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The YN-20 is a centrifuge designed for general laboratory applications. It features a fixed-angle rotor and can accommodate various sample tube sizes. The YN-20 provides reliable performance for standard centrifugation procedures.

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Irdye antibodies, by LI COR (2 mentions) Odyssey scanner, by LI COR (2 mentions) Phospho p38 mapk thr180 tyr182 antibody, by Cell Signaling Technology (1 mentions)

4 protocols using yn 20

1

Immunoprecipitation of SNF2 Protein

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Sporulation was induced as described earlier and 50 ml of cells at various time points were pelleted. Protein was isolated from cells pellets with FA-1 lysis buffer (50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Sodium Deoxycholate, 1 mM PMSF and protease inhibitors) using bead-beating methods. Immunoprecipitation was performed with anti-SNF2 antibody (yN-20, Santa Cruz, 10 μl per IP) and γ-G sepharose beads and washed with the lysis buffer. Western blots were performed as described above.
Venkataramanan S., Douglass S., Galivanche A.R, & Johnson T.L. (2017). The chromatin remodeling complex Swi/Snf regulates splicing of meiotic transcripts in Saccharomyces cerevisiae. Nucleic Acids Research, 45(13), 7708-7721.
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2

Protein Extraction and Western Blot Analysis

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Protein extraction and western blot analysis was carried out as previously described [6 (link)] using a phospho-p38 MAPK (Thr180/Tyr182) antibody to detect the SakA phosphorylated form, a phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody to detect the MpkA phosphorylated form (Cell Signaling Technology), and a γ-tubulin antibody as a reference (yN-20) (Santa Cruz biotechnology, Inc.). Every experiment was repeated at least three times, using independent biological replicates. Additional information is reported in S1 Text.
Altwasser R., Baldin C., Weber J., Guthke R., Kniemeyer O., Brakhage A.A., Linde J, & Valiante V. (2015). Network Modeling Reveals Cross Talk of MAP Kinases during Adaptation to Caspofungin Stress in Aspergillus fumigatus. PLoS ONE, 10(9), e0136932.
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3

Beauvericin Modulates Sch9 and Ypk1 Phosphorylation

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The yor1Δ strain was grown overnight in YEPD at 30°C, diluted to an OD600 of 0.1, and 5 ml of culture was treated with appropriate vehicle (methanol) or drug (5 μg/ml beauvericin) at appropriate time points. All samples were collected after 4 hours. Cell growth was stopped by adding 100 % w/v TCA to a final concentration of 6 % v/v. After harvesting, cell pellets were processed for western blotting as previously described56 (link). A Licor Odyssey Scanner was used to detect phosphorylated Sch9 (P-Sch9T758), phosphorylated Ypk1 (P-Ypk1T662), total Sch9, and total Ypk1. Monoclonal antibodies against P-Sch9, P-Ypk1, and total Sch9 were used, and a commercially available antibody from Santa Cruz (yN-20) was used for total Ypk1 detection. Secondary IRDye antibodies from Li-Cor Biosciences were used for primary antibody detection.
Shekhar-Guturja T., Gunaherath G.M., Kithsiri Wijeratne E.M., Lambert J.P., Averette A.F., Lee S.C., Kim T., Bahn Y.S., Tripodi F., Ammar R., Döhl K., Niewola-Staszkowska K., Schmitt L., Loewith R.J., Roth F.P., Sanglard D., Andes D., Nislow C., Coccetti P., Gingras A.C., Heitman J., Leslie Gunatilaka A.A, & Cowen L.E. (2016). Dual Action Antifungal Small Molecule Modulates Multidrug Efflux and TOR Signaling. Nature chemical biology, 12(10), 867-875.
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4

Beauvericin Modulates Sch9 and Ypk1 Phosphorylation

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The yor1Δ strain was grown overnight in YEPD at 30°C, diluted to an OD600 of 0.1, and 5 ml of culture was treated with appropriate vehicle (methanol) or drug (5 μg/ml beauvericin) at appropriate time points. All samples were collected after 4 hours. Cell growth was stopped by adding 100 % w/v TCA to a final concentration of 6 % v/v. After harvesting, cell pellets were processed for western blotting as previously described56 (link). A Licor Odyssey Scanner was used to detect phosphorylated Sch9 (P-Sch9T758), phosphorylated Ypk1 (P-Ypk1T662), total Sch9, and total Ypk1. Monoclonal antibodies against P-Sch9, P-Ypk1, and total Sch9 were used, and a commercially available antibody from Santa Cruz (yN-20) was used for total Ypk1 detection. Secondary IRDye antibodies from Li-Cor Biosciences were used for primary antibody detection.
Shekhar-Guturja T., Gunaherath G.M., Kithsiri Wijeratne E.M., Lambert J.P., Averette A.F., Lee S.C., Kim T., Bahn Y.S., Tripodi F., Ammar R., Döhl K., Niewola-Staszkowska K., Schmitt L., Loewith R.J., Roth F.P., Sanglard D., Andes D., Nislow C., Coccetti P., Gingras A.C., Heitman J., Leslie Gunatilaka A.A, & Cowen L.E. (2016). Dual Action Antifungal Small Molecule Modulates Multidrug Efflux and TOR Signaling. Nature chemical biology, 12(10), 867-875.
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