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Ultrapure equimolar deoxynucleotide triphosphate dntp sodium salt solution

Manufactured by New England Biolabs

Ultrapure equimolar deoxynucleotide triphosphate (dNTP) sodium salt solution is a high-quality laboratory reagent that provides a balanced mixture of the four DNA building blocks: dATP, dCTP, dGTP, and dTTP. This solution is designed to meet the requirements of various molecular biology applications, including PCR, sequencing, and other DNA-based techniques.

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2 protocols using ultrapure equimolar deoxynucleotide triphosphate dntp sodium salt solution

1

Fluorescent DNA Oligonucleotide Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
ssDNA oligonucleotides and AlexaFluor 647-labeled ssDNA oligonucleotides were purchased from Integrated DNA Technologies Inc. Full oligonucleotide sequences can be found in the accompanying Supporting Information (SI) file. Ultrapure equimolar deoxynucleotide triphosphate (dNTP) sodium salt solution was purchased from New England Biolabs Inc. (Catalog #N0447S) as an equimolar mixture of 10 mM dATP, dCTP, dGTP and dTTP giving a final dNTP concentration of 40 mM. AlexaFluor 647 C2 maleimide (Free AF) was purchased from ThermoFisher Scientific (Catalog #A20347). All other chemicals including 97% 1, 1′-diethyl-2, 2′-cyanine iodide (PIC, CAS #:977-96-8); molecular biology-grade water; sodium chloride (NaCl); magnesium chloride (MgCl2); and TRIS (2-Amino-2-(hydroxymethyl)propane-1, 3-diol; pH 7.0) were sourced from Sigma-Aldrich.
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2

Fluorescent DNA Oligonucleotide Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
ssDNA oligonucleotides and AlexaFluor 647-labeled ssDNA oligonucleotides were purchased from Integrated DNA Technologies Inc. Full oligonucleotide sequences can be found in the accompanying Supporting Information (SI) file. Ultrapure equimolar deoxynucleotide triphosphate (dNTP) sodium salt solution was purchased from New England Biolabs Inc. (Catalog #N0447S) as an equimolar mixture of 10 mM dATP, dCTP, dGTP and dTTP giving a final dNTP concentration of 40 mM. AlexaFluor 647 C2 maleimide (Free AF) was purchased from ThermoFisher Scientific (Catalog #A20347). All other chemicals including 97% 1, 1′-diethyl-2, 2′-cyanine iodide (PIC, CAS #:977-96-8); molecular biology-grade water; sodium chloride (NaCl); magnesium chloride (MgCl2); and TRIS (2-Amino-2-(hydroxymethyl)propane-1, 3-diol; pH 7.0) were sourced from Sigma-Aldrich.
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