Tecnai g2 t20 s twin transmission electron microscope

TEM was performed to visualize CsgA fibrils, and to test the effect of the D-peptide inhibitors. CsgA was purified as described above, and 30 μM was incubated overnight, at 25°C, with 300 rpm shaking in a plate reader, with and without 300 μM D-peptides (1:10 molar ratio). Five-microliter samples were then applied directly onto copper TEM grids with support films of Formvar/carbon (Ted Pella), which were charged by glow-discharge (PELCO easiGlow, Ted Pella) immediately before use. Grids were allowed to adhere for 2 min and negatively stained with 5 μl 2% uranyl acetate solution. Micrographs were recorded using a FEI Tecnai G2 T20 S-Twin transmission electron microscope at an accelerating voltage of 200 KeV, or using a FEI Tecnai T12 G2 transmission electron microscope operated at an accelerating voltage of 120 kV.

Samples were mounted on glow discharge-treated carbon type-B grids, and imaged using an FEI Tecnai G2 T20 S-Twin transmission electron microscope (TEM) at an accelerating voltage of 200 kV. Bacteria-containing samples were dehydrated before imaging, via incubation in glutaraldehyde solution (2% in PBS) for 16 h at 4 °C and subsequent treatment with a series of ethanol in water solutions of 10, 25, 50, 75, and 100 vol.%. Elemental mapping was performed using a Titan Themis (Thermo Fisher/FEI) scanning transmission electron microscope (STEM) operated at 200 kV and equipped with a high-angle annular dark-field (HAADF) and Bruker Dual-X EDX detectors.

Pristine and E-HNTs were mounted on a
carbon type-B grid and imaged using an FEI Tecnai G2 T20 S-Twin transmission
electron microscope coupled with an energy-dispersive X-ray (EDX)
detector at an accelerating voltage of 200 keV. EDX results were processed
using TIA (TEM Imaging & Analysis) software version 4.12, FEI
Company, OR, USA.