Hpa034881

Manufactured by Merck Group

HPA034881 is a laboratory equipment product from Merck Group. It is designed for use in scientific research and analysis. The core function of this product is to provide a reliable and precise measurement tool for researchers and scientists. Further details on the intended use or specific applications of this product are not available.

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Lab products found in correlation

Prb 155p, by Fortrea (2 mentions) Ab24647, by Abcam (2 mentions) Af3625, by R&D Systems (1 mentions) Ab15580, by Abcam (1 mentions) Mab424, by Merck Group (1 mentions) Sc 8431, by Santa Cruz Biotechnology (1 mentions) Phospho histone h3, by Cell Signaling Technology (1 mentions) A11055, by Thermo Fisher Scientific (1 mentions) Alexa fluor 647, by Thermo Fisher Scientific (1 mentions) A 21244, by Thermo Fisher Scientific (1 mentions) A31573, by Thermo Fisher Scientific (1 mentions) A1 inverted confocal microscope, by Nikon (1 mentions)

2 protocols using hpa034881

Immunostaining Protocol for Epithelial Markers

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Mouse monoclonal antibody against IL-33 (clone Nessy-1) (#ALX-804-840-C100) was purchased from Enzo. Rabbit polyclonal antibodies against KRT5 (#ab24647) and Ki-67 (#ab15580) were purchased from Abcam (Abcam, Cambridge, MA). Rabbit polyclonal antibody against KRT14 (#PRB-155P) was purchased from Covance (Covance, Princeton, NJ). Rabbit polyclonal antibody against KRT4 (#HPA034881) was purchased from Sigma (Sigma-Aldrich Corp, St. Louis, MO). Rabbit monoclonal antibodies against E-cadherin (#3195), p75 (#8238), and phospho-histone H3 (#3377) were purchased from Cell Signaling (Cell Signaling Technology, MA). Mouse monoclonal antibody against p63 (#sc-8431) was purchased from Santa Cruz (Santa Cruz Biotechnology, TX). Mouse monoclonal antibody against PCNA (#MAB424) was purchased from Millipore (Billerica, MA). Goat polyclonal antibody against IL-33 (#AF3625) was purchased from R&D (R&D Systems, Minneapolis, MN). Donkey anti-goat Alexa Fluor 488 (A11055), anti-rabbit Alexa Fluor 568 (A10042), and anti-mouse Alexa Fluor 647 (A31571) secondary antibodies were purchased from Life Technologies (Carlsbad, CA). Mouse anti-HSP90 (TA500494) and mouse anti-GAPDH (TA310153) primary antibodies were purchased from Origene (Rockville, MD).

Travers J., Rochman M., Caldwell J.M., Besse J.A., Miracle C.E, & Rothenberg M.E. (2017). IL-33 is induced in undifferentiated, non-dividing esophageal epithelial cells in eosinophilic esophagitis. Scientific Reports, 7, 17563.

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Immunofluorescence Profiling of Esophageal Biopsies

Cited 1 time

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Immunofluorescence staining was performed as previously described ^{4 (link)} on at least four distal esophageal biopsies from control individuals, distal esophageal biopsies with active inflammation from patients with active EoE, or proximal esophageal biopsies without active inflammation from the same patients with active EoE. The average ages of EoE patients and control individuals were 13 and 11 years, respectively. Patient groups were also controlled for race and sex. The following primary antibodies were used at a final concentration of 1 μg/mL: anti–keratin 14 (PRB-155P, Covance), anti–keratin 5 (ab24647, Abcam), anti–keratin 4 (HPA034881, Sigma), or anti-cornulin (AF3607, R&D). Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Slides were blocked with phosphate-buffered saline with 10% goat or donkey serum. Secondary antibodies (1:500 dilution) used were donkey anti-rabbit Alexa Fluor 647 (A31573, Life Technologies), goat anti-rabbit Alexa Fluor 647 (A21244, Life Technologies), or donkey anti-goat Alexa Fluor 488 (A11055, Life Technologies). Imaging was performed with a Nikon A1 inverted confocal microscope.

Rochman M., Travers J., Miracle C.E., Bedard M.C., Wen T., Azouz N.P., Caldwell J.M., KC K., Sherrill J.D., Davis B.P., Rymer J.K., Kaufman K.M., Aronow B.J, & Rothenberg M.E. (2017). Profound Loss of Esophageal Tissue Differentiation in Eosinophilic Esophagitis. The Journal of allergy and clinical immunology, 140(3), 738-749.e3.

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