Horseradish peroxidase hrp labeled anti mouse and anti rabbit secondary antibodies

Metformin was obtained from Sigma-Aldrich (St. Louis, MO, USA). Horseradish peroxidase (HRP)-labeled anti-mouse and anti-rabbit secondary antibodies were from Santa Cruz (Dallas, TX, USA). Phospho-anti-Akt, PTEN and phospho-anti-AMPK antibodies were purchased form Cell signaling (Beverly, CA, USA). Anti-Ecadherin, VEGF, PCNA, t-Akt, t-AMPK and β-actin were bought form Abcam (Cambridge, TX, USA).

Sorafenib was kindly provided by Bayer Pharmaceuticals. YC-1 was obtained from Sigma-Aldrich (Saint Quentin Fallavier, France). Anti-PARP, anti-caspase 8, anti-β-actin, and anti-caspase 9 were obtained from Beyotime (Jiangsu, China). Horseradish peroxidase (HRP)-labeled anti-mouse and anti-rabbit secondary antibodies were from Santa Cruz (Dallas, TX, USA). All other antibodies were purchased from Abcam (Cambridge, TX, USA).

The following antibodies were used: the primary antibodies for cleaved PARP-1 (cleaved p25), DR5, and Smac/DIABLO were purchased from GeneTex whereas AIF was purchased from Thermo Scientific. Antibodies against antibody against polyclonal caspase-3, monoclonal DR4, monoclonal p21, polyclonal p27, monoclonal Cdk2, polyclonal Cdk4, polyclonal cyclin D1, polyclonal cyclin E, polyclonal cIAP-1, polyclonal c-FLIPL, monoclonal survivin, polyclonal Bak, polyclonal Bax, monoclonal Bcl-xL, polyclonal Bid, polyclonal GADD153, monoclonal p-ERK, polyclonal ERK2 and polyclonal Akt1 were purchased from Santa Cruz. Antibodies against monoclonal caspase-8, monoclonal cytochrome c, monoclonal COX IV, polyclonal p-Rb, monoclonal Rb, polyclonal XIAP, monoclonal p-p38, polyclonal p38, polyclonal p-JNK (Thr183/Tyr185), monoclonal polyclonal JNK, p-Akt (Ser473) and β-actin were purchased from Cell Signaling Technology. The horseradish peroxidase (HRP)-labeled anti-mouse and anti-rabbit secondary antibodies were purchased from Santa Cruz. Bradford reagent was purchased from Bio-rad. Mitochondrial/cytosol fractionation kit was purchased from BioVision.