The human monocyte cell line THP-1 and CRC cell lines (SW480, HCT116, LoVo, and RKO) were obtained from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). Lentiviruses carrying full-length CPEB3 or short hairpin RNA (shRNA_CPEB3) sequences targeting against human CPEB3 mRNA and matched negative controls were constructed by the Shanghai Institute of Biochemistry and Cell Biology. SW480, HCT116, LoVo and RKO cells were transfected with the indicated lentivirus overnight, then 2 μg/mL puromycin was added after 72 h of transfection to obtain stably transfected CRC cells. For macrophage generation, THP-1 cells were treated with 100 ng/mL phorbol- 12-myristate-13-acetate (PAM) (Beyotime, Shanghai, China) for 12 h to differentiate into adhered macrophages. To obtain TAM supernatants, CRC cells were seeded in 0.4-μm pore inserts, then transferred to a 6-well plate seeded with THP-1 macrophages in advance and co-cultured for another 24 h. For co-culture experiments, stably transfected CRC cells were co-cultured with THP-1 macrophages for another 24 h.
Thp 1
Manufactured by Beyotime
Sourced in China
The THP-1 is a cell line derived from an acute monocytic leukemia patient. It is a widely used model for studying monocyte and macrophage biology.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using thp 1
1
Generating Tumor-Associated Macrophages from THP-1 Cells
2
Modulating CRC Progression via CPEB3
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The human monocyte cell line THP-1 and CRC cell lines (SW480, HCT116, LoVo, and RKO) were obtained from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). Lentiviruses carrying fulllength CPEB3 or short hairpin RNA (shRNA_CPEB3) sequences targeting against human CPEB3 mRNA and matched negative controls were constructed by the Shanghai Institute of Biochemistry and Cell Biology. SW480, HCT116, LoVo and RKO cells were transfected with the indicated lentivirus overnight, then 2 μg/mL puromycin was added after 72 h of transfection to obtain stably transfected CRC cells. For macrophage generation, THP-1 cells were treated with 100 ng/mL phorbol-12-myristate-13-acetate (PAM) (Beyotime, Shanghai, China) for 12 h to differentiate into adhered macrophages. To obtain TAM supernatants, CRC cells were seeded in 0.4-μm pore inserts, then transferred to a 6-well plate seeded with THP-1 macrophages in advance and co-cultured for another 24 h. For co-culture experiments, stably transfected CRC cells were co-cultured with THP-1 macrophages for another 24 h.
3
Modulating CRC Progression via CPEB3
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human monocyte cell line THP-1 and CRC cell lines (SW480, HCT116, LoVo, and RKO) were obtained from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). Lentiviruses carrying fulllength CPEB3 or short hairpin RNA (shRNA_CPEB3) sequences targeting against human CPEB3 mRNA and matched negative controls were constructed by the Shanghai Institute of Biochemistry and Cell Biology. SW480, HCT116, LoVo and RKO cells were transfected with the indicated lentivirus overnight, then 2 μg/mL puromycin was added after 72 h of transfection to obtain stably transfected CRC cells. For macrophage generation, THP-1 cells were treated with 100 ng/mL phorbol-12-myristate-13-acetate (PAM) (Beyotime, Shanghai, China) for 12 h to differentiate into adhered macrophages. To obtain TAM supernatants, CRC cells were seeded in 0.4-μm pore inserts, then transferred to a 6-well plate seeded with THP-1 macrophages in advance and co-cultured for another 24 h. For co-culture experiments, stably transfected CRC cells were co-cultured with THP-1 macrophages for another 24 h.
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