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Bm hmscs

Manufactured by Lonza
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BM-hMSCs is a type of human mesenchymal stem cell derived from bone marrow. It is a primary cell culture used for research purposes.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Dmem f12, by Thermo Fisher Scientific (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Lenti x 293t cell line, by Takara Bio (1 mentions) Dulbecco s modified eagle medium dmem, by Fujifilm (1 mentions) αmem glutamax, by Thermo Fisher Scientific (1 mentions) β mercaptoethanol, by Thermo Fisher Scientific (1 mentions) Knockout serum replacement (ksr), by Thermo Fisher Scientific (1 mentions) Matrigel, by BD (1 mentions) Mtesr medium, by STEMCELL (1 mentions) Hek293 cell, by Thermo Fisher Scientific (1 mentions) Hucct 1, by Nacalai Tesque (1 mentions) Bm hmscs, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Nacalai Tesque (1 mentions) Hek293 cell, by American Type Culture Collection (1 mentions) Rpmi 1640 medium, by Nacalai Tesque (1 mentions)

6 protocols using bm hmscs

1

Cultivation of Bone Marrow-Derived Mesenchymal Stem Cells

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Cell lines and cell culture Bone marrow-derived human mesenchymal stem cells (BM-hMSCs) were procured from LONZA (Catalog #0,000,494,678) and cultured in Minimum Essential Medium Alpha (MEMα; Fuji-Film Wako, Japan) enriched with 20% fetal bovine serum (FBS; Hyclone, Victoria, Australia). The LentiX293T cell line was acquired from Takara Bio (Kusatsu, Shiga, Japan) and maintained in Dulbecco's Modified Eagle Medium (DMEM; Fuji-Film Wako, Japan) supplemented with 10% FBS, penicillin (100 U/mL), and streptomycin (100 mg/ mL) (Thermo Fisher Scientific, Waltham, MA). All cell lines were incubated in a humidified chamber with 5% CO2 at 37°C.
Osawa T., Yamada D., Takao T., Ming L, & Takarada T. (2024). PRRX1 upregulates PD-L1 in human mesenchymal stem cells. In vitro cellular & developmental biology. Animal.
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2

Culturing Human Embryonic Stem Cells and Derived Cell Lines

Cited 1 time
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Human H9 ESCs as well as derived YAP−/− and TAZ−/− hESCs were maintained on feeder layers of mitomycin C–inactivated MEFs in hESC medium [68 (link)] (DMEM/F12 [Thermo Fisher Scientific, Waltham, MA], 20% Knockout Serum Replacement [Thermo Fisher Scientific], 0.1 mM nonessential amino acids [NEAAs; Thermo Fisher Scientific], 2 mM GlutaMAX [Thermo Fisher Scientific], 1% penicillin/streptomycin [Thermo Fisher Scientific], 55 μM β-mercaptoethanol [Thermo Fisher Scientific], and 10 ng/ml bFGF [Joint Protein Central, Incheon, Korea]) or on Matrigel (BD Biosciences, San Jose, CA, USA) in mTeSR medium (STEMCELL Technologies, Vancouver, Canada). hESCs derived hMSCs and BM-hMSCs (purchased from Lonza, Basel, Switzerland) were cultured in hMSC medium (αMEM + GlutaMAX [Thermo Fisher Scientific], 10% fetal bovine serum [Gibco, Cat: 10099–141, Lot: 1616964], 1% penicillin/streptomycin [Thermo Fisher Scientific], and 1 ng/ml bFGF [Joint Protein Central]). No mycoplasma contamination was observed during cell culture.
Fu L., Hu Y., Song M., Liu Z., Zhang W., Yu F.X., Wu J., Wang S., Izpisua Belmonte J.C., Chan P., Qu J., Tang F, & Liu G.H. (2019). Up-regulation of FOXD1 by YAP alleviates senescence and osteoarthritis. PLoS Biology, 17(4), e3000201.
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3

Cell Culture Conditions of Common Cell Lines

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HEK293 cells were purchased from the American Type Culture Collection (Manassas, VA, USA). HuCCT1 and HEL cells were purchased from the Japanese Cancer Research Resources Bank (Japan). BM-hMSCs and hPBMCs were purchased from Lonza Group AG (Switzerland). HEK293 cells were maintained in Dulbecco’s modified Eagle medium (DMEM) high-glucose GlutaMAX supplement (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific) and 1% penicillin-streptomycin (Nacalai Tesque). HuCCT1 and HEL cells were maintained in RPMI 1640 medium (Nacalai Tesque) supplemented with 10% FBS and 1% penicillin-streptomycin. BM-hMSCs were maintained in DMEM (Thermo Fisher Scientific) supplemented with 10% FBS and penicillin-streptomycin.
Ito K., Matsuda Y., Mine A., Shikida N., Takahashi K., Miyairi K., Shimbo K., Kikuchi Y, & Konishi A. (2022). Single-chain tandem macrocyclic peptides as a scaffold for growth factor and cytokine mimetics. Communications Biology, 5, 56.
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4

