Cyan analyzer
The CyAn analyzer is a flow cytometry instrument designed for high-performance cell analysis. It is capable of detecting and measuring multiple parameters of individual cells or particles in a sample. The CyAn analyzer uses light scattering and fluorescence techniques to provide quantitative data on size, granularity, and the presence of specific cellular markers.
Lab products found in correlation
6 protocols using cyan analyzer
Intracellular Protein Staining in ESCs
SSEA-1 Expression Analysis by Flow Cytometry
Axl Expression Analysis in Lung Cancer
nonsmall cell lung cancer cell line NCI-H249, obtained from American
Type Culture Collection (Manassas, VA), were grown in RPMI-1640 supplemented
with 10% fetal bovine serum. For FACS analysis, adherently grown A549
cells were detached by applying nonenzymatical citric saline buffer.21 (link) NCI-H249 cells were grown in suspension. To
minimize nonspecific uptake, the procedures were performed on ice.
Aliquots of cells were blocked with 10% normal goat serum, incubated
with anti-Axl primary antibody h173 (kindly provided by Vasgene Therapeutics
Inc., Los Angeles, CA) produced using composite human antibody technology20 (link) and goat antihuman Alexa Fluor 488 (Invitrogen,
Paisley, Scotland) in sequence. Subsequently, cells were measured
by flow cytometry (CyAn analyzer, Beckman Coulter). Cells without
the incubation of primary antibody were used as negative controls.
Efficient DNA Repair Evaluation via Transfection
Cell Cycle and Death Analysis of Colon Cancer Cells Treated with Robenidine
Secondary to spectral overlap of RB with propidium iodide, DAPI was used to stain DNA and label dead cells. For cell cycle analysis, harvested cells were fixed with 80% ethanol on ice for 30 min and then incubated with 1 ml of DNA staining solution (1 μg/ml DAPI, 0.1% NP-40 in PBS) at room temperature for 30 min. DNA content was determined with FACS on a CyAn analyzer (Beckman Coulter, Brea, CA, USA) and analyzed with the FlowJo software (Ashland, OR, USA).
Cell death was determined with an Annexin V-Biotin Apoptosis Detection Kit. Briefly, cells were first stained with biotin-conjugated Annexin V in 1x binding buffer at room temperature for 15 min and then incubated with streptavidin-APC for 30 min. After being washed with PBS, cells were resuspended in PBS containing DAPI (0.2 μg/ml) for 5 min followed by FACS analysis. Cell death profile was analyzed with the FlowJo software. Necrotic cells were defined as DAPI+ and DAPI+/Annexin V+, and apoptotic cells were defined as Annexin V+ only.46 (link)
FACS Analysis of Adherent and Suspension Cells
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