The phospho-p38 (D3F9) antibody from Cell Signaling Technologies was used to detect Hog1-P67 . HA-tagged Rim101 was detected using the anti-HA antibody [HA.C5] from Abcam (ab18181). The anti-TAP antibody (CAB1001) was from ThermoFisher Scientific. Anti-Rps5 is from ProteinTech (16964-1-AP). Polyclonal antibodies were gifts from M. Seedorf (Rps3), G. Dieci (Rps8)68 , L. Valášek (Rps0) and A. Link (Asc1). The monoclonal antibody against Tub1, developed by J. Frankel, was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa. The Fap7 and Rcl1 antibodies were raised against purified recombinant proteins by Josman, Llc. Rps10 and Rps26 antibodies were raised against peptides from each protein by New England Peptide. All four antibodies were tested against yeast lysates and either recombinant protein or purified 40S ribosomal subunits.
Anti tap antibody cab1001
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Anti-TAP antibody (CAB1001) is a laboratory reagent used to detect and purify Tandem Affinity Purification (TAP) tagged proteins. It specifically recognizes the TAP tag, which is a fusion tag commonly used for protein purification and complex identification. The antibody can be used in various immunological techniques, such as Western blotting, immunoprecipitation, and affinity chromatography, to identify and isolate TAP-tagged proteins from cell lysates or other biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Phospho p38 d3f9 antibody, by Cell Signaling Technology (2 mentions)
Anti ha antibody ha c5, by Abcam (2 mentions)
Anti rps5, by Proteintech (2 mentions)
Methyl methanesulfonate, by Merck Group (2 mentions)
Camptothecin, by Thermo Fisher Scientific (2 mentions)
Dihydroethidium dhe, by Thermo Fisher Scientific (2 mentions)
Hrp conjugated anti rabbit antibody, by Cell Signaling Technology (2 mentions)
Hydroxyurea, by Merck Group (2 mentions)
Paraquat, by Merck Group (2 mentions)
Dihydrorhodamine 123 dhr, by Thermo Fisher Scientific (2 mentions)
Anti goat hrp conjugated antibody a16142, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 488 conjugated goat anti mouse antibody a11001, by Thermo Fisher Scientific (2 mentions)
Anti sod1 antibody ab 16831, by Abcam (2 mentions)
Anti pgk1 antibody ab 113687, by Abcam (2 mentions)
Protease and phosphatase phosstop inhibitor cocktails, by Roche (2 mentions)
Zeocin, by Thermo Fisher Scientific (2 mentions)
Hydrogen peroxide, by Merck Group (2 mentions)
Enhanced chemiluminescence method, by Thermo Fisher Scientific (1 mentions)
Anti g6pdh antibody, by Merck Group (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
5 protocols using anti tap antibody cab1001
1
Antibody detection of yeast proteins
2
Oxidative and DNA-damaging Agents Protocol
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Oxidative and DNA-damaging Agents Protocol
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Oxidative and DNA-damage drugs hydroxyurea (HU), methyl methanesulfonate (MMS), 4-Nitroquinoline N-oxide (4NQO), menadione, paraquat and hydrogen peroxide were purchased from Sigma-Aldrich. Zeocin was purchased from Invitrogen, and camptothecin (CPT) was a gift from Dr. Leroy Liu of the University of Medicine and Dentistry of New Jersey. Dihydrorhodamine 123 (DHR) and dihydroethidium (DHE) were purchased from Life Technology. Mouse anti-Myc (9E10) antibody (#200613) was purchased from Harlan Laboratories. Mouse anti-GST antibody (#2624), anti-mouse horseradish peroxidase (HRP)-conjugated antibody (#7076) and anti-rabbit HRP-conjugated antibody (#7074) were purchased from Cell Signaling Technology. Anti-goat HRP-conjugated antibody (A16142) and Alexa Fluor 488-conjugated goat anti-mouse antibody (A11001) were purchased from Life Technologies. Anti-Sod1 antibody (ab-16831) and anti-PGK1 antibody (ab-113687) were purchased from Abcam. Anti-Nop1 antibody (MCA28F2) was purchased from EnCor Biotechnology. Anti-TAP antibody (CAB1001) was purchased from Thermo Scientific. Protease and phosphatase (PhosSTOP) inhibitor cocktails were purchased from Roche.
4
Antibody detection of yeast proteins
5
Western Blot Protein Quantification
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Total cell lysates were extracted using the NaOH lysis/TCA precipitation/urea procedure. For western blot analyses, total protein was separated by SDS-PAGE and then transferred to a Merck Millipore PVDF membrane in transfer buffer (25 mM Tris base, 192 mM glycine, 20% methanol). After blocking with the blocking solution (1% Casein, 1X PBST, 0.1% Tween 20), the membrane was probed with the designated primary antibodies-anti-G6PDH antibody (Sigma, St. Louis, MO, USA; 1:8000 dilution) and anti-TAP antibody (CAB1001, Thermo Fisher Scientific; 1:2000 dilution)-and then with secondary antibodies. Immunopositive bands were developed by means of the enhanced chemiluminescence method (Thermo Fisher Scientific) and visualized using Western Lightning Chemiluminescence Reagent (PerkinElmer, Waltham, MA, USA).
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