Scrambled control sirna

Manufactured by Santa Cruz Biotechnology

Sourced in United States, Germany

Scrambled control siRNA is a non-targeting siRNA sequence that does not have any known homology to genes in the human, mouse, or rat genomes. It can be used as a negative control in RNA interference experiments to determine the extent of non-specific effects that may occur with siRNA treatment.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Control siRNA (510 citations) Scrambled siRNA (158 citations) SiRNAs (95 citations) Scrambled control siRNA (sc-37007) (6 citations) Scrambled control (4 citations) Control scrambled siRNA (sc37007) (3 citations) Scrambled (Control-A) and siRNA sequences (2 citations)

78 protocols using scrambled control sirna

Modulation of ATF4 and CEBPB in MDA-MB-231 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

With a 70–80% confluence in 6 well plates, MDA-MB-231 cells were transfected using the siRNA transfection reagent (Santa cruz) per manufacturer’s instructions. siRNAs used in the transient transfections were: control scrambled siRNA (Santa cruz, sc-37007), CEBPB siRNA (Santa cruz, sc-44251), ATF4 siRNA (Santa cruz, sc-35112) and PERK siRNA (Santa cruz, sc-36213). Following siRNA treatment for 48 hr, cells were treated with YW3-56. To analyze the effects of siRNA treatment on YW3-56 mediated cell killing, control scrambled siRNA, ATF4 siRNA, SESN2 siRNA (Santa cruz, sc-106544), and DDIT4 siRNA (Santa cruz, sc-45806) were transfected into 1833 cells. At 24 hr following transfection, YW3-56 was added to treat the cells and cell growth was analyzed 48 hr later. To overexpress ATF4 or CEBPB, with a 70–80% confluence in 6-well plate, MDA-MB-231 cells were transfected with pRK-ATF4 (Addgene, 26114) plasmid or pCMV-CEBPB (Origene, sc319561) plasmid. Empty pRK and pIRES plasmids were used as negative control. At 24 hr after forced expression of ATF4 and CEBPB, qRT-PCR and Western blot were performed to analyze the effects of ATF4 and CEBPB on gene expression.

Wang S., Chen X.A., Hu J., Jiang J.K., Li Y., Chan-Salis K.Y., Gu Y., Chen G., Thomas C., Pugh B.F, & Wang Y. (2015). ATF4 Gene Network Mediates Cellular Response to the Anticancer PAD Inhibitor YW3-56 in Triple Negative Breast Cancer Cells. Molecular cancer therapeutics, 14(4), 877-888.

+ Open protocol

+ Expand

Silencing VLK/PKDCC in Human TM Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Small interfering RNA (siRNA) directed against human VLK/PKDCC (C. No. sc-94454) and a corresponding, scrambled control siRNA (C. No. sc-169299) were purchased from Santa Cruz Biotechnology (Dallas, TX). Human TM cells grown in plastic 6-well plates or petri- dishes were transfected using 25 pmol human VLK siRNA (containing a mixture of three sets of interfering short RNAs) or control siRNA, the Lipofectamine RNAiMax reagent (C. No. 13778-075, Invitrogen/ThermoFisher, Waltham, MA) and Opti-MEM media (C. No. 319850-70) supplemented with antibiotics per manufacturer’s instructions. Briefly, 3ml Opti-MEM media containing RNAiMax reagent and the respective siRNAs were added to cells grown in culture dishes and incubated for 2 h in a humidified cell culture incubator, following which 2 ml of 10% serum containing DMEM media was added slowly. After 72 h following transfection, cells were photographed using a phase contrast microscope (Zeiss AX10) to monitor for morphological changes. Media, cells and ECM protein fractions were collected from these cultures, and processed to analyze the levels of VLK and TyrP proteins using appropriate antibodies. The volume of transfection Opti-MEM media (containing RNAiMax reagent and respective siRNAs) was adjusted accordingly for cells grown on glass cover slips and transfections were performed as mentioned above.

Maddala R., Skiba N.P, & Rao P.V. (2017). Vertebrate Lonesome Kinase Regulated Extracellular Matrix Protein Phosphorylation, Cell Shape and Adhesion in Trabecular Meshwork Cells. Journal of cellular physiology, 232(9), 2447-2460.

