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Nomo 1

Manufactured by American Type Culture Collection
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Sourced in Germany

The NOMO-1 is a laboratory equipment designed for the cultivation and maintenance of microbial cultures. It provides a controlled environment for the growth and storage of a wide range of microorganisms, ensuring their viability and purity. The NOMO-1 offers precise temperature, humidity, and atmospheric conditions to support the specific requirements of different microbial strains.

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Lab products found in correlation

Molm 13, by American Type Culture Collection (5 mentions) Hl 60, by American Type Culture Collection (4 mentions) Thp 1, by American Type Culture Collection (3 mentions) Kasumi 1, by American Type Culture Collection (3 mentions) Molm 14, by American Type Culture Collection (2 mentions) Dna lab cell line testing, by Leibniz Institute DSMZ (2 mentions) Kcl22, by American Type Culture Collection (2 mentions) Mycoplasma test, by Lonza (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Oci aml2, by American Type Culture Collection (1 mentions) Daunorubicin dnr, by Selleck Chemicals (1 mentions) Cytarabine ara c, by Selleck Chemicals (1 mentions) Glutamine, by Thermo Fisher Scientific (1 mentions) Mv4 11, by American Type Culture Collection (1 mentions) Oci aml3, by American Type Culture Collection (1 mentions) 293t cell line, by American Type Culture Collection (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)

7 protocols using nomo 1

1

Characterization of AML Cell Lines

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The AML cell lines U937, MOLM-14, MV4-11, HL60, HEL, THP-1, NOMO-1, OCI-AML2, OCI-AML3 and NB4 were provided by collaborators or were purchased from ATCC or DSMZ (Braunschweig, Germany). All cell lines were cultured in RPMI-1640 medium containing 10% FBS (GIBCO, Life Technologies, Carlsbad, CA, USA), 2 μM l−1 glutamine, 100 U mL−1 penicillin, and 100 μg ml−1 streptomycin (GIBCO, Life Technologies, Carlsbad, CA, USA). The 293T cell line was purchased from ATCC and cultured in DMEM containing 10% FBS (GIBCO, Life Technologies, Carlsbad, CA, USA), 2 μM l−1 glutamine, 100 U mL−1 penicillin, and 100 μg mL−1 streptomycin (GIBCO, Life Technologies, Carlsbad, CA, USA). Daunorubicin (DNR) and cytarabine (ARA-C) were purchased from Selleck Chemicals LLC (Houston, TX, USA) and Sigma-Aldrich (St. Louis, MO, USA), respectively; SIRT6 chemical inhibitor [2,4-dioxo-N-(4-(pyridin-3-yloxyphenyl)-1,2,3,4-tetrahydroquinazoline-6-sulfonamide, henceforth named compound 1] was obtained from MolPort (Riga, Latvia).
Cagnetta A., Soncini D., Orecchioni S., Talarico G., Minetto P., Guolo F., Retali V., Colombo N., Carminati E., Clavio M., Miglino M., Bergamaschi M., Nahimana A., Duchosal M., Todoerti K., Neri A., Passalacqua M., Bruzzone S., Nencioni A., Bertolini F., Gobbi M., Lemoli R.M, & Cea M. (2018). Depletion of SIRT6 enzymatic activity increases acute myeloid leukemia cells’ vulnerability to DNA-damaging agents. Haematologica, 103(1), 80-90.
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2

