On the ninth day after administration, the mice were sacrificed and tumor tissues of mice were harvested for hematoxylin and eosin (H&E) staining and terminal transferase dUTP nick-end labeling (TUNEL) assay kit staining to measure the induced apoptosis. The tissue sections were stained by using Glut1 (Bioss, Beijing, China) and CAIX(Bioss, Beijing, China) antibody to display the hypoxia area. For detection of tumor desmoplasia, the α-SMA antibody (Abcam, UK), and the Picro-Sirius Red Stain Kit (Solarbio, Beijing, China) were used. Besides, immunofluorescence sections of LLC tumor tissue were stained using CD31 (Abcam, UK) antibody to show the vascular density. Positive signals were captured using a fluorescence microscope equipped with a camera (Nikon DS-Qi1MC, Nikon Corporation).
Picro sirius red stain kit
Manufactured by Solarbio
Sourced in China
The Picro Sirius Red Stain Kit is a laboratory reagent used for the detection and visualization of collagen fibers in biological samples. The kit contains a Picro Sirius Red stain solution and the necessary reagents for the staining process.
Automatically generated - may contain errors
4 protocols using picro sirius red stain kit
1
Evaluating Tumor Hypoxia and Angiogenesis
2
Histological Analysis of Liver Tissues
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Liver tissues were dehydrated and fixed in 4% paraformaldehyde, embedded in paraffin, cut into 5 μm thick sections, deparaffinized, and stained with HE (Solarbio, G1120), Masson’s trichrome (Solarbio, G1340) and Picro Sirius Red Stain Kit (Solarbio, G1470). The histology was then determined using a light microscope.
3
Comprehensive Antibody Analysis for Tissue Evaluation
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Antibodies against collagen I (cat. no. ab34710; Abcam), III (cat. no. ab7778; Abcam), and IV (cat. no. ab6586; Abcam); Ki67 (cat. no. ab15580; Abcam); cluster of differentiation (CD) 31 (cat. no. ab182981; Abcam); transforming growth factor (TGF)-β1 (cat. no. ab92486; Abcam) and TGF-β3 (cat. no. ab15537; Abcam); α-smooth muscle actin (α-SMA) (cat. no. ab32575; Abcam); vascular endothelial growth factor (VEGF) (cat. no. ab46154; Abcam); glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (cat. no. ab181602; Abcam); and secondary antibodies [goat anti-rabbit IgG H&L (horseradish peroxidase) antibody (cat. no. ab97051; Abcam) and goat anti-mouse IgG H&L (horseradish peroxidase) antibody (cat. no. ab6789; Abcam)] were obtained from Abcam (Cambridge, MA, USA). The Picro Sirius Red Stain Kit was from Solarbio (Beijing, China). All other reagents were from Sigma-Aldrich (St. Louis, MO, USA). GAPDH was used as a protein loading control.
4
Chondrogenic Differentiation of Stem Cells
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To form a cell pellet, 2 × 105 cells/ml centrifuged in 15 ml polypropylene conical tube at 1100 rpm for 6 min. Pellets were incubated in the StemPro chondrogenesis differentiation medium at 37 °C, 5% CO2 for 21 days. The culture medium was changed every 3 days. Primary cell pellets were fixed in 4% PFA (m/v) for 24 h and embedded in paraffin. Cell pellet sections were stained with the Alcian Blue Stain Kit (Cat No. G2541, Solarbio, Beijing, China) and Picro Sirius Red Stain Kit (Cat No. G1470, Solarbio). For Alcian Blue staining, specimens were treated with 3% acetic acid (pH 2.5) for 5 min and stained with 1% Alcian Blue for 1 h before counter-staining with nuclear fast red for 5 min. For Picro Sirius Red staining, the sections were incubated in 0.1% of Sirius Red in saturated aqueous picric acid for 1 h and rinsed with hydrochloric acid (0.01 M) for 2 min.
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