Female athymic nude mice aged 6 weeks, weighing 19–23 g, were purchased from Nara Biotech (Seoul, Korea). All mice were housed under specific pathogen-free environmental conditions at the laboratory animal facility of the Anonymous College of Veterinary Medicine. All experimental procedures involving animals were approved by the Institutional Animal Care and Use Committee at Anonymous (approval no.: Anonymous-210825-2). To induce CMGC in mice, 5 × 106 CIPp cells were suspended in 100 µL of PBS, mixed with 100 µL of Matrigel (Corning Inc., New York, USA), and injected into the mammary fat pad. Tumour growth and body weight were measured every 3–4 days until post-injection day 21. Tumour volume was estimated using the following formula: (width2×length)/2 [44 (link), 45 (link)]. Mice were sacrificed on day 21 with CO2 asphyxiation, and xenograft tumours were harvested. For safety evaluation, organ abnormalities were visually inspected at necropsy, and the organs of digestive system (stomach, small and large intestines) were assessed by group.
Female athymic nude mice
Manufactured by Nara Biotech
Female athymic nude mice are a laboratory animal model. They are characterized by the absence of a functional thymus gland, resulting in a lack of T cells and a compromised immune system.
Automatically generated - may contain errors
Lab products found in correlation
Matrigel, by Corning (1 mentions)
Male sprague dawley rats, by Nara Biotech (1 mentions)
Dulbecco s modified eagle s medium dmem, by Corning (1 mentions)
Streptomycin, by Corning (1 mentions)
Penicillin, by Corning (1 mentions)
Rpmi 1640, by Corning (1 mentions)
Ht1080, by Korean Cell Line Bank (1 mentions)
Egm 2, by PromoCell (1 mentions)
3 protocols using female athymic nude mice
1
Xenograft Model of Carcinoma-Mediated Granuloma in Mice
2
Preclinical Animal Models for Pharmacokinetics and Cancer
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Sprague-Dawley male rats (270 ± 20 g, 6–8 weeks old) for pharmacokinetic and rectum morphology study, and female athymic nude mice (20 ± 1 g, 5 weeks old) for anti-tumor and histopathology test were purchased from Nara Biotech. Co. (Seoul, South Korea). These animals were placed with free access to drinking water under the temperature of 21–22 °C and relative moisture of 55 ± 2% for acclimatization. The procedures for the animal studies were consistent with NIH Policy and the Animal Welfare Act under the approval of the Institutional Animal Care and Use Committee (IACUC) at Hanyang University.
3
Cultivation and Maintenance of Human and Murine Cell Lines
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Cells and animals. Human fibrosarcoma HT1080 (KCLB no. 10121) and murine melanoma B16F10 (KCLB no. 80008) cells were purchased from the Korean Cell Line Bank (Seoul, Korea) and maintained in RPMI-1640 or Dulbecco's modified eagle's medium (DMeM; Cellgro, Manassas, VA, uSA) supplemented with 10% (v/v) fetal bovine serum (FBS; Cellgro), 100 u/ml penicillin and 100 µg/ml streptomycin (Cellgro) at 37˚C in a humidified incubator with 5% CO 2 . Human umbilical vein endothelial cells (HuVeCs) were purchased from InnoPharmaScreen (Asan, Korea) at passage 2, maintained in endothelial cell growth medium-2 (egM-2; PromoCell, Heidelberg, germany), and used for experiments at passages 3-8. For the animal experiments, female athymic nude mice were purchased from nara Biotech (Seoul, Korea). Female C57BL/6 mice and male Sprague Dawley rats were obtained from Taconic Farms Inc. (Samtako Bio Korea, Osan, Korea). All animals were housed under controlled conditions using a 12/12-h light/dark cycle at 22±1˚C and 55±5% humidity under specific pathogen-free conditions. All animal experiments were approved by the Animal Care and use Committee of the Korea Institute of Oriental Medicine (KIOM, Daejeon, Korea), with the reference numbers #13-42, #13-48 and #14-27, and were performed in accordance with the guidelines of the Animal Care and use Committee at KIOM.
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