Varian cary eclipse fluorescence spectrometer
The Varian Cary Eclipse Fluorescence Spectrometer is a laboratory instrument designed for the analysis of fluorescent samples. It is capable of measuring the fluorescence emission and excitation spectra of various materials.
Lab products found in correlation
4 protocols using varian cary eclipse fluorescence spectrometer
Fluorescence Spectrometry of Protein
Critical Micelle Concentration Determination
Fluorescence Spectroscopy of Tetracaine
Excitation and emission slits were fixed at 5 nm. In all measurements, the excitation wavelength was set at 310 nm. The samples were scanned from 320 to 450 nm at a wavelength scan rate of 120 nm/min with a PMT detector gain of 600 V. The data were smoothed with a Savitzky Golay function filter size 25 using the Cary Eclipse software.
The experiments were performed at a temperature of 32 °C. The system was chemically stable over 6 days and the effect of ion pairing with the ions dissolved in the aqueous solution was not significant (Data not shown).
Characterization of Photoluminescent Quantum Dots
QDs characterization was carried out by simultaneous detection and quantification of the elements constituting the QD core (S, Zn and Mn) using an ICP-MS/MS system (Agilent 8800 ICPQQQ, Tokyo, Japan). For separation and characterization of nanoparticles and their bioconjugates the ICP-MS/MS was coupled on-line to the AF4 system (AF2000, Postnova Analytics, Landsberg, Germany). Separation conditions are summarized in Table S1 (Electronic Supplementary Material, ESM). Dynamic light scattering spectra were measured by using a NanoZS90 instrument from Malvern Instruments, Houston, TX, USA.
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