Hydrogen tetrachloroaurate 3 hydrate haucl4.3h2o

Nilavembu Choornam powder (NC), marketed by a commercial manufacturer, was procured from the Siddha storekeeper at Villupuram, Tamil Nadu, India. The hydrogen tetrachloroaurate (III) hydrate (HAuCl₄.3H₂O; Sigma Aldrich, St. Louis, MO, USA), syringe filtrate membrane (Sartorius Stedim Biotech, Göttingen, Germany), and other reagents were procured. For transmission electron microscopy (TEM) analysis, carbon-coated copper grids (TABB Bioscience (UK), Luria Bertani (LB), Nutrient, Brain Heart Infusion (BHI), and MacConkey broth/agar culture media (Scharlab S.L., Barcelona, Spain) were also procured.

Five indium tin oxide coated polyethylene terephthalate film (ITO-PET) electrodes (thickness 0.175 mm, surface resistivity of 50 Ω cm⁻²) were purchased from NanoGostar Sepahan (Isfahan, Iran). Hyaluronic acid kit containing Biotin-HA-Ab and 320 ng mL⁻¹ main concentration of HA-antigen was acquired from ZellBio GmbH (Germany) by Padgin Teb Company. Hydrogen tetrachloroaurate (iii) hydrate (HAuCl₄–3H₂O), cetyltrimethylammonium bromide (CTAB), and sulfuric acid, 98%, were obtained from ''Sigma-Aldrich (Ontario, Canada)'', for performing electrochemical deposition of Au nanoparticles on ITO-PET electrodes. An electrochemical investigation of the detection zone of the ITO-PETs was implemented using a ferricyanide/ferrocyanide solution including 0.5 M of K₄Fe CN₆/K₃Fe CN₆ along with KCl. Fresh frozen plasma samples were obtained from the Iranian Blood Transfusion Research Center (Tabriz, Iran). KCC-1-NH-CS₂ was used for encapsulation and activation of biotinylated antibody that was synthesized by our research group, previously.^{27 (link)}