Perfusion fixed mouse brains were removed from skulls and cryo-protected in 30% sucrose. The brains were cut serially on a sliding microtome at a thickness of 80 microns. Every 10th section was stained with cresyl-violet and adjacent sections were stained with hematoxylin and eosin (H&E). Near-adjacent sections were stained immunohistochemically for glial fibrillary and acidic protein (GFAP). An immunoperoxidase method was used with a polyclonal rabbit antibody (Dako, Carpinteria, CA) to GFAP at a dilution of 1:2000. Immunoreactivity was visualized using affinity purified goat anti-rabbit secondary antibody, rabbit peroxidase-antiperoxidase, and diaminobenzedine as chromogen. The histological slides were imaged with a Nikon microscope (Nikon Instruments Inc., Melville, NY, USA) and a ProgRes C14 Plus digital camera (Jenoptik, Rochester, NY).
Progres c14 plus digital camera
Manufactured by Jenoptik
Sourced in United States, Germany
The ProgRes C14 Plus is a digital camera designed for microscopy applications. It features a high-quality CMOS sensor with 14 megapixel resolution, allowing for the capture of detailed images. The camera is capable of capturing images with a high dynamic range and low noise levels, providing clear and accurate representations of the subject matter.
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Lab products found in correlation
2 protocols using progres c14 plus digital camera
1
Histological Analysis of Mouse Brains
2
Immunolabeled Protocol Imaging Workflow
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Immunolabeled overview preparations as well as histological preparations were viewed, analyzed and documented using either an Axioskop 2 compound light microscope (Zeiss) with a stem-mounted SpotFlex digital camera (Diagnostic Instruments) or an Axio Imager.A1 compound light microscope (Zeiss) with a ProgRes C14 plus digital camera (Jenoptik, Germany). In order to improve the depth of field, selectively focused images were stacked and combined using Helicon focus 4.2.7 software (Helicon Soft Ltd.) in some instances. Confocal imaging was performed using either a LSM 710 (Zeiss) or a CLSM 2 (Leica) confocal microscope, and 3D images were created using ImageJ (NIH).
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