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Oregon green 488 conjugated dextran

Manufactured by Thermo Fisher Scientific
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Oregon Green 488-conjugated dextran is a fluorescent dye-labeled polysaccharide compound. It is designed for use as a tool in biological research applications.

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Lab products found in correlation

Eclipse ti microscope, by Nikon (1 mentions)

Close spelling variants of this product

Oregon Green 488‐dextran Oregon Green Dextran Oregon Green 488 Oregon Green Dextran-488 Dextrans

3 protocols using oregon green 488 conjugated dextran

1

Quantifying Macropinosome Formation

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To assess macropinosome formation, dextran internalization into cells was measured. Cells were prechilled at 14°C and then incubated with Oregon Green 488–conjugated dextran (molecular weight, 70000 kDa; Thermo Fisher), to a final concentration of 125 μg/mL. The samples were incubated at 37°C for 1 hour, fixed in 10% buffered formalin, and stained with HCS CellMask blue stain to define cell boundaries. Cells were photographed using Nikon Ti-Eclipse microscope. The number of dextran-positive vesicles was counted for 20 cells per treatment, using Imaris software (Bitplane, Zurich, Switzerland).
Shtanko O., Reyes A.N., Jackson W.T, & Davey R.A. (2018). Autophagy-Associated Proteins Control Ebola Virus Internalization Into Host Cells. The Journal of Infectious Diseases, 218(Suppl 5), S346-S354.
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2

Spinal Column Analysis of HSV-2 Immunity

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Spinal column was harvested from intranasal TK− HSV-2 immunized mice 45 min after tail vein injection with 200 µl of 5 mg/ml Oregon Green 488-conjugated dextran (70kDa, D7173, Thermo Fisher Scientific, MA) in PBS. Spine was then fixed with 4% paraformaldehyde in PBS overnight, and cut frozen sections (8 µm in thickness) for immunohistochemical analysis30 (link).
Iijima N, & Iwasaki A. (2016). Access of protective antiviral antibody to neuronal tissues requires CD4 T cell help. Nature, 533(7604), 552-556.
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3

Spinal Column Analysis of HSV-2 Immunity

Cited 1 time
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Spinal column was harvested from intranasal TK− HSV-2 immunized mice 45 min after tail vein injection with 200 µl of 5 mg/ml Oregon Green 488-conjugated dextran (70kDa, D7173, Thermo Fisher Scientific, MA) in PBS. Spine was then fixed with 4% paraformaldehyde in PBS overnight, and cut frozen sections (8 µm in thickness) for immunohistochemical analysis30 (link).
Iijima N, & Iwasaki A. (2016). Access of protective antiviral antibody to neuronal tissues requires CD4 T cell help. Nature, 533(7604), 552-556.
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