Mesenchymal Stem Cell Culture and Scaffold Preparation

Cited 2 times
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Bone marrow derived human mesenchymal stem cells (BM-hMSCs) (24yr old B female, Lonza, Switzerland) were used at passage 4 – 6 and cultured with complete hMSC media containing low glucose Dulbecco’s Modified Eagle Medium and glutamine (School of Chemical Sciences Cell Media Facility, University of Illinois), Fetal Bovine Serum (Gemini Bio Products, California, USA), and antibiotic-antimycotic solution (Thermo Fisher Scientific, Massachusetts, USA). Cell contamination was tested with a MycoAlert™ Mycoplasma Detection Kit (Lonza) and cells tested negative for mycoplasma.
Scaffolds and reinforced composites were sterilized via ethylene oxide treatment with an AN74i Anprolene gas sterilizer (Andersen Sterilizers Inc., North Carolina, USA) for in vitro testing. Prior to adding cells to scaffolds and reinforced composites, these followed a standard hydration procedure for mineralized collagen scaffolds previously reported [33 (link), 45 (link)]. Briefly, samples were hydrated in 70% ethanol, washed in PBS, crosslinked with 1-Ethyl-3- (3-dimethylaminopropyl) carbodiimide and N-Hydroxysuccinimide, followed by washing in PBS, and finally soaking in hMSC complete media for 2 days.
Dewey M.J., Nosatov A.V., Subedi K., Shah R., Jakus A, & Harley B.A. (2020). Inclusion of a 3D-printed Hyperelastic Bone mesh improves mechanical and osteogenic performance of a mineralized collagen scaffold. Acta biomaterialia, 121, 224-236.
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5

Harvesting Nasal Septal Cartilage and Culturing Bone Marrow-Derived MSCs

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Human cartilage tissue
is commonly removed and discarded in routine ENT surgery such as septoplasty.
During this procedure, human nasal septal cartilage (PC: male, age
43) was harvested with consent provided and ethics approved (HREC
2018-023). Human bone marrow-derived MSCs (BM-hMSCs) were purchased
from Lonza at passage 2 (P2, male, age 25) and plated as per manufacturer’s
instructions. P2 cells were subcultured to passage 4 (P4) and stored
in liquid nitrogen in freezing solution [90% fetal bovine serum (FBS,
Bovogen) and 10% dimethyl sulfoxide (DMSO, Sigma)] at densities of
5.0 × 105 cells/mL.
Posniak S., Chung J.H., Liu X., Mukherjee P., Gambhir S., Khansari A, & Wallace G.G. (2022). Bioprinting of Chondrocyte Stem Cell Co-Cultures for Auricular Cartilage Regeneration. ACS Omega, 7(7), 5908-5920.
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6

Cultivation of Bone Marrow Mesenchymal Stem Cells

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Bone marrow derived human mesenchymal stem cells (BM-hMSCs; 5 th to 10 th passage, Lonza, Basel, Switzerland) served as control and were cultivated in cell culture medium RPMI1640 (GIBCO, Invitrogen) containing 10% (v/v) fetal calf serum (FCS; GIBCO, Invitrogen) and Lglutamine (0.3 g L -1 ; GIBCO, Invitrogen) using 75 cm 2 culture flasks (BD Falcon). Cells were grown at 37 °C in a humidified 5% CO 2 atmosphere and sub-cultivated every 7 to 14 d depending on cell proliferation. After washing with PBS, growing hMSC were detached from the culture flasks by addition of 0.2 mL cm -2 0.25% trypsin/0.05% EDTA for 5 min at 37 °C. Subsequently, cells were harvested, washed twice with RPMI/FCS and seeded at a density of 1.5 x 10 4 cells per well in 24-well cell culture plates (BD Falcon).
Sengstock C., Rövekamp M., Volkenstein S., Minovi A., Neubaur A., Dazert S., Aach M., Schildhauer T.A., Köller M., & Sengstock C. (2020). Olfactory Stem Cells for the Treatment of Spinal Cord Injury: Isolation, Purification and Behaviour in a Plasma Clot Matrix. International Journal of Regenerative Medicine.
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