DMEM, Trypsin-EDTA, Penicillin-streptomycin, TRIzol, FBS, were from Invitrogen (Paisley, United Kingdom). All tissue culture ware was from Nunc (Roskilde, Denmark). Biotin Blocking System, peroxidase substrate (DAB), peroxidase buffer, and hematoxylin were from DAKO (Glostrup, Denmark). Aquatex was from Merck (Darmstadt, Germany). The ABC kit was from Vecstain (Burlingame, CA). Proteinase inhibitors were from Roche (Madrid, Spain). ECL western blotting substrate was from Pierce (Thermo Fisher Scientific, Rockford, IL). Neomycin and recombinant Angiogenin was from Sigma-Aldrich, and unless otherwise stated, all other reagents were also from Sigma-Aldrich (St. Louis, MO).
Peroxidase buffer
Manufactured by Agilent Technologies
Sourced in Denmark
Peroxidase buffer is a reagent used in various laboratory applications. It is designed to maintain the optimal pH and ionic conditions required for the activity of the enzyme peroxidase. Peroxidase is a widely used enzyme in various analytical and diagnostic techniques, such as enzyme-linked immunosorbent assays (ELISA) and enzymatic colorimetric assays. The peroxidase buffer ensures the proper functioning of the peroxidase enzyme during these procedures.
Automatically generated - may contain errors
Lab products found in correlation
Proteinase inhibitor, by Roche (3 mentions)
Dab peroxidase substrate, by Agilent Technologies (2 mentions)
Aquatex, by Merck Group (2 mentions)
Biotin blocking system, by Agilent Technologies (2 mentions)
Trypsin edta, by Merck Group (2 mentions)
Venetoclax abt 199, by Selleck Chemicals (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions)
Penicillin streptomycin and dimethyl sulfoxide, by Merck Group (2 mentions)
Dcf d6883, by Merck Group (2 mentions)
Jc 1 t 3168, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Hematoxylin, by Agilent Technologies (1 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (1 mentions)
A 1331852, by Selleck Chemicals (1 mentions)
Abt 263 navitoclax, by Selleck Chemicals (1 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
3 protocols using peroxidase buffer
1
Assay Reagents for Cell Culture
2
Molecular Assay Protocol for Cell Viability
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Dulbecco’s modified eagle’s medium (DMEM), trypsin-EDTA, penicillin-streptomycin and dimethyl sulfoxide (DMSO), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) (M2128), Hoechst 33258 (B1155), Crystal Violet (C0755), and DCF (D6883) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All tissue culture-ware was from Nunc (Roskilde, Denmark). Biotin Blocking System, peroxidase substrate (DAB), and peroxidase buffer were from DAKO (Glostrup, Denmark). Proteinase inhibitors were from Roche (Madrid, Spain). ECL Western blotting substrate was from Pierce (Thermo Fisher Scientific, Rockford, IL, USA). BCL-2 siRNA (h) (sc-29214), BCL-xL siRNA (h) (sc-43630), and scrambled controls were purchased from Santa Cruz Biotechnology (Dallas, TX, USA), while a second siRNA for BCL-2 (ID#s1915) and for BCL-xL (ID#s1920) were obtained from Ambion Life technologies (Carlsbad, CA, USA). Lipofectamine2000 (11668-027), Novex Sharp Pre-stained Protein Standard (LC5800), and JC-1 (T-3168) were from Invitrogen Life Technologies (Carlsbad, CA, USA). Sorafenib (BAY 43-9006, Nexavar) and Regorafenib (BAY 73-4506, Stivarga) are manufactured by Bayer. A-1331852 and ABT-199 (Venetoclax) were purchased from Selleckchem (Houston, TX, USA).
3
Apoptosis Regulating Compounds Protocol
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Dulbecco's Modified Eagle's Medium (DMEM), trypsin-EDTA, penicillin-streptomycin and dimethyl sulfoxide (DMSO), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) (M2128), Hoechst 33258 (B1155), Crystal Violet (C0755), and DCF (D6883) were purchased from Sigma-Aldrich (St. Louis, MO). All tissue culture-ware was from Nunc (Roskilde, Denmark). Biotin Blocking System, peroxidase substrate (DAB) and peroxidase buffer were from DAKO (Glostrup, Denmark). Aquatex was from Merck (Darmstadt, Germany). The ABC kit was from Vecstain (Burlingame, CA). Proteinase inhibitors were from Roche (Madrid, Spain). ECL western blotting substrate was from Pierce (Thermo Fisher Scientific, Rockford, IL). BCL-2 siRNA (h) (sc-29214), BCL-xL siRNA (h) (sc-43630), MCL-1 siRNA (h) (sc-35877) and scrambled controls were purchased from Santa Cruz Biotechnology (Dallas, TX). Lipofectamine2000 (11668-027), Novex Sharp Pre-stained Protein Standard (LC5800) and JC-1 (T-3168) were from Invitrogen Life Technologies (Carlsbad, CA). Sorafenib (BAY 43-9006, Nexavar) and Regorafenib (BAY 73-4506, Stivarga) are manufactured by Bayer. A-1155463, ABT-737, AT101, HA-14, ABT-263 (Navitoclax) and ABT-199 (Venetoclax) were purchased from Selleckchem (Houston, TX).
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