Whole mounted mammary glands were imaged using the Zeiss Axio Imager dissection microscope. Using Zen Pro software, the whole mounts were analyzed to quantify number of branching points, number of ductal trees, and total ductal area. We also counted the number of bulb-shaped structures located at the end of ducts, consistent with female mammary structures known as terminal end buds (TEBs) and quantified the total area of all TEBs.
Axioimager dissection microscope
Manufactured by Zeiss
Sourced in Germany
The AxioImager dissection microscope is an optical microscope designed for detailed observation and analysis of specimens. It features high-quality optics and advanced illumination systems to provide clear, high-contrast images. The microscope is suitable for a variety of applications, including biological research, material science, and industrial inspection.
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3 protocols using axioimager dissection microscope
1
Quantifying Mammary Gland Branching and Structures
2
Quantification of Mammary Gland Ductal Density
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After fixation, whole-mounted mammary glands were processed through an alcohol series, defatted with toluene, stained with carmine alum, dehydrated in an alcohol and xylene series, and preserved in k-pax heat-sealed bags (Thermo Fisher Scientific) with methyl salicylate (Acros Organics, Morris Plains, NJ) [51 (link)]. Two digital images of whole-mount mammary glands (one from the left; one from the right third pectoral glands) were obtained using an AxioImager dissection microscope (Carl Zeiss Microscopy, Jena, Germany) at ×30 magnification and a Zeiss high-resolution color camera. To evaluate mammary gland morphology, ZEN software (Carl Zeiss Microscopy) was used to measure ductal density [51 (link)]. A 13 × 16 grid (180 crosshairs, 0.3 mm apart) was placed on each image, and the fraction of crosshairs that fell on ducts was quantified and averaged for the two images for each sample.
3
Mammary Gland Morphometric Analysis
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