+ Open protocol

+ Expand

Silencing PI3K and MEK in LLC-PK Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

LLC-PK cells grown to 70% to 80% confluence were transfected with reported siRNAs against PI3K p85α or MEK (Santa Cruz) or scrambled control siRNA (Santa Cruz) (22 (link)) by use of Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. To optimize the knockdown efficiency, a second transfection was carried out 24 h after the first transfection, and subsequent experiments were performed 48 h later. To confirm siRNA knockdown of each target protein, LLC-PK cells treated in parallel were analyzed by Western blotting as described below.

Soliman M., Kim D.S., Park J.G., Kim J.Y., Alfajaro M.M., Baek Y.B., Cho E.H., Park C.H., Kang M.I., Park S.I, & Cho K.O. (2018). Phosphatidylinositol 3-Kinase/Akt and MEK/ERK Signaling Pathways Facilitate Sapovirus Trafficking and Late Endosomal Acidification for Viral Uncoating in LLC-PK Cells. Journal of Virology, 92(24), e01674-18.

+ Open protocol

+ Expand

Beclin 1 Knockdown and Resveratrol Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Beclin 1 (catalog no. sc-29797) and scrambled control siRNA (catalog no. sc-37007) were purchased from Santa Cruz Biotechnology, Inc. The Beclin 1 siRNA sequences were as follows: Forward, 5′-CAGCUCAACGUCACUGAAATT-3′ and reverse, 5′-UUUCAGUGACGUUGAGCUGTT-3′. The control siRNA sequences were not available. Briefly, 100 nM of Beclin 1 or control siRNA was transfected into cells using Lipofectamine^® 2000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.), according to the manufacturer's protocol, and non-transfected cells were set as a blank control. After 24 h, cells were treated with 40 µM Res for an additional 48 h and subsequently analyzed via western blotting as previously described.

Yao H., Fan M, & He X. (2020). Autophagy suppresses resveratrol-induced apoptosis in renal cell carcinoma 786-O cells. Oncology Letters, 19(4), 3269-3277.

+ Open protocol

+ Expand

siRNA Transfection for KLF5 and HIF-1α

Check if the same lab product or an alternative is used in the 5 most similar protocols

KLF5 siRNA, and scrambled control siRNA were purchased from OriGene (Rockville, MD, USA). HIF-1α siRNA, and scrambled control siRNA were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Cells were transfected using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) for 6 h, following which the lipid and siRNA complex was removed and fresh growth medium was added. After 48 h of transfection, cells were used for immunoblotting.

Pratheeshkumar P., Siraj A.K., Divya S.P., Parvathareddy S.K., Siraj S., Diaz R., Begum R., Al-Sobhi S.S., Al-Dayel F, & Al-Kuraya K.S. (2021). Prognostic Value and Function of KLF5 in Papillary Thyroid Cancer. Cancers, 13(2), 185.

+ Open protocol

+ Expand

Overexpression of B7-H4 in Bladder Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

Full-length human B7-H4 cDNA was obtained from Origene Technologies (Rockville, MD, USA) and cloned into the pcDNA3.1(+) expression vector. B7-H4-targeting siRNA and scrambled control siRNA were purchased from Santa Cruz Biotechnology. The pcDNA3.1/B7-H4 plasmid or empty vector was transfected into bladder cancer cells 24 h after seeding using Lipofectamine 3000 transfection reagent (Invitrogen) according to the manufacturer’s protocol. Transfections of individual siRNAs (40 nM) were performed using DharmaFECT transfection reagents (Dharmacon, Lafayette, CO, USA). In some experiments, T24 cells were pretreated with SN50 (10 μM; Calbiochem, San Diego, CA, USA)12 (link) for 30 min before transfection with the pcDNA3.1/B7-H4 plasmid. For generation of stable cell clones, cells transfected with the pcDNA3.1/B7-H4 plasmid or empty vector were selected in the presence of 800 μg/ml G418 (Sigma-Aldrich, St. Louis, MO, USA).

