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Anti muc5ac

Manufactured by Santa Cruz Biotechnology
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Anti-MUC5AC is an antibody product offered by Santa Cruz Biotechnology. It is designed to detect the MUC5AC protein, which is a mucin glycoprotein. The core function of this antibody is to specifically bind to and identify the presence of MUC5AC in various biological samples.

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Lab products found in correlation

4 phenylbutyric acid 4 pba, by Merck Group (1 mentions) Anti akt1, by Santa Cruz Biotechnology (1 mentions) Anti pi3k p85α, by Santa Cruz Biotechnology (1 mentions) Anti chop, by Abcam (1 mentions) Anti il 13, by Abcam (1 mentions) Anti phospho lyn, by Cell Signaling Technology (1 mentions) Anti il 13, by R&D Systems (1 mentions) Af 413 na, by R&D Systems (1 mentions) Anti nf κb p65, by Santa Cruz Biotechnology (1 mentions) Anti phospho nf κb p65, by Santa Cruz Biotechnology (1 mentions) Anti histone h3, by Abcam (1 mentions) Il 13, by Thermo Fisher Scientific (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti lyn, by Santa Cruz Biotechnology (1 mentions) Anti bip, by Abcam (1 mentions) Histofine sab po r kit, by Nichirei Biosciences (1 mentions) Simple stain max po kit, by Nichirei Biosciences (1 mentions) Anti acetylated alpha tubulin, by Merck Group (1 mentions) Lcm 510 confocal microscope, by Zeiss (1 mentions) Sc 20119, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

MUC5AC (8 citations) Anti-MUC5AC (sc-21701) (2 citations) Mouse anti-Muc5ac (2 citations)

5 protocols using anti muc5ac

1

Antibody-Based Histology and Cell Staining Protocols

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The following antibodies for histology and cell staining were purchased from Santa Cruz Biotechnology: anti-MUC5AC (Santa Cruz, CA, sc-16903, AB_649616), anti-phospho-PI3K p85α (Tyr467: Santa Cruz, CA, sc-293115, AB_10844180), anti-PI3K p85α (Santa Cruz, CA, sc-31970, AB_2268186), anti-phospho-Akt1 (Thr308: Santa Cruz, CA, sc-135650, AB_2224730), anti-Akt1 (Santa Cruz, CA, sc-1618, AB_630849), anti-phospho-NFκB p65 (Ser536: Santa Cruz, CA, sc-33020, AB_2179018), anti-NFκB p65 (Santa Cruz, CA, sc-109, AB_632039), anti-Lyn (Santa Cruz, CA, sc-15, AB_2281450), and anti-β-actin (Santa Cruz, CA, sc-130656, AB_2223228). The following antibodies for histology were purchased from Abcam Biotechnology: anti-BIP (Abcam, ab21685, AB_2119834), anti-CHOP (Abcam, ab11419, AB_298023), anti-histone H3 (Abcam, ab1791, AB_302613), and anti-IL-13 (Abcam, ab133353, AB_11157609). Anti-phospho-Lyn (Tyr416: Cell Signaling Technology, #2101, AB_331697) was purchased from Cell Signaling Technology. Anti-IL13 (R&D Systems, AF-413-NA, and AB_2124173) and IL-13 ELISA reagents (R&D Systems, M1300CB) were purchased from R&D Systems. IL-13 (PeproTech, #200-13), 4-Phenylbutyric acid (4-PBA, Sigma-Aldrich, P21005), PI3K Inhibitor PI-103 (Selleck, S1038) were purchased as indicated. A nonsilencing siRNA control and a Lyn-specific siRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).
Wang X., Yang X., Li Y., Wang X., Zhang Y., Dai X., Niu B., Wu J., Yuan X., Xiong A., Liu Z., Zhong N., Wu M, & Li G. (2016). Lyn kinase represses mucus hypersecretion by regulating IL-13-induced endoplasmic reticulum stress in asthma. EBioMedicine, 15, 137-149.
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2

