09-like, eENZYME, Gaithersburg, MD). At least 2.5 × 10 5 PBMCs were acquired on the FACS-Aria III flow cytometer and then analyzed using FlowJo software. Statistical analyses were performed in GraphPad Prism, version 8. DSG-specific MBCs (7AAD-CD19+CD27+IgD-E-tag+His-tag+), non-DSG-specific MBCs (7AAD-CD19+CD27+IgD-E-tag-His-tag-), HA-specific MBCs (7AAD-CD19+CD27+IgD-His-tag+), and non-HA-specific MBCs (7AAD-CD19+CD27+IgD-His-tag-) were single-cell sorted into 96-well PCR plates (Eppendorf, Tokyo, Japan) containing SMART-Seq HT lysis Components (Takara Bio, Shiga, Japan) at a quarter volume using FACS-Aria III; they were frozen immediately on dry ice and stored at -80 °C.

Egami S., Watanabe T., Fukushima-Nomura A., Nomura H., Takahashi H., Yamagami J., Ohara O, & Amagai M. (2023). Desmoglein-Specific B-Cell-Targeted Single-Cell Analysis Revealing Unique Gene Regulation in Patients with Pemphigus. The Journal of investigative dermatology, 143(10).

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