THP1 Cell Culture and Synchronization

Cited 1 time
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THP1 cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 2 mM l-glutamine, streptomycin (100 μg/ml), and penicillin (100 U/ml) at 37°C in 5% CO2. SS or G0 THP1 cells were prepared by washing with phosphate-buffered saline (PBS) followed by serum starvation at a density of 2 × 105 cells/ml. AraC-treated cells were prepared by treatment with indicated concentrations of AraC for indicated periods of time. THP1 (TIB-202) and monocytes (CRL9855) were obtained from the American Type Culture Collection (ATCC). NOMO1 and MOLM13 were obtained from ATCC and from the Scadden group (1 (link)). Cell lines were tested for mycoplasma (Promega) and authenticated by the ATCC Cell Authentication Testing Service (1 (link)).
Datta C., Truesdell S.S., Wu K.Q., Bukhari S.I., Ngue H., Buchanan B., Le Tonqueze O., Lee S., Kollu S., Granovetter M.A., Boukhali M., Kreuzer J., Batool M.S., Balaj L., Haas W, & Vasudevan S. (2022). Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells. Science Advances, 8(43), eabo1304.
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3

Cell Line Authentication and Maintenance

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U937, HL-60, MOLM13, UCSD-AML1, NB4, NOMO-1, and 293T cells were purchased from ATCC. CMK-86 cells were purchased from JCRB Cell Bank. EOL-1 were purchased from DSMZ. MV4–11, MOLM14 and THP-1 cells were provided by Scott Armstrong. MOLM13 and MOLM14 cells were originally derived from the same patient. All cell lines were maintained in RPMI 1640 (Cellgro) supplemented with 1% penicillin/streptomycin (PS; Cellgro) and 10% FBS (Sigma-Aldrich) at 37°C with 5% CO2. The 293T cells were maintained in Dulbecco’s modified Eagle’s medium (Invitrogen) supplemented with 10% FBS (Invitrogen) and 1% PS. All cell lines were tested negative for Mycoplasma and were authenticated using short tandem repeat (STR) profiling.
Cremer A., Ellegast J.M., Alexe G., Frank E.S., Ross L., Chu S.H., Pikman Y., Robichaud A., Goodale A., Häupl B., Mohr S., Rao A.V., Walker A.R., Blachly J.S., Piccioni F., Armstrong S.A., Byrd J.C., Oellerich T, & Stegmaier K. (2019). Resistance mechanisms to SYK inhibition in acute myeloid leukemia. Cancer discovery, 10(2), 214-231.
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4

Cell Line Authentication and Mycoplasma Testing

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MOLM13, KCL-22, Kasumi-1, K562, THP-1, U937, TF-1, NB4, HL-60, KG-1, Nomo-1, and 293T cell lines, purchased from ATCC and DSMZ (Supplementary Table 16), were authenticated by Genetica DNA Lab - Cell Line Testing (www.celllineauthentication.com). Cells were routinely tested and confirmed negative for mycoplasma in house by using a Mycoplasma Test from Lonza Biosciences (#LT07-218).
Prieto C., Nguyen D.T., Liu Z., Wheat J., Perez A., Gourkanti S., Chou T., Barin E., Velleca A., Rohwetter T., Chow A., Taggart J., Savino A.M., Hoskova K., Dhodapkar M., Schurer A., Barlowe T.S., Vu L.P., Leslie C., Steidl U., Rabadan R, & Kharas M.G. (2021). Transcriptional control of CBX5 by the RNA binding proteins RBMX and RBMXL1 maintains chromatin state in myeloid leukemia. Nature cancer, 2, 741-757.
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5