Histological and Immunohistochemical Analysis of Tumor Tissues

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For light microscopic analysis, formaldehyde-fixed, paraffin-embedded tumor tissues were stained with hematoxylin and eosin (H&E) using standard techniques. Immunohistochemical staining was performed using the Simple Stain MAX-PO kit (Nichirei Bioscience), the Histofine SAB-PO (R) kit (Nichirei Bioscience) or the BenchMark GX automated slide preparation system (Ventana). The following primary antibodies were used: anti-MUC5AC (Santa Cruz Biotechnology), anti-ETV4 (Abcam), anti-CCND2 (Santa Cruz Biotechnology).
Yoshimoto T., Tanaka M., Homme M., Yamazaki Y., Takazawa Y., Antonescu C.R, & Nakamura T. (2017). CIC-DUX4 induces small round cell sarcomas distinct from Ewing sarcoma. Cancer research, 77(11), 2927-2937.
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3

Immunostaining of Airway Epithelial Cells

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Example 10

The ALI culture was washed with PBS and fixed by 4% PFA for 20 mins. at room temperature. The fixed structures were washed with PBS and blocked by TBST (Tris Buffered Saline with Tween 20) with 0.1% Triton and 5% BSA for one hour. The structures were then incubated with the primary antibody, e.g., anti-acetylated alpha tubulin (Sigma) and anti-Muc5Ac (Santa Cruz), at 1:500 dilution for one hour, washed with PBS and then incubated with the Alexa Fluro® 488 donkey anti-mouse IgG (H+L) and Alexa Fluro® 594 donkey anti-rabbit IgG (H+L) secondary antibodies for one hour. After washing, the VECTASHIELD mounting medium was added, and 3D images were taken by Zeiss LCM510 confocal microscope.

US09964535B2. Treatment of inflammatory diseases (2018-05-08). The Jackson Laboratory [US]. Inventors: Wa Xian [US].
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4

Immunohistochemical Staining of MUC5B and MUC5AC

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For immunohistochemical staining, primary antibodies were used at the following concentrations: rabbit polyclonal anti-MUC5B (1:200; sc-20 119, Santa Cruz Biotechnology) and rabbit polyclonal anti-MUC5AC (1:50; sc-20 118, Santa Cruz Biotechnology).
Wakata K., Tsuchiya T., Tomoshige K., Takagi K., Yamasaki N., Matsumoto K., Miyazaki T., Nanashima A., Whitsett J.A., Maeda Y, & Nagayasu T. (2015). A favourable prognostic marker for EGFR mutant non-small cell lung cancer: immunohistochemical analysis of MUC5B. BMJ Open, 5(7), e008366.
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5

Murine Asthma Model Protocol

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Luteolin, picrotoxin, ovalbumin and the PAS staining kit and other chemical reagents were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA). Anti-Muc5ac and anti-alpha-tubulin antibodies were purchased from Santa Cruz Biotechnology (sc-21701, sc-12462-R, Dallas, TX, USA). Horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Pierce Biotechnology (Thermo Fisher Scientific, Rockford, IL, USA). IL-4, IL-5 and IgE ELISA kits were purchased from BD Biosciences (San Diego, CA, USA). The IL-13 kit was purchased from R&D Systems (Minneapolis, MN, USA). OVA for intraperitoneal (i.p.) injection was adsorbed to aluminum hydroxide adjuvant (Santa Cruz, Dallas, TX, USA) at a ratio of 50 μg to 2 mg in 200 μl PBS. OVA for intratracheal (i.t.) injection was dissolved in saline at a final concentration of 2.5 mg/ml. Luteolin (Sigma-Aldrich, St. Louis, MO, USA) was prepared in DMSO and diluted with saline. picrotoxin (Sigma-Aldrich, St. Louis, MO, USA) was prepared in DMSO and diluted with saline. Saline with 0.5% DMSO was used as a vehicle in control groups. The final concentration of DMSO in all reaction mixtures was less than 0.5%.
Shen M.L., Wang C.H., Lin C.H., Zhou N., Kao S.T, & Wu D.C. (2016). Luteolin Attenuates Airway Mucus Overproduction via Inhibition of the GABAergic System. Scientific Reports, 6, 32756.
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