Anti pan 14 3 3

Manufactured by Merck Group

Anti-pan-14-3-3 is a laboratory reagent designed for the detection and quantification of 14-3-3 proteins, a family of regulatory proteins involved in diverse cellular processes. The product provides a standardized and reliable tool for researchers to study the expression and function of 14-3-3 proteins in their research applications.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (2 mentions) Cycloheximide (chx), by Santa Cruz Biotechnology (1 mentions) Recombinant glua1, by OriGene (1 mentions) Poly d lysine, by Santa Cruz Biotechnology (1 mentions) Site directed mutagenesis reagent, by Agilent Technologies (1 mentions) Anti glua1, by Abcam (1 mentions) Anti map2, by Thermo Fisher Scientific (1 mentions) Anti α tubulin, by Cell Signaling Technology (1 mentions) Protein a g bead, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by GenScript (1 mentions) Anti ha, by GenScript (1 mentions) Ionomycin, by Adipogen (1 mentions) Anti n cadherin, by Merck Group (1 mentions) Tetrodotoxin citrate ttx, by Cayman Chemical (1 mentions) Dulbecco s modified eagle medium, by Merck Group (1 mentions) Anti ub, by Merck Group (1 mentions) Anti ha, by Merck Group (1 mentions) Crl 1573, by American Type Culture Collection (1 mentions) Anti nedd4 2, by Cell Signaling Technology (1 mentions) Anti myc, by Merck Group (1 mentions)

2 protocols using anti pan 14 3 3

Epilepsy-Associated Nedd4-2 Mutant Characterization

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Dimethyl sulfoxide (DMSO) was from Fisher Scientific. AMPA was from Cayman Chemical and NBQX was from Alomone Labs. Recombinant GluA1 and 14-3-3ε were from Origene. Recombinant Nedd4-2 was from Abnova. R18 was from Sigma-Aldrich. Cycloheximide, poly-D-lysine and Protein A/G beads were from Santa Cruz Biotechnology. The antibodies used in this study were purchased from Santa Cruz Biotechnology (anti-α-Tubulin), Cell Signaling (anti-Nedd4-2, anti-pan-14-3-3, anti-N-cadherin and anti-Ubiquitin), Millipore (anti-GluA1), Abcam (anti-MAP2), Thermo Scientific (anti-HA) and GenScript Corporation (anti-Gapdh). The epilepsy-associated mutations were generated using site-directed mutagenesis reagent (Agilent) to introduce mutations into pCI-HA-Nedd4-2 [15 (link)]. The primers used are as below.
S233L: 5’-GGACGTGTCCTCGGAGTTGGACAATAACATCAGAC-3’,
5’-GTCTGATGTTATTGTCCAACTCCGAGGACACGTCC-3’;
E271A: 5’- GGGCGGGGATGTCCCCGCGCCTTGGGAGACCATTTC-3’,
5’- GAAATGGTCTCCCAAGGCGCGGGGACATCCCCGCCC-3’;
H515P: 5’- CGTTTGAAATTTCCAGTACCTATGCGGTCAAAGACATC-3’,
5’- GATGTCTTTGACCGCATAGGTACTGGAAATTTCAAACG-3’.

Zhu J., Lee K.Y., Jewett K.A., Man H.Y., Chung H.J, & Tsai N.P. (2017). Epilepsy-associated gene Nedd4-2 mediates neuronal activity and seizure susceptibility through AMPA receptors. PLoS Genetics, 13(2), e1006634.

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Investigating Protein Regulation in HEK293 Cells

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Dimethyl sulfoxide (DMSO) was from Thermo Fisher Scientific (catalog: BP231–100). Tetrodotoxin citrate (TTX) was from Cayman Chemicals (catalog: 14964). Ionomycin was from AdipoGen (catalog: AG-CN2–0416-M001). The antibodies used in this study were purchased from Cell Signaling (anti-Nedd4–2, RRID: AB_1904063; anti-pan-14-3-3, RRID: AB_10860606; anti-Myc, RRID: AB_490778; anti-N-Cadherin, RRID: AB_2798427; anti-HA, RRID: AB_10691311, and anti-Ub, RRID: AB_331292), Millipore (anti-GluA1-N-terminus, RRID: AB_1977459), Santa Cruz Biotechnology (anti-α-tubulin, RRID: AB_628411), and Proteintech (anti-Gapdh, RRID: AB_2107436), and ABclonal (anti-PPP3CA, RRID: AB_2758155). Human embryonic kidney (HEK 293) cells were from ATCC (ATCC #CRL-1573, RRID: CVCL_0045). HEK 293 cell line is not listed as a commonly misidentified cell line by the International Cell Line Authentication Committee (ICLAC) and was not authenticated in this study. Cells were cultured in Dulbecco’s Modified Eagle Medium (Sigma, catalog: 10017CV) with 10% Fetal Bovine Serum (JM Bioscience, catalog: 100–500). Cells were used between passages 4 to 25 and kept at 37°C in a humidified incubator containing 5% CO₂.

Zhu J., Lee K.Y., Jong T.T, & Tsai N.P. (2019). C2-lacking isoform of Nedd4-2 regulates excitatory synaptic strength through GluA1 ubiquitination-independent mechanisms. Journal of neurochemistry, 151(3), 289-300.

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