Sk n dz

Manufactured by Thermo Fisher Scientific

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The SK-N-DZ is a laboratory instrument designed for cell culture applications. It is a cell line derived from a human neuroblastoma. The core function of this product is to provide a stable and well-characterized cell line for research and experimentation purposes.

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5 protocols using sk n dz

Neuroblastoma Cell Line Transfection

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Neuroblastoma cell lines BE(2 (link))-C, CHP-212, SH-SY5Y, SK-N-AS and SK-N-DZ were obtained from the American Type Culture Collection. KELLY and NGP cells were were a gift from Dr. Raymond Stallings and were obtained originally from the European Collection of Animal Cell Cultures (Porton Down, UK) and Cancer Therapy and Research Centre, San Antonio, TX respectively. Cells were grown in DMEM/F12 (GIBCO) supplemented with 10% fetal bovine serum (GIBCO) and 1% pen/strep (GIBCO). HeLa cells were obtained from the American Type Culture Collection and were cultured in RPMI-1640 (GIBCO) supplemented with 10% FBS and 1% pen/strep. 293T cells were obtained from the American Type Culture Collection and were cultured in DMEM-High Glucose (HyClone) supplemented with 10% FBS and 1% pen/strep. Cells were passaged no more than fifteen times and were tested for mycoplasma contamination using DAPI staining (2 μg/mL) (Thermo Fisher). All cells were authenticated using short tandem repeat profiling.
SMARTpool siRNAs against the transcription factors were obtained from Dharmacon (Supplementary Table 1). Neuroblastoma cells (BE(2)-C, CHP-212, KELLY, NGP, SH-SY5Y, SK-N-AS, and SK-N-DZ) were reverse transfected into 96-wells with siRNAs using RNAiMAX (Thermo Fisher).

Kosti A., Du L., Shivram H., Qiao M., Burns S., Garcia J.G., Pertsemlidis A., Iyer V.R., Kokovay E, & Penalva L.O. (2019). ELF4 is a target of miR-124 and promotes neuroblastoma proliferation and undifferentiated state. Molecular cancer research : MCR, 18(1), 68-78.

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Hypoxic Neuroblastoma Cell Culture

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Neuroblastoma cell lines SK-N-BE2, SK-N-DZ, LAN-5, and LA1-55n were maintained in RPMI 1640 media (ThermoFisher Scientific) supplemented with 10% heat-inactivated fetal bovine serum. SK-N-DZ was purchased from ATCC. All cell lines were authenticated within six months of all experiments by short tandem repeat (STR) profiling and profiles were found to be identical to known profiles for the cell lines. All cell lines tested negative for Mycoplasma contamination using the MycoAlert detection assay (Lonza). Cells were seeded 18–24 hours prior to hypoxia exposure. For hypoxic conditions, cells were incubated in 1% O₂ and 5% CO₂. For normoxic conditions cells were incubated at 21% O₂ and 5% CO₂. All experiments were performed in triplicate.

Applebaum M.A., Jha A.R., Kao C., Hernandez K.M., DeWane G., Salwen H.R., Chlenski A., Dobratic M., Mariani C.J., Godley L.A., Prabhakar N., White K., Stranger B.E, & Cohn S.L. (2016). Integrative genomics reveals hypoxia inducible genes that are associated with a poor prognosis in neuroblastoma patients. Oncotarget, 7(47), 76816-76826.

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Culturing Human Neuroblastoma Cell Lines

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The human neuroblastoma cell lines SK-N-AS, BE(2 (link))C, SK-N-DZ, SK-N-F1 and SHEP1 were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). The BE(2 (link))C cells were cultured in a 1:1 mixture of Dulbecco's modified Eagle's medium (DMEM) and Ham's nutrient mixture F12 (DMEM/F12; Thermo Fisher Scientific, Inc., Waltham, MA, USA) with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Inc.) and 1% penicillin/streptomycin (P/S). SK-N-AS, SK-N-DZ, SK-N-F1 and SHEP1 cells were cultured in DMEM (Thermo Fisher Scientific, Inc.) with 10% FBS and 1% P/S. The 293FT cell line (ATCC) was cultured with DMEM (Gibco; Thermo Fisher Scientific, Inc.) containing 1% glutamine, glycine and pyruvate. All cells were incubated at 37°C in an incubator with 5% CO₂.

Pang Y., Pan L., Zhang Y, & Liu G. (2019). TP53BP2 decreases cell proliferation and induces autophagy in neuroblastoma cell lines. Oncology Letters, 17(6), 4976-4984.

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Neuroblastoma Cell Lines for Research

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The NB cell lines used for the experiments including SK-N-BE (2 (link)), SKNDZ and SH-SY5Y were obtained from American Type Culture Collection (ATCC). Kelly cell line was kindly provided by Dr Guoliang Qing from Department of Pathophysiology, School of Basic Medical Sciences, Wuhan University. MYCN is highly expressed in SK-N-BE (2 (link)), SKNDZ and Kelly cell lines and low expressed in SH-SY5Y. All four cell lines are representative, widely used NB cell lines with relatively high feasibility. SK-N-BE (2 (link)), SKNDZ and SH-SY5Y cell lines were routinely cultured with DMEM (C11995500BT, Gibco, USA) supplemented with 10% fetal bovine serum (10099-141C, Gibco, USA) and1% Penicillin/Streptomycin (C100C5, New cell & Molecular Biotech, China), Kelly cell line was grown in complete RPMI-1640 (C11875500BT, Gibco, USA) supplemented with 10% FBS and 1% Penicillin/Streptomycin. All short tandem repeat (STR) -certified cell lines were cultured at 37C and 5% CO2 for up to 6 months after resuscitation, with mycoplasma contamination detected regularly using MycoAlert (LT07-710, Lonza, Switzerland).

Chen J., Sun M., Chen C., Jiang B, & Fang Y. (2022). Identification of hub genes and their correlation with infiltration of immune cells in MYCN positive neuroblastoma based on WGCNA and LASSO algorithm. Frontiers in Immunology, 13, 1016683.

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Maintaining Human Neuroblastoma Cell Lines

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The human NB cell lines SK-N-AS (ATCC® CRL-2137™) and SK-N-DZ (ATCC® CRL2149™) were obtained from the American Type Culture Collection (ATCC, Rockville, MA, USA). Mycoplasma testing was performed using a Mycoplasma Detection kit (Beijing Solarbio Science & Technology Co., Ltd.). SK-N-AS and SK-N-DZ cells were maintained in Dulbecco’s modified Eagle’s medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS) and with 1% penicillin and streptomycin antibiotics.

Liu N., Yang C., Yang L., Li T., Gong M., Wang H., Zhang J., Zhao H., Zou L, & He X. (2022). Matrine induces autophagy in human neuroblastoma cells via blocking the AKT-mTOR pathway. Medical Oncology (Northwood, London, England), 39(11), 167.

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