Primary normal human astrocytes

Manufactured by ScienCell

Sourced in United States

Primary normal human astrocytes (NHA) are a type of glial cell isolated from the human central nervous system. They provide support and protection for neurons. NHA can be used for various research applications.

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Lab products found in correlation

Hek 293t cell, by Cell Resource Center (1 mentions) U87 and u251 glioma cell lines, by Cell Resource Center (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Astrocyte medium, by ScienCell (1 mentions) Fetal bovine serum (fbs), by Gemini Bio (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) Anti β actin antibody, by Merck Group (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions) Snail, by Cell Signaling Technology (1 mentions) Mmp 9, by Cell Signaling Technology (1 mentions) N cadherin, by Cell Signaling Technology (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin g, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Primary human astrocytes (43 citations) Human astrocytes (43 citations) Normal human astrocytes (24 citations)

6 protocols using primary normal human astrocytes

Brain Glioma Surgical Specimen Collection

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The 113 cases of clinical specimens were collected from tumorectomy of brain glioma in the Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan. The adjacent brain tissue was defined as 1cm away from the lesions. The criteria for the inclusion/exclusion of patient are: (1) age 0-70; (2) only received surgery, without preoperative chemotherapy or radiation therapy; (3) without any other type of cancer, autoimmune disease, infectious diseases, etc. All specimens were obtained under sterile conditions during surgery, snap frozen in liquid nitrogen, and stored at 80°C.
Primary normal human astrocytes (NHA) were purchased from the Sciencell Research Laboratories (Carlsbad, CA) and cultured under the conditions as instructed by the manufacturer. U87MG and U251 cells were obtained from Shanghai Institutes for Biological Sciences Cell Resource Center (Shanghai, China), and cultured in DMEM medium with high glucose and sodium pyruvate, supplied with 12% fetal bovine serum, 100 units/mL penicillin and 100μg/mL streptomycin.

Gao T., Gu G., Tian J., Zhang R., Zheng X., Wang Y., Pang Q, & Liu Q. (2017). LncRNA HSP90AA1-IT1 promotes gliomas by targeting miR-885-5p-CDK2 pathway. Oncotarget, 8(43), 75284-75297.

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Cell lines and primary astrocytes

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Human U87 and U251 glioma cell lines and human embryonic kidney (HEK) 293T cells were purchased from the Shanghai Institutes for Biological Sciences Cell Resource Center. Primary normal human astrocytes (NHA) were purchased from the Sciencell Research Laboratories (Carlsbad, CA, USA). For details, see Additional file 3.

Zheng J., Liu X., Xue Y., Gong W., Ma J., Xi Z., Que Z, & Liu Y. (2017). TTBK2 circular RNA promotes glioma malignancy by regulating miR-217/HNF1β/Derlin-1 pathway. Journal of Hematology & Oncology, 10, 52.

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Culturing Brain Glioma and Astrocyte Cells

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Human brain glioma U251 and U87 cell lines and HEK 293T cells were obtained from the China Academy of Chinese Medical Sciences. The cells were cultured in DMEM medium supplemented with 10% fetal bovine serum (Life Technologies Corporation, Carlsbad, CA), and culture condition was 5% CO₂ at 37°. Primary normal human astrocytes (NHA) were purchased from the Sciencell Research Laboratories (Carlsbad, CA) and cultured according to the manufacturer’s instructions.

Shang C., Hong Y., Guo Y, & Xue Y.X. (2016). Mir-338-3p Inhibits Malignant Biological Behaviors of Glioma Cells by Targeting MACC1 Gene. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 22, 710-716.

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Culturing Human Astrocytes and Glioma Cells

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Primary normal human astrocytes (NHA) and astrocyte medium were acquired from Sciencell Research Laboratories (Carlsbad, CA, USA). Human brain glioma cell lines (U251 and U87) were purchased from Shanghai Institutes for Biological Sciences Cell Resource Center and cultured in DMEM medium containing with 10% fetal bovine serum (EXcell, Shanghai, China). All cells were cultured in a 95% air and 5% CO₂ incubator at 37°C.

Hu X., Hong Y, & Shang C. (2019). Knockdown of long non-coding RNA SNHG5 inhibits malignant cellular phenotypes of glioma via Wnt/CTNNB1 signaling pathway. Journal of Cancer, 10(5), 1333-1340.

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Culturing Human Astrocytes and Glioma Cell Lines

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Primary normal human astrocytes (NHA) were purchased from Sciencell Research Laboratories (Carlsbad, CA, USA) and cultured under the conditions as instructed by the manufacturer. Cell lines T98G, A172, U87, and U251 were obtained from the KeyGEN Company (Shanghai, China) and cultured in DMEM supplemented with 10% fetal bovine serum (FBS) (Gemini Bio-Products, Woodland, CA, USA) and 1% penicillin/streptomycin solution (Thermo Fisher Scientific, Waltham, MA, USA). Cells were incubated at 37°C in a humidified incubator with 5% CO₂. Antibodies against β-catenin, E-cadherin, N-cadherin, Slug, Snail, and MMP9 were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-β-actin antibody and EGF were purchased from Sigma-Aldrich Co. (St Louis, MO, USA).

Wang X., Wang S., Li X., Jin S., Xiong F, & Wang X. (2017). The critical role of EGF-β-catenin signaling in the epithelial-mesenchymal transition in human glioblastoma. OncoTargets and therapy, 10, 2781-2789.

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Primary Human Astrocytes and Glioma Cell Culture

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Primary normal human astrocytes (NHA) were purchased from Sciencell Research Laboratories (Carlsbad, CA, USA). The U251, U87, SNB19 and LN229 glioma cell lines were obtained from the Institute of Biochemistry and Cell Biology (Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai, China). The cells were maintained in RPMI-l640 medium containing 10% FBS, 50 units/ml penicillin G, and 250 μg/ml streptomycin (all purchased from Invitrogen Life Technologies, Carlsbad, CA, USA) in a humidified atmosphere containing 5% CO₂, at 37°C. Transfections with miR-218 were performed in serum-free medium 24 h subsequent to plating, using Lipofectamine 2000 (Invitrogen Life Technologies). After 6 h, the cells were placed in complete medium and maintained at 37°C in a 5% CO₂ atmosphere.

GU J.J., GAO G.Z, & ZHANG S.M. (2015). miR-218 inhibits the migration and invasion of glioma U87 cells through the Slit2-Robo1 pathway. Oncology Letters, 9(4), 1561-1566.

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