Log In Sign up
The largest database of trusted experimental protocols

Rna cartridge kit

Manufactured by Bioptic
Visit Supplier
Sourced in United States

The RNA Cartridge kit is a laboratory equipment product designed for the extraction and purification of RNA from biological samples. It provides a standardized and efficient method for isolating RNA, which is a critical molecule for various biotechnological and research applications.

Automatically generated - may contain errors

Lab products found in correlation

Qseq100 bio fragment analyzer, by Bioptic (3 mentions) Rneasy plus universal kit, by Qiagen (3 mentions) Take3, by Agilent Technologies (2 mentions) Qubit rna hs assay kit, by Thermo Fisher Scientific (2 mentions) Nextseq 550ar, by Illumina (1 mentions) Vahts mrna seq v3 library prep kit, by Vazyme (1 mentions)

4 protocols using rna cartridge kit

1

Tumor RNA Isolation and Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA of tumor samples was isolated using RNeasy Plus Universal Kits (Qiagen, GER). RNA concentration was quantified using QubitTM RNA HS Assay Kit (ThermoFisher Scientific, USA). RNA purity and integrity were analyzed using Take3 (BioTek, USA) and the RNA Cartridge kit of the Qseq100 Bio-Fragment Analyzer (Bioptic, CHN), respectively. Then, RNA-seq libraries were constructed using VAHTS mRNA-seq V3 Library Prep Kit for Illumina (Vazyme, CHN). Libraries were sequenced on the NextSeq 550AR platform with 150 bp paired-end reads. Quality control of WES data was described in Supplementary Data 3.
Li S., Yu W., Xie F., Luo H., Liu Z., Lv W., Shi D., Yu D., Gao P., Chen C., Wei M., Zhou W., Wang J., Zhao Z., Dai X., Xu Q., Zhang X., Huang M., Huang K., Wang J., Li J., Sheng L, & Liu L. (2023). Neoadjuvant therapy with immune checkpoint blockade, antiangiogenesis, and chemotherapy for locally advanced gastric cancer. Nature Communications, 14, 8.
+ Open protocol
+ Expand
2

Exosomal MALAT1 RNA Quality Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols
A macrophage-derived exosomal MALAT1 RNA quality assessment was performed using a BiOptic Qsep100 using RNA cartridge kit (BiOptic Inc., Taipei, Taiwan), following the manufacturer’s protocol. An electropherogram and a virtual gel image were generated to visualize the samples and improve interpretation. All samples were analyzed in duplicate.
Shyu K.G., Wang B.W., Fang W.J., Pan C.M, & Lin C.M. (2022). Exosomal MALAT1 Derived from High Glucose-Treated Macrophages Up-Regulates Resistin Expression via miR-150-5p Downregulation. International Journal of Molecular Sciences, 23(3), 1095.
+ Open protocol
+ Expand
3

RNA Extraction and RNA-seq Library Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA of tumor samples was isolated from the tumor tissue with RNeasy Plus Universal Kits (Qiagen, GER) according to the manufacturer's instructions. A Qubit™ RNA HS Assay Kit (Thermo Fisher Scientific, USA) was used to quantify the concentrations of the extracted RNA. The purity and integrity of the RNA were checked with Take 3 (BioTek, USA) and the RNA Cartridge kit of the Qseq100 Bio-Fragment Analyzer (Bioptic, CHN), respectively. The VAHTS mRNA-seq V3 Library Prep Kit for Illumina (Vazyme, CHN) was used to construct RNA-seq libraries. Finally, the libraries were sequenced on the NextSeq 550AR with 150 bp paired-end reads.
Zhang G., Yuan J., Pan C., Xu Q., Cui X., Zhang J., Liu M., Song Z., Wu L., Wu D., Luo H., Hu Y., Jiao S, & Yang B. (2023). Multi-omics analysis uncovers tumor ecosystem dynamics during neoadjuvant toripalimab plus nab-paclitaxel and S-1 for esophageal squamous cell carcinoma: a single-center, open-label, single-arm phase 2 trial. eBioMedicine, 90, 104515.
+ Open protocol
+ Expand
4

RNA-seq of Tumor Tissue Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
We isolated the total RNA of tumor samples from the tumor tissues via RNeasy Plus Universal Kit (Qiagen, USA). Utilizing the QubitTM RNA HS Assay Kit, the concentrations of the extracted RNA were then quantified. The integrity and purity of the extracted RNA were tested by the RNA Cartridge kit of the Qseq100 Bio-Fragment Analyzer (Bioptic, China) and the Take3 kits (BioTek, USA), respectively. We then generated the RNA-seq libraries by the VAHTS mRNA-seq V3 Library Prep Kit (Vazyme, China). Eventually, the libraries were sequenced on the NextSeq 550AR (Illumina, USA) with 150-bp paired-end reads.
Peng H., Wu X., Cui X., Liu S., Liang Y., Cai X., Shi M., Zhong R., Li C., Liu J., Wu D., Gao Z., Lu X., Luo H., He J, & Liang W. (2024). Molecular and immune characterization of Chinese early-stage non-squamous non-small cell lung cancer: a multi-omics cohort study. Translational Lung Cancer Research, 13(4), 763-784.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!