Modulating Membrane-Bound and Soluble MSLN

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Nomo-1 and Kasumi-1 cell lines were obtained from ATCC and maintained per manufacturer's instructions. We engineered Kasumi-1 MSLN+ cell line by transducing Kasumi-1 cells with a lentivirus containing the MSLN transgene driven by the EF1a promoter (see ref. 7 (link)). Jurkat Nur77 reporter cells were maintained in RPMI supplemented with 20% FBS and 2 mmol/L l-Glutamine.
GM6001 (Selleckchem, catalog no. S7157) is a matrix metalloprotease inhibitor. It is stable in vitro, but its half-life of in vivo is reported to be <15 minutes (ref. 14 (link); and see details at GM6001-MMP-Inhibitor,MM_NF-CC1010" xlink:show="new">https://www.emdmillipore.com/US/en/product/GM6001-MMP-Inhibitor,MM_NF-CC1010). Nomo-1 cells were treated with GM6001 (50 μmol/L) or DMSO control for 48 hours prior to evaluation of surface MSLN by flow cytometry; of soluble MSLN in the culture supernatant by ELISA; and of cell lysis activity by coculture with MSLN CAR T cells. For in vitro cytotoxicity assay, fresh GM6001 (50 μmol/L) or DMSO was added to the coculture of Nomo-1 and CAR T cells. For in vivo assays, mice were treated with GM6001 at 100 mg/kg once a day for 3–7 days via intraperitoneal injection.
Le Q., Castro S., Tang T., Loeb A.M., Hylkema T., McKay C.N., Perkins L., Srivastava S., Call L., Smith J., Leonti A., Ries R., Pardo L., Loken M.R., Correnti C., Fiorenza S., Turtle C.J., Riddell S., Tarlock K, & Meshinchi S. (2021). Therapeutic Targeting of Mesothelin with Chimeric Antigen Receptor T Cells in Acute Myeloid Leukemia. Clinical Cancer Research, 27(20), 5718-5730.
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6

Antiproliferative Assays of AML Cell Lines

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The human AML cell lines were obtained from ATCC, except that EOL-1, MOLM-13, MOLM-14, MUTZ-8, NOMO-1 and SKM-1 were obtained from DSMZ (RRID:CVCL_0098). The mouse Ba/F3 cell lines expressing FLT3 mutants ITD, D835Y, ITD plus D835Y, ITD plus F691L were a generous gift from Dr. Michael Andreeff at the MD Anderson Cancer Center, Houston, TX. All lines were STR authenticated at ATCC or DSMZ. Mycoplasma testing was conducted every 2 months using the Universal Mycoplasma Detection Kit from ATCC (catalog# 30–1012K). The last mycoplasma test was within 2 months of the last experiment with any given cell line. The cells were cultured in the medium recommended by the source for each cell line. Antiproliferative assays were performed using Cell Titer 96 Aqueous One Solution Cell Proliferation assay (Promega, catalogue number G3581). Cells were exposed in four replicates to increasing concentrations of drugs for 72 h. Studies using Ba/F3 cells expressing wild type and mutant forms of BTK were performed by Advanced Cellular Dynamics, Inc, Seattle, WA.
Rice W.G., Howell S.B., Zhang H., Rastgoo N., Local A., Kurtz S.E., Lo P., Bottomly D., Wilmot B., McWeeney S.K., Druker B.J, & Tyner J.W. (2022). Luxeptinib (CG-806) Targets FLT3 and Clusters of Kinases Operative in Acute Myeloid Leukemia. Molecular Cancer Therapeutics, 21(7), 1125-1135.
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7

Cell Line Authentication and Mycoplasma Testing

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MOLM13, KCL-22, Kasumi-1, K562, THP-1, U937, TF-1, NB4, HL-60, KG-1, Nomo-1, and 293T cell lines, purchased from ATCC and DSMZ (Supplementary Table 16), were authenticated by Genetica DNA Lab - Cell Line Testing (www.celllineauthentication.com). Cells were routinely tested and confirmed negative for mycoplasma in house by using a Mycoplasma Test from Lonza Biosciences (#LT07-218).
Prieto C., Nguyen D.T., Liu Z., Wheat J., Perez A., Gourkanti S., Chou T., Barin E., Velleca A., Rohwetter T., Chow A., Taggart J., Savino A.M., Hoskova K., Dhodapkar M., Schurer A., Barlowe T.S., Vu L.P., Leslie C., Steidl U., Rabadan R, & Kharas M.G. (2021). Transcriptional control of CBX5 by the RNA binding proteins RBMX and RBMXL1 maintains chromatin state in myeloid leukemia. Nature cancer, 2, 741-757.
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