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2 protocols using 2 2 dithiodipyridine

1

Comprehensive Cell Line Cultivation Protocol

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Human
cervical cancer cell line (HeLa), human
hepatoma cell line (RG), mouse breast cancer cell line (4T1), human
proximal tubular epithelial cell line (HK-2), human non-small cell
lung adenocarcinoma cell line (H1975), human oral epidermoid carcinoma
cell line (KB), and human breast cancer cell line (MCF-7) were obtained
from the Laboratory Center for Medical Science of Lanzhou University.
Dulbecco’s modified Eagle’s medium (DMEM), RPMI-1640,
and fetal bovine serum (FBS) were purchased from Gibco. Neonatal bovine
serum (NBS) was obtained from Sijiqing Biotech (Hangzhou, China).
All Fmoc-protected amino acids, 1-hydroxybenzotriazole (HOBT), O-benzotriazole-N,N,N′, and N′-tetramethyl-uronium-hexafluoro-phosphate (HBTU),
were purchased from GL Biochem (Shanghai, China). Rink amide MBHA
resin was obtained from Hecheng (China). Triisopropylsilane (TIS), N,N-diisopropylethylamine (DIEA), and trifluoroacetic
acid (TFA) were supplied by J&K (China). 1,2-Ethanedithiol and
2,2′-dithiodipyridine were purchased from Energy Chemical.
Propidium iodide (PI) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) were ordered from Sigma-Aldrich. Triton X-100 was obtained
from Solarbio (China).
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2

Synthesis and Characterization of Multifunctional Polymer Conjugates

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(3-Mercaptopropyl) trimethoxysilane (97%, J&K), 2,2'-dithiodipyridine (98%, Energy Chemical), poly(ethylene glycol) methyl ether (PEG-OH, Mn = 750 g/mol, 98%, Energy Chemical), thionyl chloride (97%, J&K), 2-mercaptoethylamine hydrochloride (MCA, 99%, Energy Chemical), 1-(2-aminoethyl)piperazine (AEPZ, 97%, Aldrich), diisopropanolamine (DIAD, 98%, Energy Chemical), phthalimide (97%, Energy Chemical), triphenylphosphine (99%, Energy Chemical), hydrazine hydrate (95%, J&K), triethylamine (TEA, 99%, Beijing Chemical Works), succinic anhydride (99%, J&K), N, N'-bis(acryloyl)cystamine (BAC, 98%, Fluka), β-CD ( > 95%, TCI) was dried at 70 °C under vacuum for 24 h before use. Cell counting kit-8 was purchased from Beyotime Biotechnology. DMEM (Corning) with 4.5 g /L glucose, l-glutamine, without sodium pyruvate was purchased from Beijing BioDee Biotechnology Co. Ltd. MCF-7 cell lines were purchased from the American type Culture Collection (ATCC, Rockville, MD). Other reagents were commercially obtained from Beijing Chem. Co. and used without further purification.
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