Flag m2 hrp a8592

Manufactured by Merck Group

Flag M2-HRP (A8592) is a laboratory product manufactured by Merck Group. It is used as a detection reagent in various immunoassay techniques. The core function of this product is to enable the detection and visualization of target analytes in biological samples.

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Lab products found in correlation

Trpc6, by Cell Signaling Technology (1 mentions) Glut4, by Merck Group (1 mentions) Anti rabbit igg hrp 7074, by Cell Signaling Technology (1 mentions) Ab202468, by Abcam (1 mentions) Ab154804, by Abcam (1 mentions) Ab214938, by Abcam (1 mentions) Ab169544, by Abcam (1 mentions) β actin hrp, by Abcam (1 mentions)

Spelling variants of this product

FLAG M2 (383 citations) A8592 (73 citations) Flag-HRP (41 citations) Flag (A8592) (4 citations) Flag-HRP (A8592) (3 citations) Horseradish peroxidase (HRP)-anti-Flag (M2) (A8592) (3 citations) Anti-FLAG M2-HRP (A8592; (2 citations)

2 protocols using flag m2 hrp a8592

Protein-Protein Interactions in Nox2 Signaling

Cited 1 time

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To analyze expression of endogenous Nox2 in mouse hearts and NRCMs, total membrane fraction was isolated as described previously^{36 (link)}. Mouse hearts were first powdered in liquid nitrogen and then lysed in hypotonic lysis buffer. Co-immunoprecipitation of TRPC6 with Flag-Nox2 and Myc-p22^phox was performed as previously described^{17 (link)}. Primary antibodies used were; GAPDH (sc-25778), gp91^phox (sc-130543), from Santa Cruz Biotechnology, Flag M2-HRP (A8592) from Sigma Aldrich, GFP (CHIP grade, ab290) from Abcam, Myc-tag (05–742) from Merck and TRPC6 from cell signaling technologies.

Oda S., Numaga-Tomita T., Kitajima N., Toyama T., Harada E., Shimauchi T., Nishimura A., Ishikawa T., Kumagai Y., Birnbaumer L, & Nishida M. (2017). TRPC6 counteracts TRPC3-Nox2 protein complex leading to attenuation of hyperglycemia-induced heart failure in mice. Scientific Reports, 7, 7511.

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Antibody Detection in Metabolic Pathways

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Antibodies against GLUT-1 (07-1401) and GLUT-4 (07-1404) were obtained from Millipore. Antibodies against PKM2 (4053), HK2 (2867), LDHA (2012), FOXP1 (4402), anti-mouse IgG-HRP (7076), and anti-rabbit IgG-HRP (7074) were obtained from Cell Signaling Technology. Antibodies against pyruvate dehydrogenase kinase 4 (PDK4) (ab214938), pyruvate dehydrogenase kinase 1 (PDK1) (ab202468), aldolase (ALDOA) (ab169544), phosphofructokinase (PFKM) (ab154804), glutamate dehydrogenase 1 (GLUD1) (ab166618), and β-ACTIN-HRP (ab8226) were from Abcam.
Antibodies against myosin (skeletal, slow type I) (M8421), FLAG–M2-HRP (A8592), and FLAG (F7425) were obtained from Sigma-Aldrich. Vector M.O.M. Immunodetection Kit (PK-2200) was obtained from Vector Laboratories.

Ma H., Sukonina V., Zhang W., Meng F., Subhash S., Palmgren H., Alexandersson I., Han H., Zhou S., Bartesaghi S., Kanduri C, & Enerbäck S. (2023). The transcription factor Foxp1 regulates aerobic glycolysis in adipocytes and myocytes. The Journal of Biological Chemistry, 299(6), 104795.

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