Axio examiner z1 microscope
The Zeiss Axio Examiner Z1 is a high-performance upright microscope designed for advanced research applications. It features a stable, modular design that supports a range of accessories and imaging techniques, including brightfield, darkfield, and fluorescence microscopy. The microscope's key specifications include a high-resolution, high-contrast optical system, a motorized focusing mechanism, and compatibility with a variety of sample types and preparation methods.
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15 protocols using axio examiner z1 microscope
Immunofluorescence Analysis of Muscle Cryosections
Muscle Histochemical Staining Protocol
For nicotinamide adenine dinucleotide (NADH) dehydrogenase staining, sections were incubated for 30 min at 37 °C in a solution containing NBT (Sigma-Aldrich Chemie GmbH), 50 mM Tris/HCl, and NADH (Sigma-Aldrich Chemie GmbH), then washed in distilled water.
For succinate dehydrogenase (SDH) staining sections were incubated for 45 min at 37 °C in a solution containing Tris 0.2 M pH 7.2, cobalt (II)-chloride, MTT (methylthiazolyldiphenyl-tetrazolium bromide), and NADH. Afterwards, sections were incubated for 30 min in 4% PFA and washed in H2O.
Stained cryosections were analyzed and documented using a Zeiss Axio Examiner Z1 microscope equipped with an AxioCam MRm camera and ZEISS AxioVision Release 4.8 (Carl Zeiss MicroImaging, Göttingen, Germany). Densitometric quantifications were done using ImageJ [20 (link)].
Confocal Imaging Protocols and Techniques
Confocal Imaging Using Zeiss Axio Examiner
Diaphragm-Phrenic Nerve Preparation and Tubocurarine Effects
For recordings in the presence of tubocurarine, the chamber was filled with 2 mL (300 nM, 500 nM, 800 nM, or 1000 nM) of
Phrenic Nerve-Diaphragm Muscle Preparation
Vimentin Surface Visualization Assay
Visualizing Surface and Intracellular Vimentin
Multiphoton Imaging of Spheroid Invasion
Second Harmonic Generation Imaging of H&E Sections
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