Wu H., Wang X., Mo N., Zhang L., Yuan X, & Lü Z. (2018). B7-Homolog 4 Promotes Epithelial–Mesenchymal Transition and Invasion of Bladder Cancer Cells via Activation of Nuclear Factor-κB. Oncology Research, 26(8), 1267-1274.

+ Open protocol

+ Expand

Modulating Autophagy and Akt Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Knockdown of gene expression was conducted using small interfering RNA (siRNA) targeting Human LC3 and JNK, as well as scrambled control siRNA (Santa Cruz Biotechnology). Expression of Akt was augmented through transient transfection of constitutively active myristoylated Akt (Myr-Akt) construct, kindly provided by Professor Marc Peters-Golden (University of Michigan, Ann Arbor, MI, USA). Transfection of U251 cells with siRNA (100 nM) and plasmids (1 ng/µL, Myr-Akt and control construct) using Lipofectamine 2000 (2.8 µg/mL) (Thermo Fisher Scientific, Waltham, MA, USA) was performed according to the manufacturer’s recommendations. Cells were allowed to grow for 24 h following transfection and treated as described in figure legends.

Despotović A., Janjetović K., Zogović N, & Tovilović-Kovačević G. (2023). Pharmacological Akt and JNK Kinase Inhibitors 10-DEBC and SP600125 Potentiate Anti-Glioblastoma Effect of Menadione and Ascorbic Acid Combination in Human U251 Glioblastoma Cells. Biomedicines, 11(10), 2652.

+ Open protocol

+ Expand

miR-21 and PDCD4 Regulation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

miR-21 precursor and negative control precursor miRNA were purchased from GenePharma (Shanghai, China). PDCD4 small interfering RNA (siRNA) and scrambled control siRNA were obtained from Santa Cruz Biotechnology. They were similarly transfected using Lipofectamine 2000 (Invitrogen, San Diego, CA, USA).

Zhu Y., Liu L., Hu L., Dong W., Zhang M., Liu Y, & Li P. (2019). Effect of Celastrus orbiculatus in inhibiting Helicobacter pylori induced inflammatory response by regulating epithelial mesenchymal transition and targeting miR-21/PDCD4 signaling pathway in gastric epithelial cells. BMC Complementary and Alternative Medicine, 19, 91.

+ Open protocol

+ Expand

Gene Silencing in SK-N-SH Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human HuD-, PKCε-, and NEP-specific siRNA and scrambled control siRNA were purchased from Santa Cruz. The gene silencing conditions for each gene were determined by electroporation using the Nucleofector system (Lonza) according to manufacturer's specifications. Briefly, SK-N-SH cells (1×10⁵ cells/ml) were harvested and resuspended in 100 µl of Nucleofector transfection solution. After transfection of HuD, PKCε, NEP, or scrambled control siRNA (20 nM), cells were immediately plated in dishes containing complete media. The following day cells were split, and 72 hr later either lysed or subjected to further analyses, as described.

Lim C.S, & Alkon D.L. (2014). PKCε Promotes HuD-Mediated Neprilysin mRNA Stability and Enhances Neprilysin-Induced Aβ Degradation in Brain Neurons. PLoS ONE, 9(5), e97756.

+ Open protocol

+ Expand

CHIP Knockdown in GBM8401 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

CHIP expression knockdown was conducted using specific small inhibitory RNAs (siR-NAs) according to the manufacturer’s protocol. Briefly, GBM8401 cells were transfected with CHIP siRNA into a pool of three siRNA duplexes (si-CHIP; sc-43555A, sc-43555B and sc-43555C) and a scrambled control siRNA (Santa Cruz Biotechnology, CA, USA). The siRNA transfection reagent used was Lipofectamine RNAiMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA) at 37 °C and 5% CO₂ for 72 h.

Sun L.W., Kao S.H., Yang S.F., Jhang S.W., Lin Y.C., Chen C.M, & Hsieh Y.H. (2021). Corosolic Acid Attenuates the Invasiveness of Glioblastoma Cells by Promoting CHIP-Mediated AXL Degradation and Inhibiting GAS6/AXL/JAK Axis. Cells, 10(11), 2919.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!